用转轮内三元流动计算对泵叶轮进行改型设计

在有厚翼栅奇点解法的基础上，利用一种考虑叶片厚度影响的叶片式水力机械转轮内准三元流动新的分析方法、准确计算了泵叶轮叶片表面上的速度与压力变化分布规律，获得了许多水力性能评估参数；通过评价这些参数，对叶片型线进行了修改，获得了比较满意的结果。为叶轮的改型设计提供了一条较有价值的新途径。     

高速柴油机曲轴扭/弯/纵三维振动的试验研究

利用开发的轴系扭 /弯 /纵三维振动测试装置 ,对典型的直列 6缸车用柴油机进行了轴系扭 /弯 /纵三维振动的测试研究。通过对轴系振动的测试结果进行幅频特性分析以及相干函数分析 ,从试验研究的角度分析了轴系扭 /弯 /纵三维振动的相互影响的特点。同时 ,研究了轴系的三维振动与机体裙部振动的关系。     

齿轮三维模型的参数化设计技术

齿轮是汽车、拖拉机中常用的传动零件,在三维设计中,需要对渐开线方程进行转换,由转换后的方程生成齿形,其整个设计过程非常繁琐。本文详述了方程的转换过程及齿轮的参数化设计方法,可以通过输入不同的齿轮参数,即可获得相应的齿轮,从而大大节约设计时间。该方法还可应用于所有系列化的通用件和标准件三维设计中。     

云龙河拱坝施工过程的三维有限元

采用三维弹塑性非线性有限元法，对云龙河拱坝施工过程的实际浇筑、封拱和水库蓄水过程的坝体应力及变形进行了仿真分析，同时分析了施工过程的坝肩层间错层带的变形。计算结果表明施工过程的坝体变形较小，坝体有限元等效应力满足规范要求，坝肩层间错层带错动变形很小，说明大坝结构设计合理，坝肩无错动危险。计算成果为工程项目的实施提供了技术依据，同时对类似工程分析具有借鉴作用。     

惯性沉降分离室内三维定常气流流动数值计算

为了解气流在4ZTL-1800型割前摘脱稻（麦）联合收获机惯性沉降分离室内的运动规律，从流体力学和湍流理论出发，建立了气体运动微分方程式及k-ε湍流模型，并利用Fluent软件对惯性沉降分离室内三维定常气流进行了数值计算，得到了气体在惯性沉降分离室内的流动特征。根据其特征对分离室结构进行了改进设计，数值计算结果表明改进后惯性沉降分离室内的气流流场得到了改善。     

Mechanism of Fuzilizhong decoction alleviating inflammatory damage of rats with non-alcoholic fatty liver disease

AIM To observe the effect of Fuzilizhong decoction on the inflammatory damage of non-alcoholic fatty liver disease （NAFLD） rats and to explore its mechanism. METHODS SPF male SD rats were randomly divided into 6 groups： control group, model group, high dose （20 mg·kg^-1·d^-1）, middle dose （10 mg·kg^-1·d^-1）, low dose （5 mg·kg^-1·d^-1） Fuzilizhong decoction group and Yishanfu （30 mg·kg^-1·d^-1）group, 8 rats in each group. A NAFLD rat modelwas established by intragastric administration of fat emulsion for 4 weeks. Then the drug was given for 4 weeks in each treatment group. HE staining was performed to observe the histopathological changes of the rat liver.The serum levels of interleukin-2（IL-2）, IL-6 and tumor necrosis factor-α（TNF-α） were measured by ELISA. The expression of toll like receptor 4（TLR4） and NF-κB p65 in liver tissues at mRNA and protein levels was determined by RT-qPCR and Western bolt,respectively. RESULTS Compared with control group, the inflammatory damage of liver tissue was more serious, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression TLR4 and NF-κB p65 in liver tissues were significantly increased in model group（P<0.05）. However, compared with model group, the liver pathological changes in each treatment group were significantly relieved, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression of TLR4 and NF-κB p65 in liver tissues were significantly reduced（P<0.05）.In addition, the changes of TLR4 and p-NF-κB p65 protein levels in liver tissue were consistent with the changes of TLR4 and NF-κB p65 mRNA. CONCLUSION Fuzilizhong decoction attenuates the inflammatory damages of NAFLD in rats by inhibiting TLR4/NF-κB p65 signaling pathway.     

