Effects of genes increasing the number of spikelets per panicle,TAW1 and APO1, on yield and yield-related traits in rice

The genes TAWAWA1 (TAW1) and ABERRANT PANICLE ORGANIZATION1 (APO1) increase the number of spikelets per panicle (SN). In the present study, we examined the effects of these genes on morphological traits, yield, and yield-related traits including yield components using the near-isogenic lines (NILs) in the genetic background of a japonica rice variety, Koshihikari – NIL-taw1, NIL-apo1-D3, and NIL-apo1-D4 – in a field experiment. The SN and total number of spikelets per area of the three NILs were larger than those of Koshihikari. However, the yield of the three NILs did not exceed that of Koshihikari due to their low filling ability. Interestingly, our field experiments indicated that TAW1 did not affect the diameter of internodes and the PN, whereas APO1 decreased the PN and increased the diameter of internodes. These results suggest that TAW1 and APO1 differently affect yield-related traits.     

Development of a kompetitive allele‐specific PCR marker for selection of the mutated Wx‐D1d allele in wheat breeding

Waxy (Wx) protein is a key enzyme for synthesis of amylose in endosperm. Amylose content in wheat grain influences the quality of end‐use products. Seven alleles have been described at the Wx‐D1 locus, but only two of them (Wx‐D1b, Wx‐D1e) were genotyped with codominant markers. The waxy wheat line K107Wx1 developed by treating ‘Kanto 107’ seeds with ethyl methanesulphonate carries the Wx‐D1d allele. However, no molecular basis supports this nomenclature. In the present study, DNA sequence analysis confirmed that a single nucleotide polymorphism in the sixth exon of Wx‐D1 changed tryptophan at position 301 into a termination codon. Based on this sequence variation, a PCR‐based KASP marker was developed to detect this point mutation using 68 BC₈F₁ plants and 297 BC₈F₂ lines derived from the cross ‘Ningmai 14’*9/K107Wx1. Combined with codominant markers for the Wx‐A1 and Wx‐B1 alleles, waxy and non‐waxy near‐isogenic lines were distinguished. The KASP marker was efficient in identifying the mutant allele and can be used to transfer waxiness to elite lines.     

水稻紫鞘染色体片段代换系Z519的鉴定及PSH1候选基因分析

花青素是广受人们喜爱的植物色素,在食品加工、杂种纯度鉴定中具有重要的作用。本研究鉴定了一个以日本晴为受体、优良紫鞘恢复系R225为供体亲本的紫鞘水稻染色体片段代换系Z519。Z519共含有16个代换片段,分布于除第10染色体外的其他11条染色体,平均长度为6.85 Mb。Z519在芽鞘3 mm时鞘尖呈现紫色,其后在叶鞘、叶缘、茎维管束和柱头等部位出现紫色线条,而日本晴各部位均为绿色。Z519叶鞘中花青素含量极显著高于日本晴,剑叶中没有显著差异。与受体日本晴相比,Z519的株高显著降低,千粒重、主穗总粒数和实粒数显著增加,有效穗数、主穗长和结实率无显著差异。进一步以日本晴与Z519杂交产生的F1和F2群体对紫鞘基因进行了遗传分析和分子定位。该紫鞘表型受显性单基因控制,位于第1染色体In Del标记L03和SSR标记L01之间37.8 kb的区域,被命名为PSH1。对该区间进行候选基因预测和测序,Z519在一个编码质体ATP/ADP转运蛋白的LOC_Os01g45910基因第一外显子的第238~252碱基的GTG重复区又多插入了GTG 3个碱基,导致增加了一个甘氨酸。q RT-PCR结果进一步表明其表达量在Z519中明显降低,初步确定LOC_Os01g45910是PSH1的候选基因。该研究为PSH1调控花青素的分子机制奠定了良好基础。     

