间接ELISA检测鸭疫里氏杆菌抗体的研究

以提取的血清１型鸭疫里氏杆菌（Ｒ．ａ）Ｊｂ２株的脂多糖作为包被抗原,成功地建立了一种检测１型Ｒ．ａ抗体的间接ＥＬＩＳＡ方法,特异性试验和重复性试验证明此方法的特异性高、重复性好。并比较了间接ＥＬＩＳＡ方法、间接血凝试验、玻片凝集试验和琼脂扩散试验对抗体检出的敏感性,结果表明间接ＥＬＩＳＡ方法最敏感,人工感染后的鸭第７２小时即可检出血清抗体为阳性（３／４）;其次是间接血凝试验,第９６小时即可检出５０％（２／４）;玻片凝集试验在感染９天后才出现阳性（２／３）;而琼扩试验在感染后１１天仍为阴性反应。应用建立的间接ＥＬＩＳＡ方法,初步调查了南京地区鸭场此病的感染情况,发现所调查的１３日龄以前雏鸭的阳性率为０,而２１～３５日龄鸭的阳性率为８７３％。     

丝石竹优良单株快速繁殖的研究

本文报道丝石竹优良单株离体快速繁殖和规模化育苗技术。取其切花后萌芽苗的嫩茎段为外植体，培养在ＯＭ培养基上。在附加６—ＢＡ０．５ｍｇ／Ｌ的ＯＭ培养基上，腋芽的诱导和生长最好；在附加６—ＢＡ４＋ＮＡＡ０．０１的ＯＭ培养基上，芽的增殖效果最佳，增殖倍数为２４．４６；ＯＭＯ（不含任何植物激素的ＯＭ培养基）最利于壮苗；在ＯＭ培养基中附加ＩＢＡ０．１～０．３，不定根诱导和生长效果最佳，生根率达９８．６％。试管苗移入珍珠岩中生长最好，移植成活率为９２％。     

小鹅瘟病毒荧光RPA恒温快速检测方法的建立与应用

旨在建立一种以重组酶聚合酶扩增技术（RPA）为基础的快速检测方法,用于小鹅瘟病毒的快速检测。小鹅瘟是一种常见的水禽传染病,严重危害我国养鹅业的健康发展。为了快速准确对小鹅瘟进行诊断,减少该病的危害,本研究以小鹅瘟病毒VP3基因保守片段为靶点,利用重组酶聚合酶扩增技术（RPA）建立了一种准确高效的小鹅瘟病毒RPA恒温快速检测方法,并对检测方法的灵敏度、特异性进行评价,并与传统PCR和传统RT-PCR方法进行比较。结果表明,该检测方法具有较高的灵敏度,可检测到10 copies/μL的病毒核酸;具有良好的特异性,只特异性地扩增鹅细小病毒,而与鹅副黏病毒、鹅源鸭瘟病毒、小鹅流行性感冒病毒、鹅副伤寒病毒、大肠杆菌和曲霉菌均未发生交叉反应;同时该方法重复性检测的变异系数低于6%,具有很好的重复性。阳性符合试验表明该检测方法与荧光定量PCR符合率为99%。该方法可以很好地应用于小鹅瘟的大规模临床样本检测,为小鹅瘟病毒的高通量检测和流行病学调查提供技术手段。     

芳樟工厂化育苗技术研究

通过对芳樟工业原料林良种茎尖的离体培养和繁殖 ,探讨芳樟茎尖诱导、分化、不定芽生长分化、生根的适宜的培养基和适宜的培养条件。结果表明 :适宜芳樟茎尖诱导培养基为MS +6 -BAa～ 2amg .L-1+NAAbmg .L-1,诱导率达 70 %以上 ,适宜分化丛生芽的理想培养基为改良MS +6 -BAa′～ 2a′mg .L-1+NAA0～ 2bmg .L-1+GA0～cmg .L-1芽年增殖系数达4 5 12 以上 ,有效芽苗率达 80 %以上 ,适合生根培养基为 1/2改良MS +NAAb′mg .L-1+IBA0～b′mg .L-1或 1/2改良MS +IBAb′mg .L-1+NAA0～b′mg .L-1,或R +IBAc2 mg .L-1+NAAc1 mg .L-1生根率达 98%以上。移栽土壤基质以黄心土加沙(10∶1)理想 ,移栽成活率可达 85 %以上     

