Geographical variation of nuclear genome RFLPs and genetic differentiation in foxtail millet, Setaria italica (L.) P. Beauv.

Sixteen RFLP loci in 62 landraces were assayed to study geneticdifferentiation in foxtail millet, Setariaitalica (L.) P. Beauv. Among 52 bands, 47 werepolymorphic among foxtail millet landraces. A dendrogram constructedbased on RFLPs was divided into five major clusters (clusterI–V). Clusters I and II contained strains mainly from EastAsia. Cluster III consisted of strains from subtropical and tropicalregions in Asia such as Nansei Islands of Japan, Taiwan and thePhilippines and India and cluster IV consisted of some strains fromEast Asia, a strain from Nepal and a strain from Myanmar. Cluster Vcontained strains from central and western regions of Eurasia such asAfghanstan, Central Asia and Europe. Chinese landraces wereclassified into four clusters. These results indicate that foxtailmillet landraces have differentiated genetically between differentregions and that Chinese landraces were highly variable.     

Genetic variability in foxtail millet, Setaria italica (L.) P. Beauv.: Identification and classification of lines with RAPD markers

The DNA of 37 lines of Setaria italica, representative of Eurasian diversity, were used for the detection of polymorphism using the random amplified polymorphic DNA (RAPD) method. Four 10-mer primers, differing from each other by one or two G-C inversions, were used. Each one gave specific RAPD products. Twenty-five bands were polymorphic and allowed the identification of 33 different genotypes. A factorial analysis of correspondence was performed on the presenc-absence data, through which three genetic groups could be identified. These genetic groups were closely related to the geographical origin of the different lines: one central European and two Asiatic groups (the first Asiatic lines originating in latitudes below 35°N and the second comprising the Asiatic lines originating in latitudes above 35° N). The lines originating from western Europe were very variable and were dispersed in the three genetic groups described previously.     

Subconjunctival nodule due to Setaria equina erratic migration in a horse: First case report

An 18‐month‐old Arabian‐English filly resident in southwest France was referred for evaluation of a conjunctival mass in the right eye (OD). A pink, solid, and mobile nodular formation, measuring approximately 1.2 × 0.8 cm was found under the superior nasal bulbar conjunctiva during an ophthalmic examination that was otherwise normal. The mass was surgically removed using a standing procedure. Cytological examination of fine‐needle aspirates from the mass revealed a mixed eosinophilic‐lymphocytic inflammation. Histological examination confirmed the dense and diffuse eosinophilic‐lymphocytic infiltrate of the mass, and it revealed several cross sections of a parasitic nematode. The morphometric diagnosis identified an immature form of a filarial worm, and molecular analysis of the mitochondrial cytochrome c oxydase subunit 1 (cox1) and 12S rRNA gene sequences led to further identification of the specimen as Setaria equina. Microfilaremia was not observed on fresh blood smears. There have been no signs of local recurrence after 18 months, nor any evidence of intraocular involvement. To the authors’ knowledge, this is the first documented case of subconjunctival setariasis due to S equina in a horse.     

平流层辐射SP_3谷子雄性细胞发育的研究

对平流层辐射处理ＳＰ３谷子和对照ＣＫ３谷子雄性细胞发育的研究表明,雄性细胞正常发育过程从孢原细胞开始,经初生造孢细胞、次生造孢细胞、小孢子母细胞、二分体、四分体和单核小孢子中央期、单核小孢子液泡期（单核靠边期）、单核小孢子成熟期直到二细胞花粉、三细胞成熟花粉结束。其中四分体形成属连续型。雄性细胞异常发育有几种情况：小孢子母细胞强烈液泡化,细胞质收缩解体,不能进入减数分裂;小孢子母细胞液泡化,呈能进     

凯氏丝状线虫(Setaria Kabargi Kadenazzi)在我国的首次发现

本文首次报道寄生于我国野生动物林麝(Moschus berezovskii)腹腔中的凯丝状线虫(Setaria kabargj).     

Broadening weed control with imazamethabenz combinations

Broad-spectrum weed control by use of imazamethabenz (AC 222 293) in herbicide combinations was investigated in the greenhouse. Imazamethabenz at rates ranging from 0·1 to 0·4 kg a.i./ha effectively controlled wild oats (Avena fatua L.). Additional control of redroot pigweed (Amaranthus retroflexus L.) was achieved when imazamethabenz, plus octoxynol surfactant, was applied in combination with MCPA (amine or ester) or commercial MCPA mixtures (bromoxynil/MCPA and cyanazine/MCPA). Only inclusion of propanil/MCPA in the combination reduced wild-oat control by imazamethabenz at the lower rate (0·1 kg/ha). Control of green foxtail (Setaria viridis (L.) Beauv.) was also obtained by use of fenoxaprop-ethyl in combination with imazamethabenz (plus octoxynol). Three-way mixtures of imazamethabenz (or plus octoxynol), MCPA alone or mixture, and fenoxaprop-ethyl may provide practical control of grass and broad-leaved weeds in the field.     

狗尾巴草多酚提取及抗氧化性研究

【目的】研究狗尾巴草中多酚的最佳提取条件及其抗氧化性。【方法】采用乙醇浸提法,探讨了浓度、温度、时间和料液比等提取条件对提取多酚的影响,并通过正交实验优化条件。【结果】结果表明,在最佳条件乙醇浓度为60%,提取时间为2.0h,料液比为1∶20,温度为70℃时,提取的多酚含量为2.806mg/g,实验结果还表明,从狗尾巴草中提取的多酚清除羟基自由基的作用较强。【结论】此工艺提取效果较好,多酚含量较高。     

高湿对农田狗尾草种子发芽特性及其幼苗生长的影响

以狗尾草种子为材料,研究高温(45℃)、相对湿度(50％、60％、70％、80％)处理对种子发芽及幼苗胚芽鞘生长的影响,为揭示狗尾草成功占据休耕地提供依据.研究表明:在50％、60％和70％湿度条件下,老化lld的种子萌发率最高,说明增加湿度可有效解除狗尾草种子休眠.在4个湿度下老化lld的狗尾草种子萌发率变异系数降至最低,死亡率最高,休眠率则呈现由低变高的趋势,表明种子出苗整齐程度和诱导进入次生休眠受高湿条件的影响.老化湿度对胚芽鞘生长影响不明显.     

Assessment of Genetic Relationship of Foxtail Millet with Its Wild Ancestor and Close Relatives by ISSR Markers

Inter simple sequence repeat (ISSR) analysis was applied to samples of foxtail millet and its wild ancestor and other close relatives of the genus Setaria in order to detect domestication-related geogr...