水、旱条件下小麦苗期部分农艺性状的遗传分析

为研究水分胁迫对小麦幼苗部分农艺性状的影响,分析这些性状与干旱胁迫的关系。以‘青麦6号’和‘烟农24’杂交形成的F2:3群体为材料,采用主基因+多基因混合遗传模型对其进行遗传分析。水、旱2种条件下,根长和根鲜重均为1对加性-显性效应主基因模型;茎鲜重、茎干重、根干重、根茎鲜重比和根茎干重比的最适遗传模型不同。干旱胁迫条件下,茎鲜重主基因遗传率为37%,茎干重、根长、根鲜重、根干重、根茎鲜重比及根茎干重比主基因遗传率分别是51%、81%、74%、56%、50%、68%。茎鲜重、茎干重、根干重等性状,尽管检测到主基因,但在水旱条件下遗传模型不同,可进一步研究其遗传规律。     

拉枝角度对‘SH1’矮化中间砧‘富士’苹果幼树生长和结果的影响

旨在为‘SH1’矮砧‘富士’提供幼树树形管理的科学依据。本研究以4年生‘SH1’矮砧‘富士’为试材,通过70°、90°、120°3种拉枝角度的处理,探讨不同拉枝角度对苹果幼树生长和结果的影响。结果表明:拉枝角度120°时叶片光合速率最大,且与其他2个处理差异显著(P0.05);拉枝角度90°时叶绿素a、叶绿素b和总叶绿素含量最高、叶片厚度、百叶重最大,与其他2个处理差异显著(P0.05);拉枝角度90°时的冠下光合间接辐射最大,拉枝角度120°时株间光合间接辐射、光合直接辐射和叶面积指数均最大;拉枝角度90°时长枝的长度、粗度最大,中枝粗度最大,拉枝角度120°时中枝长度最长;拉枝角度90°时果实单果重、果形指数、可溶性固形物含量、硬度均最大。综合考虑树体生长势和果实品质等因素,90°为‘SH1’矮砧‘富士’枝条适宜的拉枝角度。     

不同浓度1-MCP对早酥梨果实常温贮藏品质的影响

以早酥梨为试材,研究常温(20℃),相对湿度80%~90%贮藏条件下不同浓度(0.5、1.0、1.5μL/L)的1-MCP对早酥梨果实贮藏品质的影响。结果表明,1-MCP处理能够较好地抑制果实叶绿素含量的降低与黄化指数的升高,延缓早酥梨果实的黄化、软化过程,并保持果实较高的可溶性固形物和可滴定酸含量。其中,1.5μL/L浓度的1-MCP对早酥梨果实的黄化控制效果最好,且能较好地保持果实品质,是早酥梨果实的适宜处理浓度。     

基于GM(1,1)的天然次生林空间结构预测

【目的】系统地分析已有天然次生林林分空间结构数据,通过灰色模型预测天然次生林林分空间结构各指标未来的发展趋势,对天然林经营具有十分重要的意义。【方法】以湖南大围山自然保护区典型次生林为研究对象,依据结构化森林经营理论,选取了混交度、竞争指数、角尺度、空间密度指数、开阔比数、大小比数、林分综合均质性指数作为天然次生林林分空间结构合理性评价与预测的量化指标,构建了基于GM(1,1)的天然次生林林分空间结构灰色预测模型。模型将2008年林分空间结构各指标的平均值作为初始值,并在研究区设置了面积为20 m×20 m的5个研究样地(M1,M2,M3,M4,M5),利用保护区2008-2018年典型样地林分空间信息,预测了研究区调研样地2019年-2021年林分空间结构各指标未来的变化趋势。【结果】利用精度检验机制对该模型的精确度进行了有效性检验,检验结果表明,所有指标预测合格概率P合=71.43%,良好的概率P良好=22.86%,优的概率P优=5.71%,表明该预测模型符合精度检验要求。【结论】样地未来3 a各指标整体变化尺度不大,林分空间结构基本稳定。从各指标在2008-2018年时空上的变化规律来看,各样地林分平均竞争指数、平均大小比数及平均空间密度指数是影响林分均质性指数的关键指标。     

