Comparison of sampling sites and detection methods for Haemophilus parasuis

Objective To improve the isolation rate and identification procedures for Haemophilus parasuis from pig tissues. Design Thirteen sampling sites and up to three methods were used to confirm the presence of H. parasuis in pigs after experimental challenge. Procedure Colostrum‐deprived, naturally farrowed pigs were challenged intratracheally with H parasuis serovar 12 or 4. Samples taken during necropsy were either inoculated onto culture plates, processed directly for PCR or enriched prior to being processed for PCR. The recovery of H parasuis from different sampling sites and using different sampling methods was compared for each serovar. Results H parasuis was recovered from several sample sites for all serovar 12 challenged pigs, while the trachea was the only positive site for all pigs following serovar 4 challenge. The method of solid medium culture of swabs, and confirmation of the identity of cultured bacteria by PCR, resulted in 38% and 14% more positive results on a site basis for serovars 12 and 4, retrospectively, than direct PCR on the swabs. This difference was significant in the serovar 12 challenge. Conclusion Conventional culture proved to be more effective in detecting H parasuis than direct PCR or PCR on enrichment broths. For subacute (serovar 4) infections, the most successful sites for culture or direct PCR were pleural fluid, peritoneal fibrin and fluid, lung and pericardial fluid. For acute (serovar 12) infections, the best sites were lung, heart blood, affected joints and brain. The methodologies and key sampling sites identified in this study will enable improved isolation of H parasuis and aid the diagnosis of Glässer's disease.     

Antioxidant,anti-amylase and anti-glycation potential of brans of some Sri Lankan traditional and improved rice (Oryza sativa L.) varieties

Brans of 23 traditional and 12 improved (both red and white) rice varieties in Sri Lanka were screened for anti-amylase and anti-glycation activities in vitro. Varieties which showed the highest inhibitory activities at screening were further investigated for anti-glucosidase and glycation reversing as anti-diabetic properties. The same varieties were studied for selected antioxidant properties. Significantly high anti-amylase and anti-glycation activities were observed for bran extracts of red varieties compared to white varieties at screening. Traditional red rice varieties, Masuran, Sudu Heeneti, Dik Wee and Goda Heeneti, exhibited significant and dose dependent anti-amylase, anti-glycation and glycation reversing activities. These varieties also showed marked antioxidant properties. It is concluded that brans of Sri Lankan traditional red rice varieties Masuran, Sudu Heeneti, Dik Wee and Goda Heeneti may be potential food supplements for diabetes.     

绿藤长茄新品种“慈茄2号”选育初探

在慈溪市白沙路街道现代农业示范园区,以韩家路黑藤茄为母本,以天元长茄为父本,杂交而产生变异杂合体,再通过系统选育方法选育成了绿藤长茄新品种——慈茄2号。其表现中熟,定植到采收40 d左右,自开花到商品嫩果采收18 d左右,全生育期130 d;茎、叶、果皮均为绿色,果实长条形,呈弯曲状;长势旺盛,耐热、耐寒、耐涝,连续结果能力强,产量高,品质好。     

外源褪黑素对‘红地球’葡萄采后贮藏品质的影响

研究不同浓度的外源褪黑素对‘红地球’葡萄采后品质的影响,为葡萄保鲜技术提供一定的数据支撑和理论依据。以‘红地球’葡萄品种为试验材料,采用不同浓度0μmol/L（ck）、50μmol/L（T1）、100μmol/L（T2）、200μmol/L（T3）、400μmol/L（T4）的外源褪黑素浸泡‘红地球’葡萄果实0.5 h,常温贮藏,测定果实的失重率、硬度、可溶性固形物、可溶性糖、可滴定酸、Vc、PPO、POD、MDA等指标的变化。结果表明,贮藏到第16 d时,ck组霉变严重,失重率达到35.4%,而外源褪黑素处理组均只有轻微霉点,失重率为23.8%~33.2%,且可溶性糖、MDA、PPO、POD含量均显著低于ck组;果实硬度、可溶性固形物、可滴定酸、Vc等含量均显著高于ck组。说明外源褪黑素延缓‘红地球’葡萄在贮藏过程中因氧化而引起的果实霉变,维持葡萄果实储藏期间的品质,保持果实良好的口感,其中,以浓度为100 μmol/L的外源褪黑素贮藏保鲜效果最佳。     

