猪兰氏Q群链球菌病的诊断与防制

1998年7-8月间，江苏省苏北中部部分县市生猪发生一种急性、热性、败血性传染病，仅40余天内，该地区共有47个乡镇462个村8871个农户13472头生猪发病，死亡生猪6142头，经流行病学调查，临床症状和病理观察，病原分离、生化反应、动物试验及分离菌株送中国兽药监察所进行血清学鉴定，为兰氏Q群链球菌病。     

Effect of supplementation with the reduced form of coenzyme Q10 on semen quality and antioxidant status in dogs with poor semen quality: Three case studies

Oxidative stress owing to an imbalance between reactive oxygen species and antioxidants, such as coenzyme Q10 (CoQ10), is a major contributor to male infertility. We investigated the effects of the reduced form of CoQ10 (ubiquinol) supplementation on semen quality in dogs with poor semen quality. Three dogs received 100 mg of ubiquinol orally once daily for 12 weeks. Semen quality, serum testosterone, and seminal plasma superoxide dismutase (SOD) activity were examined at 2-week intervals from 2 weeks before ubiquinol supplementation to 4 weeks after the treatment. Ubiquinol improved sperm motility, reduced morphologically abnormal sperm, and increased seminal plasma SOD activity; however, it had no effect on testosterone level, semen volume, and sperm number. Ubiquinol supplementation could be used as a non-endocrine therapy for infertile dogs.     

短期模拟增温对杭州湾滨海湿地芦苇群落土壤呼吸速率的影响

采用原位OTCs模拟增温和LICOR-6400-09土壤呼吸室法,研究了杭州湾滨海湿地芦苇群落土壤呼吸及土壤酶活性对温度升高的响应.结果表明,通过短期的模拟增温,土壤呼吸速率与对照相比提高了17.36%;增温和对照的土壤呼吸速率日变化幅度分别为:3.23±0.29和2.75±0.68μmol/(m2.s);日动态变化呈单峰曲线,峰值出现在14:00左右,最低值在早上6:00;增温和对照下的土壤呼吸速率与10cm土壤温度呈极显著的指数相关性(p<0.01);与空气温度呈显著性指数相关性(p<0.05);增温和对照处理下Q10值分别为:3.42和2.74;在0～10cm土层,β-葡萄糖苷酶、蔗糖酶活性比对照有显著的提高(p>0.05),脲酶酶活性也有一定的提高(0.76%),碱性磷酸酶活性比对照略有降低(2.54%).     

An approach to clarifying public preferences about silvicultural systems: A case study concerning group selection and clear‐cutting

To avoid large clear‐cut areas in recreation areas, three experiments where group selection was used to transform even‐aged stands of Norway spruce (Picea abies (L.) Karst.) into uneven‐aged stands were started. Two of these group selection stands were compared with adjacent forests managed by the clear‐cut system on the basis of the opinion of seven voluntary groups. The respondents have given their judgement of the forest sites in a semantic differential test. All parties made assessments when visiting the forest sites. In addition, two of the groups made assessments of black and white photographs representing the forest sites. The methods used were found to be both reliable and valid. A very low correlation was obtained between the assessment of whole forest areas and the mean value of the assessments of the stands within the same area. According to this study, the group selection method is preferable to clear‐cutting in even‐aged stands of Norway spruce if the attitudes of the voluntary groups are to be met.     

Q热血清学检测方法的研究进展

Q热是由贝氏柯克斯体(Coxiella burnetii)引起的急性自然疫源性传染病。本病呈世界性流行,人类和动物普遍易感,已经成为当前分布最广的人兽共患病之一。文章综述了Q热的病原学、流行病学以及血清学检测方法的研究进展,为Q热的进一步研究与防控提供科学依据。     

Short-term Effects of Nitrogen Deposition on Soil Respiration of Cinnamomum camphora Plantation

