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Tammi L Neville Allison M Meyer Arshi Reyaz Pawel B Borowicz Dale A Redmer Lawrence P Reynolds Joel S Caton Kimberly A Vonnahme 《畜牧与生物技术杂志(英文版)》2013,(4):287-293
Background: Objectives were to examine the effects of selenium (Se) supply and maternal nutritional plane during gestation on mammary gland growth, cellular proliferation, and vascularity at parturition and d 20 of lactation. Rambouillet primiparous ewes (n = 84) were allocated to treatments in a 2 x 3 factorial. Factors were dietary Se (adequate Se [ASe, 11.5 μg/kg BW] or high Se [HSe, 77.0 μg/kg BVV]) and nutritional plane (60% IRES], 100% [CON], or 140% [EXC]). At parturition, lambs were removed and 42 ewes (7/treatment) were necropsied. Remaining ewes were fed a common diet meeting requirements for lactation and mechanically milked twice daily until necropsy on d 20. At both necropsy periods, mammary glands were dissected and tissues harvested. Samples were analyzed for RNA, DNA, and protein content, cell proliferation, and vascularity. Where interactions were present (P 〈 0.05), least squares means from the highest-order interaction are presented. Results: Final body weight of ewes was least (P 〈 0.002) in RES, intermediate for CON, and greatest for EXC, regardless of stage of the ewe at necropsy (parturition or d 20 of lactation). In ewes necropsied at parturition, mammary glands were heavier (P = 0.02) in EXC compared to RES, with CON intermediate. Concentration of RNA (rag/g) was decreased (P= 0.01) in EXC compared to CON at parturition. There was a tendency (P= 0.07) for a Se by nutrition interaction in percentage of cells proliferating where ASe-EXC ewes had greater (P_〈 0.02) number of proliferating cells then all other treatments. Mammary vascular area tended (P = 0.08) to be affected by a Se by nutrition interaction where ASe-CON had less (P= 0.007) vascular area than HSe-CON ewes. In ewes necropsied at d 20 of lactation, the number of alveoli per area was decreased (P 〈- 0.05) in RES compared to CON and EXC-fed ewes. Conclusions: Results of this study indicate that proper maternal nutritional plane during gestation is important for mammary gland development, even out to d 20 of lactation. 相似文献
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根据目前丽格海棠组织培养快繁现状,研究了以MS培养基为基础附加不同种类和配比植物激素对丽格海棠组织培养中不定芽再生、不定芽继代增殖和生根的影响。结果表明:丽格海棠不定芽再生的最佳植物激素配比为MS+1mg/L6-BA+0.1mg/LTDZ或MS+1mg/L6-BA+0.1mg/L2.4-D;不定芽继代增殖的最佳植物激素配比为MS+2mg/L6-BA+0~0.1mg/LNAA;组培苗生根的最佳植物激素配比为MS+0.1~0.3mg/LIBA。 相似文献
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为建立‘红国王’葡萄组培快繁体系,本试验研究了生长素、接种材料类型、培养条件对组培苗继代增殖与生根的影响。结果表明,0.5 mg/L IAA适合‘红国王’的继代增殖,繁殖系数为5.49,在培养基中附加0.3 mg/L NAA,愈伤组织少且根系粗壮;适合‘红国王’组培苗生长的蔗糖浓度为25~30 g/L,在该浓度下植株长势健壮,生根条数多;带半叶单芽茎段为‘红国王’适宜接种材料类型;接种后第1天至第10天暗培养,可促进‘红国王’根系发育;放置于LED灯复合光红∶蓝∶白=3∶1∶1下,可获得长势强、根系强壮的组培苗;继代间隔为40 d时,繁殖速度较快。 相似文献
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Domínguez-Bernal G Tierrez A Bartolomé A Martínez-Pulgarín S Salguero FJ Antonio Orden J de la Fuente R 《Veterinary microbiology》2008,130(3-4):298-311
Live attenuated Salmonella enterica strains have been extensively studied as potential vectors for the oral delivery of heterologous antigens. Due to its ability to target immune cells, its specific mechanism for crossing the intestinal barrier, and its swine-restricted tropism, S. enterica subspecies enterica serovar Choleraesuis (S. Choleraesuis) has attracted a great deal of interest for the production of bacterial-based oral carriers specifically adapted to swine. In this study, two mutants of S. Choleraesuis were constructed and their attenuation and intracellular fate analysed with the purpose of engineering new attenuated live strains with improved properties as oral vaccine carriers. Those strains harboured a specific deletion either within the phoP or rpoS genes, which encode virulence-related regulators in S. Typhimurium. In comparison to the wild-type parental S. Choleraesuis, the mutant strains, especially DeltaphoP, were extremely low in virulence in the murine model and in the natural host, the pig. Moreover, when compared with a commercial live vaccine strain, SC-54, the two mutants showed a higher level of attenuation in mice and DeltaphoP also in pigs. In addition, DeltarpoS and DeltaphoP presented a proliferation and survival phenotype within swine intestinal primary fibroblast and macrophage cell cultures, respectively. Collectively, the present results indicate that the DeltarpoS and DeltaphoP strains of S. Choleraesuis gather adequate features to be potential candidates for vaccine vectors for the specific delivery of heterologous antigens adapted to pigs. 相似文献
