Q Sepharose FF分离藻红蛋白的工艺研究(英文)

[目的]建立Q Sepharose FF分离藻红蛋白的工艺方法并验证其放大可行性。[方法]通过对洗脱条件、上样体积以及洗脱流速的优化,确定最佳分离工艺条件;在确定的工艺条件下,将上样体积与柱体积等比例放大。[结果]Q Sepharose FF分离藻红蛋白的最佳工艺条件为:将30ml坛紫菜提取液加载到8ml Q Sepharose FF柱上,以1ml/min的流速使用添加0-0.10-0.35-1.00mol/LNaCl的NaH2PO4-Na2HPO4缓冲溶液(pH6.0)分步洗脱,收集0.35mol/LNaCl溶液洗脱时的色谱峰。在些条件下,R-藻红蛋白的回收率和纯度系数(A565/A280)分别为44.3和1.15。按照该工艺,将75ml藻红蛋白提取液加载到20ml Q Sepharose FF柱上进行分离,发现分离效果没有发生明显变化。[结论]使用Q Sepharose FF介质能够分离坛紫菜提取液中的藻红蛋白且该工艺易于放大。     

登陆强台风“凤凰”暴雨成因分析

利用常规观测资料、NCEP 1°×1°6 h再分析资料、NCEP/NCAR地表潜热通量一日4次再分析资料以及TBB资料等,对2008年第8号强台风＂凤凰＂登陆引起的暴雨进行了深入的机理分析。结果表明,充沛的水汽以及层结不稳定为暴雨的发生提供了有利的水汽和热力条件;高层有明显的辐散,低层有明显的辐合,且从低层到高层伴随着很强的垂直上升运动,为暴雨的发生提供了有利的动力条件;螺旋度的大值中心的演变基本反映了台风中心的移动方向,而强降水一般发生在正螺旋大值中心的右侧,且梯度大的一侧比梯度小的一侧降水强度更大;非地转湿Q矢量辐合中心对应未来12 h强降水的发生区,对判断台风在陆上的运动、维持也有一定的意义;地表潜热通量在此次暴雨过程的各个时段均为正值,其向上输送和强弱变化对台风强度的维持有一定的指示意义。     

牦牛Q—Tc和Q—Tr值的探讨

对７６头牦牛的Ｑ－Ｔｃ和Ｑ－Ｔｒ值研究表明，牦牛的Ｑ－Ｔｃ值具有年龄差异，成年牦牛，２岁牦牛和１－３月龄牦牛的Ｑ－Ｔｃ值分别为０．３１２，０．３２７和０．３４５。成年牦牛和２岁牦牛的Ｋ常数应定为０．３２ｓ，１－３月龄牦牛应定为０．３４ｓ。     

Antagonistic activity of endophytic fungi towards <Emphasis Type="Italic">Diplodia corticola</Emphasis> assessed by in vitro and in planta tests

One isolate each of Trichoderma viride, Epicoccum nigrum, Fusarium tricinctum, Alternaria alternata, Sclerotinia sclerotiorum and Cytospora (teleomorph: Valsa sp.) present in epigeous declining oak tissues was evaluated for its ability to control Diplodia corticola (isolate 79). This fungus is the causal agent of cankers, vascular necrosis and dieback on various oak species. Among the isolates tested, T. viride and F. tricinctum showed maximum in vitro inhibition of mycelial growth of D. corticola (isolate 79). Species were also evaluated for their ability to reduce mortality caused by D. corticola (isolate 79) of Quercus cerris and Q. pubescens seedlings under controlled conditions. Two series of inoculations were carried out through wounds in the stem; in the first, the distance between the point of inoculation of the antagonist and the pathogen was 6 cm, whereas in the second series the distance was shortened to 3 cm. In seedlings of Q. cerris and Q. pubescens at a distance of 3 cm, inoculation with F. tricinctum and A. alternata significantly reduced mortality caused by D. corticola (isolate 79). Inoculation of T. viride through artificial cuticular wounds in the stem of seedlings prevented the proliferation of D. corticola (isolate 79) only on seedlings of Q. cerris. All Q. pubescens seedlings treated with T. viride manifested pathological symptoms subsequent to proliferation of D. corticola (isolate 79). These observations indicate that the interactions between endophytes in planta and D. corticola (isolate 79) are complex and merit further study.     

