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【研究目的】本文研究了淫羊藿苷对鸡胚成骨细胞增殖和分化的影响。【方法】将不同浓度的淫羊藿苷加入第二代成骨细胞中,分别于培养24、48、72h时,使用MTT法和PNPP法检测成骨细胞的增殖率和碱性磷酸酶(ALP)活性。【结果】淫羊藿苷促进鸡胚成骨细胞增殖和分化,但增殖作用不显著。其中当淫羊藿苷浓度为20ng/ml时,对成骨细胞增殖分化作用最强。【结论】淫羊藿苷能够显著促进鸡胚成骨细胞分化,对成骨细胞增殖有一定作用。 相似文献
93.
AIM: To investigate the effects of Centella asiatica extracts on the cell cycle and intracellular free calcium ( i) in spleen lymphocytes. METHODS: Centella asiatica was extracted with alcohol and different organic solvents. Sephadex-LH20 gel column chromatography was applied for further purification. To screen the active constituents, the scavenging ability of the fractions generated by elution of chromatography against radicals of OH·, DPPH· and their anti-proliferative effects on spleen lymphocytes were measured. To assess the cell cycle and i in spleen lymphocytes, flow cytometry was performed. RESULTS: The D fraction eluted from the crude extract of Centella asiatica was obtained, which had strong suppressive effect on lymphocyte proliferation and decreased the i of the cells in a dose-dependent manner. The cell cycle of the spleen lymphocytes arrested at S and G2/M stages when treated with the D fraction. CONCLUSION: The effects of Centella asiatica for lowing i and arresting cell cycle at S and G2/M stages of spleen lymphocytes are responsible for its anti-proliferative activitiy. 相似文献
94.
[目的]寻求植物生长调节物质对腋芽分化增殖的最佳配比。[方法]将旗帜和福星2个一品红品种的腋芽茎段接种于附加不同浓度和配比激素的培养基上,研究不同植物生长调节物质及配比对腋芽分化增殖的影响。[结果]培养基单独添加0.5~1.0mg/L6-BA时,一品红有较高的芽增殖率,且分化出的不定芽可正常生长发育成小苗。培养基单独添加0.1~0.5mg/LNAA对腋芽增殖率及苗高的影响差异并不明显,增殖率较低,但可促进不定芽发育健壮。旗帜以1.0mg/L6-BA与0.2mg/LNAA配比效果较好,有较高的芽增殖率,且芽生长健壮,培养40d后,苗高可达4cm。福星以0.5mg/L6-BA与0.1mg/LIBA配比效果最好,芽增殖率达4.8倍,且芽生长健壮,叶色绿。[结论]6-BA对一品红腋芽的分化和增殖起决定作用,可以诱导腋芽分化并增殖,与IBA、NAA配比效果更佳。NAA对不定芽的生长有明显的促进作用。 相似文献
95.
96.
马尾松毛虫暴发年份的自然种群生命表研究 总被引:8,自引:0,他引:8
通过组建自然种群生命表来研究暴发年份马尾松毛虫(Dendrolimus punctatus Walker)的种群动态。研究结果表明,在增殖期,马尾松毛虫的存活率达4.27%,种群趋势指数为7.6;在暴发初期马尾松毛虫的存活率为4.25%,种群趋势指数为4.81,增殖期易引起马尾松毛虫种群数量的剧增。这一结果表明,防治马尾松毛虫的关键时期在增殖期末期。 相似文献
97.
[目的]筛选"蓝丰"蓝莓增殖的最优方法,以降低成本,提高增殖系数。[方法]以蓝莓品种"蓝丰"为试验材料,研究了不同激素、不同草木灰含量及不同光质对"蓝丰"蓝莓芽诱导及增殖的影响。[结果]不同激素、不同草木灰含量及不同光质对蓝莓增殖均有影响;"蓝丰"最适的诱导培养基为WPM+ZT 2.0 mg/L;"蓝丰"蓝莓最适的增殖培养基为WPM+ZT 1 mg/L+NAA 0.08 mg/L+草木灰1.0g/L;"蓝丰"蓝莓组培快繁以红光处理增殖效果最好。[结论]建立了"蓝丰"蓝莓组培快繁体系,为其大规模生产提供理论依据。 相似文献
98.
J. W. Gunnink 《The Veterinary quarterly》2013,33(2):52-54
Summary An investigation concerning the association of the leucocytecotyledon reaction and the expulsion of the placenta is described. The in vitro reaction does not appear to be individual‐specific. It is thus possible to determine the activity of the leucocytes against a cotyledon before parturition. The leucocytes of cows which will develop a retained placenta have lowered activity against the chemotactic stimulus of a cotyledon; this reduced activity of the leucocytes can be observed some days before parturition. 相似文献
99.
Tammi L Neville Allison M Meyer Arshi Reyaz Pawel B Borowicz Dale A Redmer Lawrence P Reynolds Joel S Caton Kimberly A Vonnahme 《畜牧与生物技术杂志(英文版)》2013,4(1):6
Background
Objectives were to examine the effects of selenium (Se) supply and maternal nutritional plane during gestation on mammary gland growth, cellular proliferation, and vascularity at parturition and d 20 of lactation. Rambouillet primiparous ewes (n = 84) were allocated to treatments in a 2 x 3 factorial. Factors were dietary Se (adequate Se [ASe, 11.5 μg/kg BW] or high Se [HSe, 77.0 μg/kg BW]) and nutritional plane (60% [RES], 100% [CON], or 140% [EXC]). At parturition, lambs were removed and 42 ewes (7/treatment) were necropsied. Remaining ewes were fed a common diet meeting requirements for lactation and mechanically milked twice daily until necropsy on d 20. At both necropsy periods, mammary glands were dissected and tissues harvested. Samples were analyzed for RNA, DNA, and protein content, cell proliferation, and vascularity. Where interactions were present (P ≤ 0.05), least squares means from the highest-order interaction are presented.Results
Final body weight of ewes was least (P ≤ 0.002) in RES, intermediate for CON, and greatest for EXC, regardless of stage of the ewe at necropsy (parturition or d 20 of lactation). In ewes necropsied at parturition, mammary glands were heavier (P = 0.02) in EXC compared to RES, with CON intermediate. Concentration of RNA (mg/g) was decreased (P = 0.01) in EXC compared to CON at parturition. There was a tendency (P = 0.07) for a Se by nutrition interaction in percentage of cells proliferating where ASe-EXC ewes had greater (P ≤ 0.02) number of proliferating cells then all other treatments. Mammary vascular area tended (P = 0.08) to be affected by a Se by nutrition interaction where ASe-CON had less (P = 0.007) vascular area than HSe-CON ewes. In ewes necropsied at d 20 of lactation, the number of alveoli per area was decreased (P ≤ 0.05) in RES compared to CON and EXC-fed ewes.Conclusions
Results of this study indicate that proper maternal nutritional plane during gestation is important for mammary gland development, even out to d 20 of lactation. 相似文献100.