对草地贪夜蛾高毒力的苏云金芽胞杆菌菌株筛选与杀虫活性研究

草地贪夜蛾是一种世界性重大农业害虫,给玉米等多种主要粮食和经济作物造成严重危害.为实现对草地贪夜蛾的高效、绿色、持续防控,亟需筛选高毒力苏云金芽胞杆菌菌株资源.本研究以前期实验室储备的对斜纹夜蛾、小地老虎具有杀虫活性的363株野生菌株库为候选菌株,基于菌株杀虫基因差异及进化关系,去除冗余菌株后获得172株候选菌株.通过...     

非1B/1R类型小麦雄性不育系KTSP3314A败育的生化机制

为了揭示K型非1B/1R类型小麦不育系雄性败育的生化机制。利用K型非1B/1R类型小麦雄性不育系KTSP3314A,其同型保持系TSP3314B,以K型1B/1R类型小麦雄性不育系K3314A、其同型保持系3314B为对照,分别对其叶片和穗子在不同发育时期的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性和丙二醛(MDA)含量的变化进行测定。结果表明,K型非1B/1R类型小麦不育系中SOD,POD及CAT活性变化与K型1B/1R类型小麦雄性不育系酶活性变化一致,K型非1B/1R类型雄性不育小麦叶片和穗子的超氧化物歧化酶(SOD)后期则较低,过氧化氢酶(CAT)、过氧化物酶(POD)的活性后期较高,丙二醛(MDA)含量后期较高。说明二者的雄性败育生化机制基本一致,但前者比后者败育的时期较晚。且超氧化物歧化酶(SOD)活性与雄性育性有关。     

盐胁迫下诱抗剂对水稻P450基因差异表达的分析

以盐胁迫为对照,诱抗剂处理水稻R6幼苗根系后,采用Oligo芯片技术筛选和分析了P450基因的差异表达;进一步以H2O和盐胁迫为对照,诱抗剂处理水稻R6幼苗根系后,利用半定量RT-PCR检测P450基因的差异表达,结果表明,P450基因OsHI-1在盐胁迫下特异表达,在诱抗剂HI的作用下增强表达。     

Identification of Ligands for Chicken Transient Receptor Potential Ankyrin 1 Channel and Chemosensory Perception of Herbal Compounds in Chickens

The pungency induced by spices and herbs plays an important role in food choice and appetite, and it is suggested that adding spices and herbs to feed as natural alternatives to antibiotics has beneficial effects in poultry farming. However, our knowledge of the chemosensory perception of herbal compounds in chickens is limited. Transient receptor potential ankyrin 1 (TRPA1) is involved in the sensory perception of various herbal compounds. Here, we performed calcium imaging and electrophysiological analyses using cells transiently expressing chicken TRPA1 (cTRPA1) and identified two novel cTRPA1 ligands—eugenol and thymol. In a behavioral assay, chickens responded to cTRPA1 ligands, including eugenol, thymol, cinnamaldehyde, carvacrol, and allyl isothiocyanate. These results provide evidence that chickens have a functional TRPA1 channel and chemosensory perception of various herbal compounds.     

不同类型有机物料的有机磷组成及生物有效性

[目的]研究不同有机物料的有机磷组成及其作为磷源施用后的供磷能力,为化肥磷的有机替代奠定理论基础.[方法]供试有机物料包括粪肥类(猪粪、羊粪)、绿肥类(豌豆、苜蓿和绿豆)、秸秆类(小麦秸秆、玉米秸秆和油菜秸秆).分析了8种有机物料的全磷、有机磷含量和C/P值,采用Bowman-Cole方法测定了有机磷中的活性(LOP)...     

Dissecting the genetic basis of maize deep-sowing tolerance by combining association mapping and gene expression analysis

Deep-sowing is an important method for avoiding drought stress in crop species,including maize.Identifying candidate genes is the groundwork for investigating the molecular mechanism underlying maize deep-sowing tolerance.This study evaluated four traits(mesocotyl length at 10 and 20 cm planting depths and seedling emergence rate on days 6 and 12) related to deep-sowing tolerance using a large maize population containing 386 inbred lines genotyped with 0.5 million high-quality single nucleotide ...     