The nitrogen deposition experiments on Cinnamomum camphora plantation of Hunan provincial botanical garden were simulated from June the 7th to October the 7th,2010,the nitrogen deposition levels was respectively as control(CK, 0 g·m^-2 a^-1),low nitrogen(LN,5 g·m^-2 a^-1),medium nitrogen(MN,15 g·m^-2 a^-1) and high nitrogen(HN, 30 g·m ^-2 a^-1).The soil respiration speed and soil surface temperature and the surface water content of the soil were determined by LI-8100 measuring instrument.The results show that the average values of soil respiration speed were CK (4.09±0.66μmol·m^-2 s^-1),LN(2.39±029μmol·m^-2 s^-1), MN(2.18±0.19μmol·m^-2s^-1),HN (2.28±0.25μmol·m^-2 s^-1),and the treatment of the CK was obviously higher than the other three treatments（P<0.01）.With different nitrogen concentrations deposition treatments,the Q₁₀ （temperature sensitivity coefficients） for soil respiration of CK,LN,MN and HN treatments were 1.84,1.71,1.83 and 1.56.It was positive correlation between the respiration speed and the surface（≤10 cm） temperature of tested soil.It was negative correlation between the respiration speed and the surface（≤5 cm） water content of tested soil（P>0.05）.Volumetric water content of 5-cm soil was between 0.266 6-0.294 4(m³·m^-3), and the monthly content did not vary too much during the research period（1.8%-9.4%）.The findings suggest that the initial stage of nitrogen deposition obviously influenced the soil respiration of C.camphora plantation.     

利木赞牛抑肌素基因Q204X及nt821突变快速检测方法

抑肌素(GDF8)是骨骼肌细胞生长的重要负调控因子。抑肌素基因突变是牛"双肌"性状形成的分子遗传基础,在不同牛品种中存在着不同的抑肌素基因突变类型,在利木赞品种中,"双肌"性状主要是抑肌素基因第2外显子的Q204X型突变及第3外显子nt821突变。本研究报道一种新的检测该突变的简便方法。应用突变型与正常型引物在一次PCR反应中同时检测出不同的等位基因。对Q204X突变型PCR产物克隆测序分析表明,该片段确实含有一个能导致抑肌素功能缺陷的Q204X突变。     

永顺落叶木莲开花与结实物候学研究

首次报道永顺落叶木莲开花与结实物候学特征。其花期在3月中旬至5月上旬,果期在4月中旬至10月上旬。其间有3次花周期和3次果实发育期,每次花周期和果实发育期天数不同。低海拔比高海拔开花早,落花期亦早,开花周期较短;低海拔较高海拔的果发育期长,种子散落期晚,果实最佳采集期在9月20日至9月30日。     

东、黄海主要鱼类Q/B值与种群摄食量研究

利用东、黄海渔业资源调查所获得的数据,在测定年栖息平均水温、渐进体重、尾鳍外形比以及胃含物分析基础上,根据经验公式计算得出了东、黄海103种鱼类的Q/B值。Q/B值总体范围从3.09(黄鱼安鱼康)到19.13(七星底灯鱼),平均值为7.97。根据Q/B值和生物量评估了鱼类种群年摄食量。黄海的重要鱼类(鳀鱼、竹荚鱼、鲐鱼、细纹狮子鱼和小黄鱼),共消耗了449万t/年。东海的重要鱼类(竹荚鱼、带鱼、发光鲷、鳀鱼和蓝点马鲛),共消耗了175万t/年。97种鱼类在黄海共摄食约466万t/年,在东海共摄食278万t/年。同时对东、黄海主要饵料生物(磷虾类、鳀鱼、发光鲷、七星底灯鱼、细条天竺鲷、日本枪乌贼、桡足类、樱虾类、细螯虾和脊尾腹褐虾)被摄食量进行了估算。主要饵料生物在这两个海区共被消耗了555万t/年,占整个97种鱼类摄食消耗的74.6%。     

Characterization of the Q.Ymym region on wheat chromosome 2D associated with wheat yellow mosaic virus resistance

Yellow mosaic disease, caused by wheat yellow mosaic virus (WYMV), is one of the most serious diseases of winter wheat in Japan and China. A single major QTL for WYMV resistance in the Japanese wheat variety 'Yumechikara', designated Q.Ymym, has been mapped on a 43.6 cM linkage block between the two markers Xcfd233 and Xgwm349 on chromosome 2D. We were able to obtain two recombinants within the block, which facilitated reducing the size of the linkage block. The pseudomolecule sequence of 'Chinese Spring' (CS) indicated that the original Q.Ymym region of 43.6 cM corresponded to 68.5 Mb and the narrowed Q.Ymym region represents a size of 27.3 Mb. The sequence features of the Q.Ymym region were unique in comparison with CS sequences, which may have led to the low recombination rate within the block. The Q.Ymym haplotype block was detected in other WYMV-resistant varieties but not in the susceptible varieties used in this study. The unique sequence structure of the Q.Ymym region allowed the development of co-dominant markers for use in marker-assisted selection.