胡椒园间作槟榔体系小气候对胡椒产量的影响

为确定胡椒园间作槟榔体系影响胡椒产量的关键小气候因子,在海南胡椒园间作槟榔优势区开展4种间作模式与单作胡椒园小气候因子对产量影响的为期4 a的试验,研究不同间作密度下,对胡椒产量有主要贡献的灌浆期的叶片光合作用参数、每日最高和最低气温、不同深度土层土壤温度和含水量,分析胡椒园间作槟榔体系小气候因子对胡椒产量的影响。结果表明:合理间作可以提高胡椒产量,其中间作槟榔株数为765株/hm2的T2处理可以显著提高胡椒产量38.71%,为增产效应最大的处理。分析T2处理增产的原因,发现光强影响的关键光合参数气孔导度对产量有极显著正效应,光合有效辐射、光合速率对产量有显著正效应,T2处理显著提高了这三个光合参数;高温干旱的灌浆期胡椒园日最高温和升温幅度与产量负相关,T2处理显著降低了这两个参数,使胡椒产量增加;表层土壤温度与产量呈负相关关系,T2处理显著降低了表层土壤温度,对胡椒根系有保护作用。综合分析,发现光合有效辐射、日最高温、升温幅度和表层土壤温度是影响胡椒产量的关键小气候因子,所以,这几种小气候因子是用来确定间作模式是否合理的关键参数。     

信阳绿茶主食面包的研制

将信阳毛尖绿茶研磨成粉添加到主食面包中制备信阳绿茶主食面包,研究不同绿茶粉添加量对主食面包比容、感官品质、水分含量及酸度的影响。结果表明,绿茶粉最佳添加量为面包粉质量的3%,此时得到的绿茶主食面包比容为5.75 m L/g,感官品质评分98分,水分含量32.7%,茶多酚含量0.386 g/100 g,酸度2.98°T;绿茶面包表皮完整且丰满,无焦斑,色泽均匀,稍有茶叶的黄绿色,组织紧密有弹性、耐咀嚼,有茶叶的清香,无茶苦味,且具有较好的营养保健功能,市场前景广阔。     

黑蒜非发酵法制备工艺与抗氧化活性研究

为了提高黑蒜的生产效率、降低黑蒜的生产成本,将蒸制工艺与烘制工艺相结合,设计并优化了一种非发酵黑蒜制备工艺。研究结果表明,非发酵黑蒜的最佳制备工艺参数为:蒸制温度127℃、蒸制时间70 min、烘制温度95℃、烘制时间6.5 h,在此工艺条件下制得的黑蒜总酚质量比为11.15 mg/g,含水率为27.41%,还原糖和总酸质量比分别为7.87 g/(100 g)和36.09 g/kg。在0.06~0.18 mg/m L质量浓度范围内,黑蒜的还原能力是维生素C的1.4倍以上,说明黑蒜具有很强的抗氧化活性。与发酵工艺相比,总酚等活性成分含量相近,但极显著地缩短了黑蒜的制备时间、提高了生产效率、降低能耗,为功能性蒜制品的开发利用提供了技术基础。     

浙江乌牛早春茶始摘期及霜冻风险区划

为了解浙江省乌牛早春茶初始采摘期的时空分布特征和减少遭受春季霜冻的风险,应用1971—2014年浙江省71个常规站日资料和2005—2014年共2094个气象自动区域站小时观测资料,以乌牛早春茶发育热量为始摘期区划指标和乌牛早春茶各级冻害总灾损率为霜冻害区划指标,应用GIS技术,采用距离权重反比法(IDW,Inverse Distance Weighted),得到浙江省乌牛早春茶始摘期区划图和乌牛早春季霜冻风险区划分布图。结果表明:浙江全省乌牛早春茶始摘期在2月上旬—3月上旬,从南到北时间递增,温州沿海地区最早,29°N以南为2月中旬,30°N以北地区最晚。浙江沿海、盆地、低丘陵地区适宜种植乌牛早茶,海拔高的地区遭受春季霜冻风险大,不适宜种植乌牛早茶,区划结果将为茶叶主管部门和农技推广部门指导乌牛早种植的布局调整提供重要参考价值和科学依据。     

茉莉花香气成分及其加工应用研究进展

笔者阐述了茉莉花香气成分的释放机理,分析了影响茉莉花香气成分的因素,指出茉莉花香气成分的释放主要是酶促反应的进行,是由花香前体物质在温度、水分、氧气等作用下经生物酶水解释放出来,认为品种、花的开放程度、产地、环境因子、提取方法都对茉莉花香气成分的组成和含量具有不同程度的影响,其中以花的开放程度、环境因子和提取方法最为明显。文章还对茉莉花在食品、茶叶、香烟及其他方面的应用做了概述,最后对茉莉花的理论研究以及开发新产品方面做了展望。     