p300/p53/Smad3 pathway involved in aging-related atrial fibrosis in human atrial fibroblasts

AIM To investigate the role of p300 in aging-related atrial fibrosis in human atrial fibroblasts （HAFs） and its potential mechanism. METHODS HAFs were obtained from human left atrial tissue, and the senescence model was established by cell passage. Senescence-associated β-galactosidase （SA-β-Gal） staining was used to detect the cell senescence, and Western blot was used to determine the protein levels of p300, p53, Smad3 and other senescence and fibrosis associated proteins in HAFs. RESULTS Compared to passage 3 HAFs, the proportion of senescent cells, and the protein levels of p300, p53, p-Smad3 and other senescence and fibrosis associated proteins were increased in HAFs at passage 7 and 11 （P<0.05）. After treated with curcumin （a p300 inhibitor） or transfection with p300 small-hairpin （sh） RNA plasmid, the protein levels of p300, and the senescence and fibrosis associated proteins were decreased in HAFs at passage 7（P<0.05）. Up-regulation of p300 by transfection with p300 over-expression plasmid increased the protein levels of p53, Smad3 and MMP-2 in HAFs at passage 3 （P<0.05）. CONCLUSION p300/p53/Smad3 signaling pathway plays an important role in aging-related atrial fibrosis in HAFs.     

Effects of naringenin on PI3K/AKT signaling pathway and endoplasmic reticulum stress and its related apoptotic pathways in rats with myocardial ischemia/reperfusion injury

AIM To observe the effect of naringenin on cardiac injury in ischemia/reperfusion （I/R） rats, and to explore whether the role of naringenin is involved in PI3K/AKT signaling pathway and endoplasmic reticulum stress and its related apoptotic pathways. METHODS SD rats （n=48） were randomly divided into sham operation （sham） group, model （I/R） group, naringenin treatment （NAR） group and naringenin+LY294002 （NL） group. Myocardial I/R injury model was prepared by ligation of left anterior descending coronary artery of rats for 30 min followed by reperfusion for 120 min. After reperfusion, the serum levels of cardiac troponin I （cTnI） was measured by ELISA. HE staining, TTC staining and TUNEL staining were used to detect the myocardial histopathological changes, myocardial infarction area and myocardial cell apoptotic rate. The mRNA levels of endoplasmic reticulum stress-related indicators glucose-regulated protein 78 （GRP78）, C/EBP homologous protein （CHOP） and caspase-12 were detected by RT-qPCR. The protein levels of cleaved caspase-3, GRP78, CHOP, caspase-12, p-PI3K and p-AKT were determined by Western blot. RESULTS Compared with I/R group, the serum content of cTnI, myocardial pathological damage, myocardial infarction area and myocardial cell apoptotic rate were significantly reduced （P<0.05）, the protein levels of cleaved caspase-3, GRP78, CHOP and caspase-12 were decreased （P<0.05）, and the protein levels of p-PI3K and p-AKT were increased in NAR group （P<0.05）. LY294002 attenuated the protective effect of naringenin to some extent. CONCLUSION Naringenin reduces myocardial I/R injury in rats possibly by activating PI3K/AKT signaling pathway and subsequently regulating endoplasmic reticulum stress and its related apoptotic pathways.     

3DMAX应用下的智能插秧机外观优化

为进一步提高智能插秧机的结构布局协调性与感染力水平,利用3DMAX应用平台进行插秧机外观优化研究。考虑土壤湿润程度对插秧刀具的影响等因素,结合插秧机分插机构传动部件与插植臂等执行部件的相互关系与约束条件,以偏心齿轮啮合传动特性,建立用于智能插秧机外观优化的数学模型,得到关键部件与机构的三维物理模型与无缝装配模型。从结构细化设计与外观造型设计两大角度分别对插秧机体、驾驶座、车轮、插秧装置等曲线参数优化,插秧作业场景与插秧特征提取及组件元素3D建模等操作进行3DMAX场景下智能插秧机的外观呈现与界面设计布局,实现了智能插秧机的3D可视化管理与动画效果展示,且动作一致性较好,设计合理可行。农机三维建模技术对准确实现结构优化有很好的预知功能,对大型农机设备结构设计搭建人机交互虚拟平台有一定的参考价值。