羊生长激素的提取及生物化学和生物活性的测定

600 g 冰冻羊垂体经过酸性溶液提取、硫酸铵的分级分离和 DEAE-纤维素柱层析得纯品(1),其得率为0.08g/kg,最后将部分纯品(1)过 Sephadex G-100分子筛,得到高纯的羊生长激素[纯品(2)]。提取的羊生长激素具有相当高的理化均一性,纯品(2):聚丙烯酰胺凝胶电泳呈现一条主带和二条酸性小带;聚丙烯酰胺等电聚焦电泳呈现二条带,其等电点分别为 pH7.35和 pH7.40,SDS—电泳呈现二条很难分开的带,其平均分子量为20 100道尔顿;用DNS-CI 法测得的 N-末端氨基酸是2个,丙氨酸和苯氨酸。纯品(1)理化均一性稍差:SDS—电泳较纯品(2)多出两条分子量低的带;等电聚焦电泳多出六条酸性小带。除此之外,其他理化性质同纯品(2)。用大白鼠胫骨实验测定生物活性,测得纯品(1)可使垂体大白鼠胫骨软骨盘的宽度相对增加79%。未对纯品(2)进行生物活性测定。用羊催乳素放射免疫药盒测定,纯品(1)和纯品(2)与羊催乳素均无交叉反应。     

An Epidemiologic and Economic Study of Respiratory Diseases in Two Conventional Danish Swine Herds. I: Prevalence of Respiratory Lesions at Slaughter and Their Effects on Growth

A total of 578 slaughter pigs from 2 Danish conventional farrow-to-finish operations (Herds A and B) were followed from an age of 14 days to slaughter. Pigs were weighed at 3 weeks intervals and at slaughter and an extended post mortem examination of the plucks was done. Comparison of growth rates in pigs with and without specific types of lesions by the t-test and those with multiple lesions with regression models demonstrated that Mycoplasma-like pneumonia, complicated pneumonia, anterio-ventral pleuritis, fissures and atrophic rhinitis significantly reduced mean daily gain and increased the time required to reach slaughter weight. The total impact of the lesions in Herd A was an estimated reduction in mean daily gain of 27 grams and a 2 day increase in the interval from 14 days of age until slaughter (MDG14). Decreases in MDG14 in Herd B were more substantial, 98 grams and 16.7 days. Reductions in mean daily gains during the interval from the fourth weighing until slaughter were 31 grams in Herd A and 137 grams in Herd B. Chronic dorso-caudal and parietal pleuritis, without other lesions present, had no significant adverse effects on growth rates in either herd. Interactions between lesions did not significantly alter the estimates. The R² values obtained for the regression models showed that the presence, absence or extent of lesions at slaughter explained only 13-27% of the variations in growth rates in the 2 herds.     

苜蓿施用污泥效果的研究Ⅰ对苜蓿生长及元素含量的影响

将兰州市七里河污水处理厂生活污泥施用于人工种植的新疆大叶苜蓿草地，探讨了不同施用量对苜蓿生长及各元素含量的影响。研究结果表明，施用1.0-2.0kg/m^2污泥，苜蓿地上生物量显著增加，第一年增产23％－31％，第二年增产15％－31％。施用污泥苜蓿地上和地下部组织中N，P，Ca,Mg和Mn的含量总体呈增加趋势，Fe含量显著增加，而K和重金属元素Ni、Pb、As和Hg的含量与对照无显著差异，Zn和Cu的积累增多，但地上部组织中重金属元素均未超过植物中的平均含量，施用污泥未对新疆大叶苜蓿造成重金属污染。     

马尾松不同苗木类型与整地方式造林早期效果的研究

通过对不同苗木类型和整地方式的马尾松幼林的造林成活率、生长状况、生物量和根系特征进行测定，结果表明：容器苗可明显提高造林成活率、当年抽梢高和生物量；整地方式对造林当年的马尾松幼林有一定影响，而在第３年则对幼林树高和抽梢高起着明显促进作用。     

阔叶次生林抚育改造初探

安远县国营林场根据生态学原理,对阔叶次生林进行改造,把天然更新和人工改造更新有机的结合起来。通过改造可很快的形成多树种的混交林,林木材积生长提高115％～219％,土壤物理性比杉木纯林好,土壤贮水量比杉木纯林提高57％～92％,有良好的生态经济效益。实践证明,次生林改造是次生林经营的重要措施,是加速培育森林资源的好途径。     

Effect of sodium nitrite and sodium chloride on growth of lactic acid bacteria.

The effect of NaNO2 and NaCl on the growth of 24 lactic acid bacteria strains isolated from vacuum-packed cooked ring sausages were examined by analyzing different growth parameters with Bioscreen. NaNO2 had a very limited effect on the growth of lactic acid bacteria at 50 and 100 mg/l but at 400 mg/l a more pronounced inhibitory effect was found. Bacterial growth was enhanced by 1-2% (w/v) of added NaCl, while NaCl concentrations above 3% (w/v) had a clear inhibitory effect. Leuconostoc isolates seemed to be more sensitive to sodium nitrite and sodium chloride than homofermentative lactobacilli strains. Among homofermentative lactobacilli, the strains resembling Lactobacillus curvatus were more sensitive to NaCl than those resembling Lactobacillus sake.     

Physiological Analysis of Yield Variation in Mungbean (Vigna radiata [Roxb.] Wilczek)

Two mungbean varieties, Guj-1 and PIMS-1 differing in their productivity potential, were examined to investigate their photosynthetic system at various stages of growth in relation to yield with two different dates of sowing. Vertical leaves were found to be beneficial. On the other hand, excessive leaf area during the later stages of growth may be detrimental to yield. Lower LAD at seedling stage and overall high NAR values may be reflected in higher grain yield. NAR declined as season progressed and this, in turn, may be related with increase in LAI. The grain yield was considerably more at the first date of sowing as compared to second date of sowing, the effect was being more pronounced in var. PIMS-1 . Various environmental factors recorded at two different dates of sowing and at various stages of growth indicated that these factors may have influenced the yield potential at two different dates of sowing.     

内蒙古大兴安岭林区野生动物资源开发对策

内蒙古大兴安岭林区野生动物资源十分丰富,在积极保护的基础上,合理开发利用野生动物资源对林区培育新的经济增长点、提供就业机会具有重要意义.本文主要从药用野生动物、毛皮动物、食用动物及野生动物生态旅游产品等几个方面的开发对策进行了探讨.     

灌水量对不同类型晚稻生育及产量影响的初步研究

采取盆栽遮雨人工定量灌水的方法,探讨了常规晚稻和杂交晚稻前后两大生育时期不同灌水量对生育和产量的影响.结果表明:两类型晚稻品种前后两个生育时期节水灌溉都能达到高产之目的,但不同类型和不同生育时期的最佳灌水量和节水效果不同.     

乌鳢生长发育的生态因子及其流行病的防治

对非生物生态因子———水、光照、温度、溶氧、降水等和生物生态因子———食物、流行病和群体密度等对乌鳢生长发育的影响及流行病的防治进行了综述。