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971.
Paula Martins-Lopes José Lima-Brito Sónia Gomes Julieta Meirinhos Luís Santos Henrique Guedes-Pinto 《Genetic Resources and Crop Evolution》2007,54(1):117-128
Thirty Portuguese and eight foreign olive (Olea europaea L.) cultivars were screened using Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR) markers.
Twenty RAPD primers amplified 301 reproducible bands of which 262 were polymorphic; and 17 ISSR primers amplified 204 bands
of which 180 were polymorphic. The percentage of polymorphic bands detected by ISSR and RAPD was similar (88 and 87%, respectively).
The genetic variability observed was similar in the Portuguese and foreign olive cultivars. Seven ISSR and 12 RAPD primers
were able to distinguish individually all 38 olive cultivars. Twenty specific molecular markers are now available to be converted
into Sequence Characterised Amplified Region (SCAR) markers. Relationships among Portuguese and foreign cultivars is discussed. 相似文献
972.
Whole cell fatty acid (WCFA) compositions of three different structures of ectomycorrhizal (ECM) fungi: sporocarps, pure culture mycelia and ectomycorrhizas were analysed to evaluate the potential use of fatty acid profiles as biomarkers for ECM fungi and ectomycorrhiza-associated bacteria. Sporocarps of Amanita muscaria, Amanita rubescens, Lactarius rufus, Lactarius thejogalus, Leccinum scabrum, Paxillus involutus, Russula foetens, Russula rosea, Russula vesca, Suillus grevillei, Tylopilus felleus, Xerocomus badius, Xerocomus subtomentosus, pure cultures of A. muscaria, P. involutus, X. badius, X. subtomentosus, Suillus bovinus Suillus luteus and seven ectomycorrhizal morphotypes of Norway spruce were examined. Our results revealed species-specific composition of fatty acids of fungal sporocarps and pure culture mycelia. Ectomycorrhizal morphotypes distinguished and identified by morphological and molecular methods (PCR-RLFP and sequencing) created specific fatty acid profiles. The dominating fatty acids in pure cultures and sporocarps were 18:2ω6,9, 18:1ω9 and 16:0, whereas ectomycorrhizas also contained plant and bacterial specific fatty acids. Especially, fatty acids specific to Gram-positive bacteria 15:0 anteiso and 17:0 anteiso were present in relatively high amounts and suggested that these bacteria are dominating in the examined Norway spruce mycorrhizosphere. In conclusion, our results show that fatty acid based methods can be useful in studies of ectomycorrhizal fungi, both as a quick method for differentiation of fungal species and also in studies of mycorrhiza-associated microorganisms in the field. 相似文献
973.
Fernanda Bortolini Miguel Dall’Agnol Maria Teresa Schifino-Wittmann 《Genetic Resources and Crop Evolution》2006,53(5):1081-1087
White clover is one of the most important forage legume species worldwide, playing an important role in Southern Brazil temperate
cultivated pastures. This work was aimed to characterize the genetic variability of the USDA white clover core collection
formed by 78 accessions representing 50 countries, together with two very well known cultivars (Huia and Ladino Regal), using
RAPD (Random Amplified Polymorphic DNA) markers to produce genetic fingerprints. There were used DNA bulks formed by the extraction
and mixture of 20 random individuals from each accession. Twenty four primers were used, which revealed from 3 to 29 bands,
forming a total of 371 polymorphic bans and only one monomorphic, ranging from 50 to 3098 bp. The results showed a genetic
similarity among the accessions, ranging from 0.18 to 0.58 (Jaccard’s index), with an average of 0.24, allowing the identification
of each individual accession using just three primers. The results also showed a large genetic variability within the white
clover core collection, probably due to its reproduction mode and ploidy level, which could be used in plant breeding program. 相似文献
974.
Inter Simple Sequence Repeat (ISSR) Analysis of Diploid Coffee Species and Cultivated Coffea arabica L. from Tanzania 总被引:1,自引:0,他引:1
Inter simple sequence repeat (ISSR) markers were used to evaluate levels of genetic similarity among Coffea arabica L. accessions from Tanzania and to estimate levels of genetic similarities in C. arabica and diploid coffee species. The six ISSR primers used generated a total of 82 fragments and the dissimilarity values ranged
from 0.21 to 1. Mean dissimilarity values between provenances (0.56–0.85) were higher than within provenances (0.37–0.68).