便携式木材绞盘机新型卷筒离合器设计

在利用便携式木材绞盘机进行木材生产试验过程中,发现卷筒离合器在分离与接合过程中易出现卡阻现象,从而影响集材生产效率。在对牙嵌式离合器结构进行受力分析和卡阻原因分析的基础上,基于Solid Works的建模分析设计方法,设计了一种新型多孔旋转圆柱式卷筒离合器,该方法有效缩短了新型卷筒离合器的设计周期。新型卷筒离合器采用圆柱销与圆孔的嵌合,代替牙嵌式离合器牙嵌轴与牙嵌套的嵌合,同等试验条件下,旋转柱销式卷筒离合器平均分离时间分别比柱销式卷筒离合器节省3.71 s、比牙嵌式卷筒离合器节省8 s,改善了便携式木材绞盘机卡阻后离合器分离困难的现象,节约了离合器分离时间,从而有效提高了便携式木材绞盘机在集材作业中的生产效率。     

生物质导热系数测量系统设计

随着不可再生能源的日渐枯竭,生物质能源的研究工作受到越来越多的关注。想要充分了解和利用生物质能源,生物质材料的热物性分析必不可少。其中,导热系数表征就是研究的重点内容之一,设计出可靠便捷的测量系统至关重要。笔者以LabVIEW平台为基础,依靠其强大的测控功能,结合3ω法测量原理及具体的实验步骤,设计出一种固液相样品都适用的导热系数测量系统。实验中测量端的铂丝将产生电信号,经前置放大电路被锁相放大器SR830采集,然后通过GPIB接口卡与PC机通讯。其中,上位机LabVIEW的程控交互界面包括了前期准备、信息记录、数据采集和曲线显示4个部分,为研究人员提供了直观、便捷、高效的实验进程帮助。还从测量对象的性质和实验台搭建过程两个方面,分析了可能存在的误差来源。为了验证该导热系数测量系统的可靠性,对常见的生物质液体,包括存在固液相变化的样品,进行了测量实验。测量值与文献参考值比较显示,系统误差小于5%,说明该测量系统具有较高的可靠性和稳定性。     

Intensive organic vegetable production increases soil organic carbon but with a lower carbon conversion efficiency than integrated management

Intensive vegetable production in greenhouses has rapidly expanded in China since the 1990s and increased to 1.3 million ha of farmland by 2016, which is the highest in the world. We conducted an 11‐year greenhouse vegetable production experiment from 2002 to 2013 to observe soil organic carbon (SOC) dynamics under three management systems, i.e., conventional (CON), integrated (ING), and intensive organic (ORG) farming. Soil samples (0–20 and 20–40 cm depth) were collected in 2002 and 2013 and separated into four particle‐size fractions, i.e., coarse sand (> 250 µm), fine sand (250–53 µm), silt (53–2 µm), and clay (< 2 µm). The SOC contents and δ¹³C values of the whole soil and the four particle‐size fractions were analyzed. After 11 years of vegetable farming, ORG and ING significantly increased SOC stocks (0–20 cm) by 4008 ± 36.6 and 2880 ± 365 kg C ha^?1 y^?1, respectively, 8.1‐ and 5.8‐times that of CON (494 ± 42.6 kg C ha^?1 y^?1). The SOC stock increase in ORG at 20–40 cm depth was 245 ± 66.4 kg C ha^?1 y^?1, significantly higher than in ING (66 ± 13.4 kg C ha^?1 y^?1) and CON (109 ± 44.8 kg C ha^?1 y^?1). Analyses of ¹³C revealed a significant increase in newly produced SOC in both soil layers in ORG. However, the carbon conversion efficiency (CE: increased organic carbon in soil divided by organic carbon input) was lower in ORG (14.4%–21.7%) than in ING (18.2%–27.4%). Among the four particle‐sizes in the 0–20 cm layer, the silt fraction exhibited the largest proportion of increase in SOC content (57.8% and 55.4% of the SOC increase in ORG and ING, respectively). A similar trend was detected in the 20–40 cm soil layer. Over all, intensive organic (ORG) vegetable production increases soil organic carbon but with a lower carbon conversion efficiency than integrated (ING) management.