白玉兰离体培养和快速繁殖

试验建立了白玉兰离体繁殖体系。种子为外植体 ,初代培养基为 :B5 +BA0 .5mg/L +NAA0 .0 5mg/L + 30g/L糖 +琼脂 6g/L ,萌发率 85 %。继代培养基为 :B5 +BA1.5mg/L +NAA0 .15mg/L + 30g/glucose/sucrose + 6g/Lagar+Vco0 .5g/L ,月增殖率可达 5 .0± 0 .1。生根培养为 :1/2B5+NAA0 .5mg/L +AC1.0mg/L +VB2 0mg/L。以试管苗茎段、根段做外植体 ,培养基B5 +BA2mg/L+ 2 .4 -D0 .5mg/L +VC0 .5g/L ,可获得少量愈伤组织 ,继代存活率仅为 30 %。没有再分化成苗     

驱蚊香草的组织培养和快速繁殖

以驱蚊香草幼嫩枝条为外植体，进行离体培养，获得再生植株，通过这个过程，得出有利于芽诱导、增殖、诱导生根的最佳配方，为驱蚊香草快速繁殖，规模化生产打下技术基础。     

优良观赏保健树大叶冬青扩繁利用研究

在引进大叶冬青树种种质资源的基础上,系统地研究了大叶冬青生物学特性、生态适应性、生长习性、实生种播苗关键技术和组织培养及其微繁技术,以及生态复合拟生栽培技术,培育出了大叶冬青绿化苗木18万株。并测定了大叶冬青叶内可溶性蛋白质、超氧化物歧化酶(SOD)等抗衰老成分含量和叶组织中抗菌成分含量及其抑菌功能。因地制宜研制出优质嫩芽型、嫩叶炒青型、老叶微波干燥型3种苦丁保健绿茶,为大叶冬青叶资源产业化开发奠定了基础。     

FAST BREEDING OF GROUND-COVER CHRYSANTHEMUM

Ground-coverchrysanthemum(Dendranthemaxgrandof0raTzvel)isanexcellentpersistingrootflowerwithshortplant,highregressiveresistance,densebloomandcarelessmanagement.Itisre-gardedasagoodbreedforgardenaffores-tationandcomesintobloomaroundtheNationalDay.Cuttageandplantdivisionmethodsarethemostinuseforbreedingofthisspecies-Thetest-tubebreedingmeth-odwasad0ptedinthisexperimentinordertoincreasethebreedingc0efficientoftheimprovedbreed.MATERIALSANDMETHODSTheshort-yellowspeciesofground-coverchrysa…     

Comparison of image and rapid field assessments of riparian zone condition in Australian tropical savannas

Suitable methods for measuring and monitoring the condition of riparian environments are being investigated by government agencies responsible for maintaining these environments in Australia. The objective of this work was to compare two riparian condition assessment approaches, the Tropical Rapid Appraisal of Riparian Condition (TRARC) method developed for rapid on-ground assessment of the environmental condition of savanna riparian zones and an image based riparian condition monitoring scheme. Measurements derived from these two approaches were compared and correlated. The sample representativeness of the TRARC method was evaluated and the cost-effectiveness and suitability for multi-temporal analysis of the two approaches were assessed. Two high spatial resolution multi-spectral QuickBird satellite images captured in 2004 and 2005 and coincident field data covering sections of the Daly River in the Northern Territory, Australia were used in this work. Both field and image data were processed to map indicators of riparian zone condition including percentage canopy cover, organic litter on the ground, canopy continuity, tree clearing, bank stability, and flood damage. Spectral vegetation indices, image segmentation, and supervised classification were used to produce riparian health indicator maps. QuickBird image data were used to examine if the spatial distribution of TRARC transects provided a representative sample of ground based estimates of riparian health indicators. Covering approximately 3% of the study area, the sample mean of the TRARC estimates of individual indicators of riparian zone condition were in most cases within 20% of the global mean derived from the whole imaged riparian area. The cost-effectiveness of the image based approach was compared to that of the ground based TRARC method. Results showed that the TRARC method was more cost-effective at spatial scales from 1 km to 200 km of river in relatively homogeneous riparian zones along rivers with only one channel, while image based assessment becomes more feasible at regional scales (200–2000 km of river). A change detection analysis demonstrated that image data can provide detailed information on gradual change, while the TRARC method is less suited for multi-temporal analysis due to the ranked data format, which inhibits precise detection of change. However, results from both methods were considered to complement each other for single date assessment of riparian zones if used at appropriate spatial scales.     

朝仓花椒组织培养快繁试验

以朝仓花椒为试材，研究了其组培快繁技术。试验表明，4～5月份接种外植体易获得无菌苗；初期适宜的增殖培养基为MS＋6-BA1．0mg／L（单位下同）＋IBA0．2＋3．0％蔗糖，继代增殖培养基为MS＋6-BA0．5＋IBA0．1＋3．0％蔗糖，适宜的生根培养基为1／4MS＋NAA0．3＋2．5％蔗糖，移栽最佳基质为蛭石。