苏州市引种美国栎属种源试验初报

苏州市郊和吴县市林场于 1999年 3月引进美国栎属 2个树种 ,8个种源 (包括柳叶栎 6个种源 ,红栎 2个种源 ) ,用青冈、麻栎和小叶栎为对照 ,进行培育大苗试验。经 1a观测 ,结果是 :柳叶栎的生长量和成活率均高于红栎。在柳叶栎种源中 ,以 9816 ,9818号生长量和成活率较高     

结实期水分亏缺对反义Wx基因转化系水稻籽粒淀粉合成关键酶活性及淀粉累积的影响

以反义Wx基因转化系水稻及其受体亲本武运粳7号(9522)为材料,研究了结实期水层灌溉和水分亏缺条件下籽粒淀粉合成关键酶活性、淀粉及直支链淀粉含量的变化。结果表明,以水层灌溉为对照,结实初期(花后5~10 d)的水分亏缺利于籽粒中腺苷二磷酸葡萄糖焦磷酸化酶(ADPGPase)和颗粒淀粉合成酶(GBSS)活性的提高,但随着水分亏缺时间的延长,到灌浆后期两种酶活性降低的速度要快于对照。水分亏缺显著降低了可溶性淀粉合成酶(SSS)的峰值。Q酶活性于水分胁迫下显著受到抑制,花后20 d到成熟期酶活性降低的幅度较大。而两类去分支酶(异淀粉酶和普鲁兰酶)于水分亏缺下活性显著升高,在灌浆后期花后25~30 d普鲁兰酶仍保持着较高的活性。与水层灌溉相比,水分亏缺下籽粒中总淀粉的积累和支链淀粉含量明显降低。GBSS最大活性与直链淀粉含量关系最为密切,呈极显著正相关（r=0.981**）,SSS、Q酶和DBE最大酶活性分别与淀粉和支链淀粉含量不显著相关性。由此推测土壤水分对支链淀粉合成和积累的影响并不是由某一种酶调节的,而是由SSS、Q酶和DBE以及其他一些酶共同催化完成的。     

对比中的升华——浅论现代曲剧《阿Q与孔乙己》的人物设置

现代曲剧《阿Q与孔乙己》,以独特的剧本构思和巧妙的人物设置取得了非常显著的成绩。剧作中,编剧创造性地把鲁迅不同作品中的人物融合到一个剧本中,呈现在一个舞台上。阿Q与孔乙己两个人物形象,在相互的对比与映衬之中深化和升华了原作人物形象的思想和意蕴。     

青海弧菌Q67冻干粉急性毒性测试方法研究

[目的]研究利用青海弧菌Q67冻干粉进行急性毒性测试的方法。[方法]通过一系列试验,探讨青海弧菌Q67冻干粉复苏及与毒物反应接触时间对毒性测试的影响,筛选出适宜青海弧菌急性毒性测试的毒性参照物,在充分借鉴国际标准ISO 11348的基础上分析现行发光抑制率计算方法的不足,提出了优化的计算方法。[结果]青海弧菌Q67冻干粉复苏的30～120 min内进行毒性测试,与毒物的反应时间控制在15～20 min时,测得数据稳定、可靠。ZnCl2适合用作青海弧菌生物急性毒性测试的毒性参照物。[结论]该研究为制定青海弧菌Q67冻干粉急性毒性测试标准提供理论依据。     

辅酶Q10应用的研究进展

辅酶Q10是一种脂溶性醌类化合物,在人体呼吸链中参与电子传递,具有促进细胞新陈代谢的功能。基于辅酶Q10的特点和作用,为进一步开发辅酶Q10,并对其进行有效的利用,以发挥其重要的价值,综述了辅酶Q10在医药卫生和美容护肤等领域中的应用及研究状况,并分析了辅酶Q10与其它药物之间的互作关系,并对其开发前景进行了展望。     

Quantitative Analysis of Influence Force of Shanghai Expo on GGDP

Quantitative study of the impact of Shanghai World Expo on green GDP （GGDP） is significant for deploying sustainable development policy in China. The formula of GGDP is： GGDP = GDP--the loss of natural resources environment relegation cost ＋ comprehensive utilized value of waste. Based on this, the study employed vector autoregressive （VAR） model to predict the value of GGDP and other economic variables on condition that there was no Shanghai World Expo. Then Influence of Expo is defined as the rate of increase of GGDP. The result demonstrated that Shanghai World Expo had accelerated the growth rate of GGDP vastly with the elimination of effect of Beijing Olympics. Additionally, the quantitative analysis between GGDP and other economic variables suggested GGDP would replace GDP to evaluate the development of economy. Finally, the paper proposed that incidents like World Expo can enhance the level of influence for a country and that post-impact of Shanghai Expo should continue to be used to promote GGDP and that GGDP should serve as one of the indicators in assessment of political achievement.