琯溪蜜柚砂囊粒化的无损检测

摘 要：柑橘砂囊粒化是柑果成熟期与采后贮藏期的常见生理性病害,严重影响果实品质。本文采用1.5 T 高场磁共振成像仪对采后贮藏30 d的琯溪蜜柚[Citrus grandis (L.) Osbeck]果实进行磁共振扫描检查,并以同层断面解剖图为对照,评估磁共振成像（MRI）应用于柑橘砂囊粒化症无损检测的有效性。结果表明, MRI检测中,T2WI能够显著区分果肉组织与海绵层组织的信号差异,其信号强度可代表果实组织相对含水量;T2WI信号在砂囊粒化部位强度显著降低,病变组织的失水特征清晰,与解剖结果一致。T2WI对砂囊粒化的确诊率100%,显著优于T1WI成像的识别率41.7%（χ2 = 5.76,P = 0.016）;T2WI和T1WI均能显示种籽照影,但T2WI对果肉组织影像的分辨率优于T1WI,因此MRI的T2WI分析可作为一种无损检测方法,对柚商品果实上市前砂囊粒化症发生程度以及种籽有无进行评估。     

China's alfalfa market and imports: Development,trends, and potential impacts of the U.S.–China trade dispute and retaliations

This study examines the development and trends of China's alfalfa market and imports, identifies key factors for the rapid increase in China's alfalfa imports, and discusses potential impacts of the U.S.–China trade dispute and retaliations on the alfalfa markets and trade in both nations. China's rapid transition toward larger-scale commercial dairy production, with enhanced feed and cost management as well as quality and safety control, and its limited resources for high-quality alfalfa production are key factors for the dramatic increase in its alfalfa imports, from 19 601 metric tons in 2008 to 1.38 million metric tons (mmt) in 2018. While the United States dominated China's alfalfa imports with an average share of 97.01% from 2007 to 2017, the share dropped to 83.76% in 2018 and 63.28% in January 2019 due to the trade dispute and retaliations started in 2018. China will likely remain a large importer of alfalfa because of both its growing demand and the comparative advantages of imported alfalfa in quality and price, but the imports from the United States will be highly affected by the ongoing trade dispute and negotiations. China is also expected to make more efforts to reduce its dependence on U.S. alfalfa through increased investment in domestic alfalfa production and identification of alternative sources of alfalfa and other hay imports.     

Adoption and Impact of Modern Rice Varieties on Poverty in Eastern India

The factors affecting the adoption of modern varieties(MVs) of rice and impact on poverty in Odisha, India were discussed. A total of 363 households from Cuttack and Sambalpur districts of Odisha via multistage sampling technique participated in the survey. The Cragg's Double hurdle model was used to model the determinants of adoption and intensity of adoption of MVs of rice, and the propensity score matching was used to analyze the impact of adoption on poverty. The results showed that age, education, risk aversion, land size, yield, perception of MVs as high yielding, resistant to diseases and availability of MVs positively influenced the decision to adopt. However, variables such as household size, experience of a farmer, off-farm job participation, amount of credit received, cost of seeds, insecticides and fertilizers negatively influenced the adoption of MVs. Intensity of adoption of MVs was negatively influenced by experience of a farmer, cost of fertilizer and marketability of MVs, and positively affected by household size, risk aversion, land size, cost of insecticides, perception of MVs as high yielding and availability of MV seeds. Poverty incidence, gap and severity were high among non-adopters to adopters of MVs. After matching adopters and non-adopters of MV groups using four different algorithms of nearest neighbour matching, stratification matching, radius matching and kernel matching, the impact of MV adoption resulted in higher per capita monthly household expenditure by about US$ 52.82 to US$ 63.17.     

智慧农业背景下大数据与农民增收之间的关系

自信息化时代以来,大数据时代是继物联网、云计算的又一次技术变革,这次变革加速了传统农业向数字农业、信息农业的转变。智慧农业的发展,创造了多维的与农业有关的数据。阐述了在农产品种植前的规划、种植时的管理和收获之后的营销,在已有的数据中挖掘可用的信息,农民可以准确掌握市场供需情况,合理安排种植,做到精准农业,帮助农民提升收入。