日粮精粗比对瘤胃内环境及微生物区系影响的研究

瘤胃作为反刍动物体内的饲料加工厂,其内环境稳定和微生物区系的平衡影响着动物对饲料的利用,日粮精粗比的改变影响瘤胃内环境稳态以及微生物区系组成,从而影响反刍动物的生产效率。本文综述了日粮精粗比影响瘤胃pH、NH3-N浓度、VFA浓度和瘤胃微生物区系组成的研究进展,为在生产实践中提高反刍动物生产效率提供理论依据。     

肥料运筹方式对不同用途大麦产量和品质的影响

为研究肥料运筹方式对不同用途大麦品种产量和品质的影响,以啤用大麦品种扬农啤7号和饲食兼用大麦品种扬饲麦3号为材料,分析4种肥料运筹方式对大麦籽粒产量和蛋白质含量及麦芽品质的影响。结果表明,在中等偏上肥力的土壤上,扬农啤7号优质高产施肥方式为基施尿素150 kg·hm^-2+复合肥375 kg·hm^-2,苗肥施尿素75 kg·hm^-2,拔节肥施尿素75 kg·hm^-2+复合肥225 kg·hm^-2,其产量水平在7 500 kg·hm^-2左右;主要啤酒麦芽品质接近国标二级,可适当减少氮肥用量及提前施用拔节肥改善扬农啤7号籽粒的麦芽品质。无论哪种肥料运筹方式,扬饲麦3号的麦芽品质指标均达不到国家二级麦芽标准,该品种不适宜作为啤酒大麦原料种植,宜作优质高产饲食大麦种植,其较佳肥料运筹方式为基施尿素75 kg·hm^-2+复合肥750 kg·hm^-2,拔节肥施尿素112.5 kg·hm^-2+复合肥375 kg·hm^-2,其产量水平在8 000 kg·hm^-2左右,籽粒蛋白质含量在14.0%左右。     

珍贵阔叶树种多树种混交造林技术

针对中国南方地区人工林树种林种单一、稳定性较差、物种多样性低,以至于生态服务功能低下,优质森林资源储备不足的问题,从改善林种结构,提高林分单位面积产量及生态效益出发,开展了南方主要珍贵阔叶树种多树种混交造林技术研究。结果表明,采用多树种混交模式,树种生长显著加快,单位面积总蓄积量增加,且在促进林木胸径生长方面效果显著,有利于大径材的培育;此外,多树种混交能够降低凋落物层C/N,养分分解转化快,生态稳定性更强。因此,珍贵树种多树种混交栽培模式具有可行性。     

石油胁迫下混播对玉米幼苗叶绿素含量的影响

[目的]为混播种植方式在提高玉米幼苗光合能力以及油污土壤生物修复中的应用提供科学依据。[方法]在人工模拟石油污染土壤条件下,采用盆栽方法探讨石油胁迫下混播对玉米幼苗叶绿素含量的影响。[结果]石油污染对玉米幼苗叶绿素的形成有抑制作用;无论在常规还是在石油污染的备件下,不同品种玉米与大豆混播值均高于相应品种玉米单播值。[结论]混播对土壤石油污染表现出一定的耐受性,在玉米增产和油污土壤植物修复方面能够起一定的指导作用。     

高致病性猪蓝耳病毒及猪圆环病毒2型混合感染的诊断

[目的]对湖北省某猪场发生的以体温升高、部分病猪死亡等为主要特征的疾病进行诊断。[方法]采集发病猪的脾脏、淋巴结等样品,应用PCR方法进行实验室检测。[结果]经实验室检验,结合临床症状,诊断为HP．PRRSV及PCV2混合感染。高致病性猪蓝耳病毒阳性率为40％,猪圆环病毒2型阳性率为90％。[结论]通过采取综合防控措施,病情得到有效控制,减少了猪场的经济损失。     

0．5％毒死蜱药肥混剂对韭蛆的田间防治效果

[目的]评价0．5％毒死蜱药肥混剂对韭菜韭蛆的防治效果。[方法]于2012～2013年,在山东省烟台市福山区进行了0．5％毒死蜱药肥混剂防治韭菜韭蛆田间药效试验。[结果]0．5％毒死蜱药肥混剂在1875g／hm2施用量下,2012和2013年药后21d的防效分别达77．15％和80．00％,21d虫口防效分别达99．55％和97．30％,增产效果分别为59．06％和30．09％,显著高于对照药剂5％毒死蜱颗粒剂。[结论]应用0．5％毒死蜱药肥混剂防治韭蛆,推荐使用剂量为l125～1875g／hm2。     

蔬菜茎叶混合青贮技术及其应用研究进展

蔬菜茎叶青绿多汁,适口性好,含有丰富的粗蛋白、粗脂肪和粗纤维,是牲畜的优质饲料.然而,除了小部分作为青绿饲料直接饲喂动物外,大部分蔬菜茎叶下脚料被遗弃或抛弃,这不仅造成资源浪费,而且造成环境污染.通过青贮等方式将蔬菜茎叶加工成动物饲料,既可以提高其利用价值,又可以取得良好的社会效益.对蔬菜茎叶混合青贮技术、影响青贮效果的因素及其在养殖业中的应用进行了综述.     

