犊牛睾丸组织培养及PGP 9.5基因的检测

收集新生牛睾丸,进行组织学观察与组织的体外培养,在10%血清培养液中培养1周和2周后进行组织学观察与RT-PCR分析。结果表明:新生牛睾丸组织经体外培养1周时,管腔直径增大,精原干细胞数明显增多;体外培养2周时,管腔直径继续扩增,精原干细胞数量减少;在新鲜睾丸组织、培养1周和2周的睾丸组织中PGP 9.5持续表达。     

延边黄牛公牛与阉牛产肉性能的比较

为研究延边黄牛公牛和阉牛产肉性能,选择品种相同、年龄体重相近、生长发育正常的延边黄牛肉牛16头,随机分成2组,每组8头,其中试验组为阉牛,对照组为公牛,育肥373d．结果表明：21月龄时,公牛组和阉牛组平均体重分别为468．63和448．88k,差异显著（P〈0．01）;公牛组和阉牛组的末重分别为542．38和488．63kg,差异极显著（P〈0．01）;公牛组和阉牛组平均日增重分别为0．71和0．57kg,公牛组的平均日增重比阉牛组提高25．4％,差异极显著（P〈0．01）．阉牛组牛肉的外观肉色呈亮红色,属较理想肉色,公牛组牛肉颜色红色稍暗,差异显著（P〈O．05）;阉牛组牛肉脂肪颜色为白色,公牛组白色稍黄,差异不显著（P〉0．05）;大理石状花纹阉牛组优于公牛组．肌内脂肪含量阉牛组比公牛组高23．04％,差异显著（P〈0．05）,粗蛋白含量公牛组比阉牛组高4．41％,差异显著（P〈0．05）,粗灰分含量则差异不显著．     

Evaluation of lipid sources in diets fed to bull trout,Salvelinus confluentus

To aid in development of nutritionally complete diets, a 12‐week experiment was conducted to identify appropriate sources of dietary lipid for bull trout. The basal diet was top‐coated with marine fish oil (MFO) (pollock liver oil), canola oil (CO), linseed oil (LO) or a mixture of canola and linseed oils (CLO) to produce four treatments. Each diet was fed to triplicate groups of fish initially averaging 1.6 g per fish. Weight gain, feed efficiency, survival and carcass proximate composition were not significantly different among fish fed the dietary treatments. However, whole‐body fatty acid percentages varied significantly among fish fed the four diets. Whole bodies of fish fed diets with vegetable oil (VO) contained significantly higher 18:2n‐6, 18:3n‐3 and total n‐6 polyunsaturated fatty acid percentages and significantly lower 20:5n‐3, 22:6n‐3 and total saturated fatty acid percentages compared with fish fed the MFO diet. Whole‐body fatty acid percentages also varied among fish fed VO diets. Despite similar 18:2n‐6 and 20:4n‐6 percentages in the VO diets, fish fed diet CO contained significantly lower 18:2n‐6 proportions and significantly higher 20:4n‐6 proportions compared with fish fed other VO diets. Results of this study suggest dietary fish oil is not required for short‐term rearing of bull trout.     

黄河三角洲稻田养殖牛蛙试验

在３亩稻田中进行了牛蛙、水稻生态养殖、不投饵、不施农药、经９２天养成，平均亩产商品牛蛙１０１．８ｋｇ，亩产水稻４８９．８ｋｇ，每亩盈利１６２３元。１９９４年９月专家鉴定认为：本研究属国内领先水平。     

牛蛙常见病原菌对青蛙的致病性研究

用克氏耶尔森氏菌、奇异变形菌、不动杆菌和爱德华氏菌等４株牛蛙致病菌，在相同条件下分别感染牛蛙和青蛙，均可发病和死亡，且两种蛙感染同一病原菌后的症状及病变也相同，证实青蛙对牛蛙的常见传染病有易感性，是牛蛙病发生的重要传染来源之一。     

Factors affecting habitat selection by a small spawning charr population, bull trout, Salvelinus confluentus: implications for recovery of an endangered species