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The aim of these in vitro experiments was (1) to examine the effects of ghrelin on the basic functions of ovarian cells (proliferation, apoptosis, secretory activity); (2) to determine the possible involvement of the GHS-R1a receptor and PKA- and MAPK-dependent post-receptor intracellular signalling cascades; (3) to identify the active part of the 28-amino acid molecule responsible for the effects of ghrelin on ovarian cells. We compared the effect of full-length ghrelin 1-28, a synthetic activator of GHS-R1a, GHRP6, and ghrelin molecular fragments 1-18 and 1-5 on cultured chicken ovarian cells. Indices of cell apoptosis (expression of the apoptotic peptide bax and the anti-apoptotic peptide bcl-2), proliferation (expression of proliferation-associated peptide PCNA), and expression of protein kinases (PKA and MAPK) within ovarian granulosa cells were analysed by immunocytochemistry. The secretion of progesterone (P(4)), testosterone (T), estradiol (E(2)) and arginine-vasotocin (AVT) by isolated ovarian follicular fragments was evaluated by RIA/EIA. It was observed that accumulation of bax was increased by ghrelin 1-28, GHRP6 and ghrelin 1-18, but not by ghrelin 1-5. Expression of bcl-2 was suppressed by addition of ghrelin 1-28, GHRP6 and ghrelin 1-5, but promoted by ghrelin 1-18. The occurrence of PCNA was reduced by ghrelin 1-28, GHRP6, ghrelin 1-18 and ghrelin 1-5. An increase in the expression of MAPK/ERK1, 2 was observed after addition of ghrelin 1-28, GHRP6 and ghrelin 1-18, but not ghrelin 1-5. The accumulation of PKA decreased after treatment with ghrelin 1-28 and increased after treatment with GHRP6 and ghrelin 1-18 but not ghrelin 1-5. Secretion of P(4) by ovarian follicular fragments was decreased after addition of ghrelin 1-28 or ghrelin 1-5 but stimulated by GHRP6 and ghrelin 1-18. Testosterone secretion was inhibited by ghrelins 1-28 and 1-18, but not by GHRP6 or ghrelin 1-5. Estradiol secretion was reduced after treatment with ghrelin 1-28 but stimulated by ghrelins 1-18 and 1-5; GHRP6 had no effect. AVT secretion was stimulated by ghrelin 1-28, GHRP6 and ghrelin 1-18, but inhibited by ghrelin 1-5. The comparison of the effects of the four ghrelin analogues on nine parameters of ovarian cells suggest (1) a direct effect of ghrelin on basic ovarian functions-apoptosis, proliferation, steroid and peptide hormone secretion; (2) that the majority of these effects can be mediated through GHS-R1a receptors; (3) an effect of ghrelin on MAPK- and PKA-dependent intracellular mechanisms, which can potentially mediate the action of ghrelin at the post-receptor level; (4) that ghrelin residues 5-18 may be responsible for the major effects of ghrelin on the avian ovary. 相似文献
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水稻胚乳细胞增殖和充实与子房生理活性变化关系的研究 总被引:3,自引:1,他引:2
结合水稻不同粒位籽粒的胚乳增殖动态和单细胞充实状况,分析了胚乳细胞增殖过程中子房若干生理指标的变化特点,结果表明:籽粒呼吸量较大的时期为胚乳细胞物质充实期,而单位鲜重呼吸强度最大在胚乳细胞分裂期,弱势粒呼吸强度在花后有一低水平期,随着细胞分裂的起动开始上升;胚乳细胞分裂期保持有较物质充实期高得多的含水率;子房ATP水平在胚乳细胞分裂期处于较高水平;可溶性糖含量强势粒在胚乳细胞分裂期水平较高,至细胞 相似文献
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猪外周血单个核细胞增殖反应影响条件的探讨 总被引:8,自引:0,他引:8
应用 L16 (45) 正交试验, 对影响猪 P B M C 增殖反应的几个因素, 包括培养时间、 P H A 浓度、细胞浓度等三个因素四个水平进行了比较和探索。试验表明几个因素对 P B M C 的增殖反应均有显著的影响( P< 005) , P B M C 增殖反应的 M T T 比色法的最佳反应条件组合为 P B M C 浓度为1 ×106/ml, 植物血凝素 P H A 的浓度为125μg/ ml, 细胞培养时间为24 小时; 影响增殖反应的先后顺序为 P B M C 浓度、 P H Ap 浓度及细胞培养时间。同时确定了最有利于 P B M C 增殖的犊牛血清浓度为10 % 。 相似文献
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