Cluster analysis based on Nei’s genetic distances showed C. arabica provenances grouping based on geographical origin. Two major clusters were formed that constituted of provenances from Kilimanjaro
and Arusha in one sub-cluster; Tanga and Morogoro in the other; the second cluster had Mbeya provenances and diploid species,
respectively. The implication is that Mbeya provenances are different from the rest of Tanzanian C. arabica. A principal coordinate analysis (PCA), whose first three coordinates explained 43% of the variation, showed similar groupings
as in the cluster analysis. A separate cluster analysis of diploid species showed a distinct separation of the three species
used. ISSR data gave results similar to previous findings from random amplified polymorphic DNA(RAPD) analysis. The results
also confirm the limited diversity present in cultivated C. arabica in Tanzania 相似文献
975.
鄱阳湖生态经济区畜禽养殖土壤环境承载力及污染风险研究 总被引:3,自引:0,他引:3
[目的]测算和评价鄱阳湖生态经济区畜禽养殖过程中的土壤环境承载力及其污染风险,为合理规划鄱阳湖生态经济区畜禽养殖空间布局、实现可持续养殖提供参考。[方法]利用2000—2012年鄱阳湖生态经济区24个县(市)的统计数据进行土壤环境承载力及畜禽污染物排放量的测算和评估。[结果]2000—2012年鄱阳湖生态经济区畜禽粪便耕地负荷警报值在0.7左右,总氮耕地负载警报值为0.5~0.6,总磷耕地负载警报值超过了1,畜禽养殖对环境造成了污染威胁。畜禽养殖污染风险较高的地区为高安市、东乡县、余江县、德安县以及南昌县。[结论]污染风险较高的地区应强制实行畜禽养殖总量控制和污染物消减措施,而湖口县、都昌县和彭泽县畜禽污染风险较低,可以适当扩大养殖规模。 相似文献
976.
淮山品种"桂淮2号"的选育及栽培研究 总被引:6,自引:0,他引:6
淮山为薯蓣科薯蓣属多年生蔓性块茎类植物,是药食兼用的高效经济作物。通过优良单株筛选,结合群体观察以及植物学、生理学、产量和营养品质等相关研究,从广西地方种质资源中选育出淮山品种“桂淮2号”,并介绍其特征特性和田间肥水管理及病虫害防治等栽培技术要点。该品种具有淀粉含量高、矿质营养丰富等特点,可作为药材加工和菜用、粮用、饲用等。 相似文献
977.
Management policies to save threatened species are not always successful, often due to the lack of a scientific basis and evaluation of the species response. We describe the ecological studies and the conservation actions taken between 1985 and 1992 on Cousin Island (29 ha, Seychelles) to safeguard the future of the highly threatened Seychelles warbler (Acrocephalus sechellensis), which until 1988 only occurred on this island. A detailed field study was designed to (1) identify the key processes influencing warbler demography, (2) identify appropriate management techniques to increase the warbler population, and (3), assess the influence of the resulting habitat management. Since 1980 the island has been saturated with c. 115 territories and c. 320 birds. The warbler is purely insectivorous. Morinda (Morinda citrifolia), the most insect rich tree, is preferred for foraging. The higher the insect abundance (and Morinda cover) in territories the higher the reproductive success and survival of warblers. Insect numbers were highest in the central part of Cousin and decreased towards the coast. Coastal territories protected by a salt tolerant hedge of Scaevola (Scaevola taccada) had more insects and higher reproductive success than unprotected territories. Between 1990 and 1992 Morinda trees were planted on the island and Scaevola along the coast. Although these habitat restoration measures have not resulted in higher numbers of adult warblers and territories due to habitat saturation, they have been successful in terms of improving the quality of existing breeding territories and with that the reproductive success of breeding birds (including the number of territories producing recruits), and the exchange of individuals (genetic material) between territories. We provide evidence that the high reproductive potential of this species is likely to improve the resilience of the species to catastrophic events. 相似文献
978.
黎中宝 《中国生态农业学报》2006,14(1):16-20
阐述了近年来鲍分子遗传学方面的研究进展,总结了核型分析、等位酶、微卫星和小卫星、随机扩增多态性DNA、限制性片断长度多态性、线粒体DNA、表达序列标签研究和基因序列等技术在鲍种群遗传多样性、遗传分化、遗传结构及种质鉴定等方面的应用。并指出今后应加强鲍蛋白质组学、功能基因组学、遗传连锁图谱、数量性状基因座和标记辅助选择等方面研究。 相似文献
979.
980.