Multilocus sequence typing analysis of Italian Xanthomonas campestris pv. campestris strains suggests the evolution of local endemic populations of the pathogen and does not correlate with race distribution

Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot in Brassicaceae. It is widespread in Italy and severe outbreaks occur under conditions that favour disease development. In this study a multilocus sequence typing approach (MLST) based on the partial sequence of seven loci was applied to a selection of strains representative of the main areas of cultivation and hosts. The aim was to investigate whether the long tradition of brassica crops in Italy has influenced the evolution of different Xcc populations. All loci were polymorphic; 14 allelic profiles were identified of which 13 were unique to Italian strains. Based on the seven loci, the most common genotype within the Italian Xcc strains (AP1) was also the most representative genotype found in worldwide Xcc strains. This genotype was included in a new clonal complex in addition to three other clonal complexes already identified in Xcc populations. The phylogenetic reconstruction using a concatenated dataset of four conserved protein-coding genes, dnaK, fuyA, gyrB and rpoD, showed that the Italian strains belonged to two genetic groups. Physiological races were also investigated for the first time in Italy. The race structure of Xcc was determined by inoculating eight differential Brassica lines belonging to five species and showed that, in Italy, race 4 is the most widespread, followed by races 1 and 6. No correlation was found between allelic profiles, host of isolation, geographical origin and races, although a prevalent race was identified within the same clonal complex.     

中国茶树炭疽菌属病害研究进展及展望

茶树是一种重要的经济作物,广泛种植于热带和亚热带地区。随着茶产品经济价值的提高,茶叶生产安全备受关注。炭疽菌是引起茶树病害的病原菌之一,严重影响茶叶产量和茶产品品质,威胁我国茶产业的良性发展。利用抗病品种是防治茶树病害最为经济、有效的措施。目前,茶树炭疽菌种名使用混乱,茶树与炭疽菌的互作研究也较为薄弱,而简单依靠药剂防治病害也对茶叶质量安全造成一定的影响。本文梳理并明确了危害茶树的炭疽菌种类;总结了现有的茶树和炭疽菌互作的研究成果,提出识别免疫(effector triggered immunity,ETI)模式是茶树防御炭疽菌的主要机制;对茶树病理学存在的问题进行了归纳并提出解决办法,并展望了深入开展茶树抗病机理研究的思路,以期为制定茶园绿色防控技术及选育茶树抗病良种提供科学参考。     

Evaluating diverse systems of tobacco genetic resistance to Phytophthora nicotianae in Yunnan,China

Black shank, caused by the soilborne pathogen Phytophthora nicotianae, is one of the most devastating diseases affecting tobacco production in China. The most effective strategy for reducing economic loss from this pathogen is development and use of resistant tobacco varieties. Multiple sources and systems of resistance have been developed in the Western Hemisphere; however, populations of P. nicotianae are variable around the world, including the predominance of different races. Different P. nicotianae isolates may react differently on tobacco plants with different systems of resistance, a possibility that could complicate the breeding of cultivars with resistance that is effective in different tobacco production regions worldwide. The objective of this research was to evaluate an array of tobacco germplasm possessing different systems of genetic resistance to black shank disease in tobacco-growing regions of Yunnan, China. Resistance types included simply inherited resistance mechanisms introgressed from wild Nicotiana relatives and polygenic partial resistance systems of N. tabacum origin. The loci of Wz exhibited high level resistance to black shank in the five diverse disease environments in Yunnan, China. K326 Php/−Wz/− genotype and Beinhart 1000 exhibited the greatest levels of resistance in both 2015 and 2016. Field observed results for 13 tobacco genotypes were highly correlated with those tested in growth chamber evaluation. These findings suggest that both Wz− and Beinhart 1000-mediated resistance have important commercial value in flue-cured tobacco breeding programmes in China. Cultivars developed for black shank resistance in China may also have utility in other tobacco-growing areas.     

基于Lucid多途径检索的油茶害虫诊断APP开发与实现

油茶是亚热带地区重要的木本油料作物,近年来随着种植面积的不断扩大和集约化经营,病虫害问题日益严重,对油茶的产量和品质造成不同程度的影响。本研究通过野外调查、文献查阅、种类鉴定等工作,整理了21种油茶主要害虫的形态特征、生物学习性、为害特点、防治措施等信息,并拍摄相应害虫及为害状的高清照片1 600张。采用Lucid智能诊断系统,构建Fact Sheet Fusion基础信息数据库,并针对多途径检索方式提取害虫"为害方式"、"为害部位"、"为害高峰期"、"形态特征"等4个1级特征组,以及10个2级特征和18个3级特征,共组成102个特征状态,构建了油茶害虫的快速诊断系统。在此基础上,转换得到基于Android系统的手机应用APP,为林农和森防一线工作者对油茶害虫快速识别和防治提供便捷服务,从而促进油茶产业持续健康发展。