除草剂混合标准溶液中标准物质稳定性研究

选取德国DR公司生产的28种除草剂标准物质,将其配制成混合标准溶液后转移至20 mL带聚四氟乙烯衬盖的棕色螺口样品瓶中,于-18℃避光贮存,采用液相色谱串联质谱法测试各组分量值随贮存时间的变化。结果表明:在90天内,标准物质各组分量值的标准偏差在12~73μg/L范围内,最大相对标准偏差为3.04%(n=5),测量结果的相对不确定度小于5.60%。在置信度为95%时,标准物质各组分量值在(1 000±113)μg/L范围内,测量的偏差不显著。在不超过标准物质的有效期内,稀释混合分装后的标准物质可保存90天。     

The biodiversity of dictyostelids in mountain forests: A case study in the French Alps

Forest management can seriously modify the biodiversity of forest dwelling species, but the consequences are poorly known for certain taxa, particularly soil fauna, for which few studies have been published. We compared the biodiversity of dictyostelids cellular slime moulds in a managed and an unmanaged forest in the French Alps and analysed the influence of environmental factors on species richness and abundance of dictyostelids. To our knowledge, this study is the first one undertaken in the European Alps. We must better understand the influence of various environmental factors on the biodiversity of these organisms if we want to accurately define their functional role in the soil. In our study, dictyostelids showed lower levels of diversity compared to previously published results. The mean species richness of dictyostelids was marginally higher in unmanaged than in managed forests and biodiversity indices were significantly correlated with elevation and pH. This suggests that environmental factors have a predominant effect on the biodiversity of dictyostelids and that the effect of forest management is secondary.     

Carbon dioxide emissions of soils under pure and mixed stands of beech and spruce, affected by decomposing foliage litter mixtures

Soil respiration is the largest terrestrial source of CO₂ to the atmosphere. In forests, roughly half of the soil respiration is autotrophic (mainly root respiration) while the remainder is heterotrophic, originating from decomposition of soil organic matter. Decomposition is an important process for cycling of nutrients in forest ecosystems. Hence, tree species induced changes may have a great impact on atmospheric CO₂ concentrations. Since studies on the combined effects of beech-spruce mixtures are very rare, we firstly measured CO₂ emission rates in three adjacent stands of pure spruce (Picea abies), mixed spruce-beech and pure beech (Fagus sylvatica) on three base-rich sites (Flysch) and three base-poor sites (Molasse; yielding a total of 18 stands) during two summer periods using the closed chamber method. CO₂ emissions were higher on the well-aerated sandy soils on Molasse than on the clayey soils on Flysch, characterized by frequent water logging. Mean CO₂ effluxes increased from spruce (41) over the mixed (55) to the beech (59) stands on Molasse, while tree species effects were lower on Flysch (30-35, mixed > beech = spruce; all data in mg CO₂-C m⁻² h⁻¹). Secondly, we studied decomposition after fourfold litter manipulations at the 6 mixed species stands: the O_i - and O_e horizons were removed and replaced by additions of beech -, spruce - and mixed litter of the adjacent pure stands of known chemical quality and one zero addition (blank) in open rings (20 cm inner diameter), which were covered with meshes to exclude fresh litter fall. Mass loss within two years amounted to 61-68% on Flysch and 36-44% on Molasse, indicating non-additive mixed species effects (mixed litter showed highest mass loss). However, base cation release showed a linear response, increasing from the spruce - over the mixed - to the beech litter. The differences in N release (immobilization) resulted in a characteristic converging trend in C/N ratios for all litter compositions on both bedrocks during decomposition. In the summers 2006 and 2007 we measured CO₂ efflux from these manipulated areas (a closed chamber fits exactly over such a ring) as field indicator of the microbial activity. Net fluxes (subtracting the so-called blank values) are considered an indicator of litter induced changes only and increased on both bedrocks from the spruce - over the mixed - to the beech litter. According to these measurements, decomposing litter contributed between 22-32% (Flysch) and 11-28% (Molasse) to total soil respiration, strengthening its role within the global carbon cycle.