Bull trout, Salvelinus confluentus (Suckley), populations are declining in many streams of North America and are listed under the Endangered Species Act in the United States. Many small populations are isolated in fragmented habitats where spawning conditions and success are not well understood. Factors affecting habitats selected for redds by spawning bull trout and redd habitat characteristics within Gold Creek, a headwater stream in the Yakima River within the Columbia River basin, Washington State, USA, were evaluated. Most spawning (>80% of the redds) occurred in upstream habitats after dewatering of downstream channels isolated fish. Habitats were selected or avoided in proportions different to their availability. For example, most bull trout selected pools and glides and avoided riffles despite the latter being more readily available. Although preferences suggest influences of prolonged fish entrapment, site fidelity could be important. A habitat with redds commonly contained abundant cover, gravel substratum and higher stream flows. The major factors influencing habitat selection by spawning fish and their persistence in streams of the Yakima and Columbia River regions include entrapment of fish by dewatering of channels and geographical isolation by dams. The goal of the US Government's recovery plan is ‘to ensure the long‐term persistence of self‐sustaining bull trout populations’. Recovery plans linked to provisions for protecting and conserving bull trout populations and their habitats were recommended. Landscape approaches are needed that provide networks of refuge habitats and greater connectivity between populations. Concurrent recovery efforts are encouraged to focus on protecting small populations and minimizing dangers of hybridization.     

黑大豆营养功能及开发豆皮色素的探讨

探讨黑大豆的营养功能,特别是豆皮的抗氧化功能。分析了"粤引大豆一号"新品种的营养价值,比较黑大豆、黑米及黑玉米中生物色素的抗氧化能力,发现黑大豆明显强于后者;对不同粒型、不同产区黄大豆、黑大豆的总酚、总花色苷含量进行比较,发现黑大豆极显著高于前者。阐述了黑豆皮提取物的抗氧化机制,最终提出了具有抗氧化功能的生物色素在营养研究上的重大意义,并对大豆今后的育种目标、种植基地和季节的选择,以及对采用微化技术加工其相关产品提出了具体的建议。     

雄性水牛瘤胃内睾酮的检测及变化规律的研究

采用高压液相色谱（ＨＰＬＣ）法测定了雄性水牛瘤胃液中的睾酮。结果表明,瘤胃上清液及瘤胃细菌菌体中均有睾酮存在,放射免疫分析表明,瘤胃上清液睾酮样免疫活性物质的平均浓度为６３．９±２６．５ｆｍｏｌ／ｍＬ,且春季含量高于夏季（６６．１±３１．０ｖｓ２３．２±１６．５ｆｍｏｌ／ｍＬ）;瘤胃细菌菌体内睾酮样免疫平等互利为２６３．５±１２９．０ｆｍｏｌ／ｍｇ。春夏两季之间无明显的季节性变化（２９８．２±１５     

麦饭石对利木赞种公牛精液品质影响的研究

在种公牛饲料中添加麦级石饲喂利木赞种公牛，进行对其精液品质影响的研究，结果表明；添加１．５％麦饭石可以代替种公牛饲料中的复合矿物质添加剂部分，而且对精液品质、受胎率无任何影响．说明麦饭石可用于反刍动物的矿物质添加剂．     

丹麦黑白花种公牛与不同品系黑白花母牛杂交组合配套的研究

本研究是“优秀种公牛高频采精及其机理研究”课题的一部分，目的是为深圳光明华侨畜牧场及其它奶牛场的奶牛生产选择最优秀种公牛和奶牛生产确定最佳组合配套。本研究选择了丹麦黑白花３头种公牛与中国黑白花母牛，新西兰黑白花母牛，中新杂黑白花母牛进行系间杂交，观察民收订了３０５头其后代女儿的生长发育和产奶量，乳脂率等主要经济性状资料。经统计分析，进行了杂交组合间和母女比较，并对杂交组合的产奶量和乳脂率进行了显著