高温蘑菇浙AgH—1种型的鉴定

本文描述了高温蘑菇浙ＡｇＨ－１的形态学和生物学特性,该菌株最适出菇温度为２７￣３０℃,子实体能在３５￣３８℃下生长。此外该菌株子实体的口感和风味明显优于以往文献报道的双环蘑菇,因此该菌株是双环蘑菇的一个新的生理型。     

辣椒新品种湘椒6号的选育

湘椒６号是由湖南地方良种伏地尖中选出的自交系Ｆ８１－１－２从北方引进的甜椒品种经６代单株选择而成Ｌ２４－１自交系配制而成的一代杂各。该品种植株生长势强,耐寒、耐湿,果实粗牛角形,果色深绿,微辣,商品性好。从定值致收获３５￣４５天,６６７ｍ＾２产鲜椒３０００ｋｇ。田间表现抗病毒病、疮痂病。     

Effects of oxidized high-density lipoprotein on membrane fluidity and the expression of lymphocyte function associated antigen-1 of monocyte

AIM: To investigate the effects of oxidized high-density lipoprotein (oxHDL) on membrane fluidity and the expression of lymphocyte function associated antigen(LFA-1) of monocyte. METHODS: The membrane fluidity of THP-1 cells was assayed by fluorescence anisotropy with DPH (1,6-dipheny-1,3,5-hexatriene), a fluorescent probe; The LFA-1 expression on THP-1 cells were assayed by flow cytometry with indirect immunofluorescence.RESULTS: The membrane fluidity of THP-1 cells was reduced by 45% and 52% respectively (P＜0.01) after incubation with 100 μg protein/mL oxHDL and oxLDL for 20 h; oxHDL could stimulated the expression of LFA-1 on THP-1 cells, the positive cell rate and mean fluoresence intensity (MFI) increased by 39% and 45% respectively versus control group (P＜0.01) after incubation with cells for 24 h. CONCLUSIONS: By increasing the expression of LFA-1 on monocyte, oxHDL can promote the monocyte-endothelium adhesion; oxHDL also can reduce the membrane fluidity of monocyte, that will limit the returning of monocytes from subendothelium back to the circulation. This suggested that oxHDL may play an important role in atherogenesis.     

Protective effect of C1q/tumor necrosis factor-related protein 3 on vascular endothelium in rats with hyperuricemia

AIM To study whether C1q/tumor necrosis factor （TNF）-related protein 3 （CTRP3）protect vascular endothelium in rats with hyperuricemia and its potential mechanisms. METHODS An animal model of hyperuricemia was established by using male SD rats drinking 10% fructose water （n=10）. The rats drinking normal water served as normal controls （n=10）. After 12 weeks, the rats were given a single injection with Ad-CTRP3 or Ad-GFP. The experiment was ended at 14th day after transfection.The serum levels of uric acid and nitric oxide （NO） were evaluated. The serum contents of TNF-α and interleukin-6 （IL-6） were measured by ELISA. HE staining and TUNEL assay were used to assess the morphological changes of intima and apoptosis of endothelial cells in thoracic aorta, respectively. The mRNA levels of endothelial nitric oxide synthase （eNOS）, TNF-α and IL-6 were detected by RT-qPCR. The protein levels of CTRP3 and Toll-like receptor 4 （TLR4） were determined by Western blot. RESULTS Compared with normal control group, the rats with hyperuricemia showed lower CTRP3 and higher TLR4 protein levels in the thoracic aorta （P<0.05）. Hyperuricemic rats had higher serum contents of uric acid, TNF-α and IL-6 （P<0.05）. Also, the intima structure disturbance of thoracic aorta, increased apoptotic rate, higher mRNA levels of TNF-α and IL-6 as well as lower mRNA levels of eNOS were observed （P<0.05）. By contrast, CTRP3 over-expression decreased TLR4 protein levels, reduced inflammatory cytokines, and obviously improved the morphology and function of thoracic aorta in the rats with hyperuricemia. CONCLUSION CTRP3 protect vascular endothelium in rats with hyperuricemia maybe via down-regulation of TLR4- mediated inflammatory signaling pathway.