苜蓿雄性不育系组织培养技术的研究

以苜蓿雄性不育系Ms-4幼嫩茎段、叶片、叶柄为外植体,诱导苜蓿雄性不育系再生植株.结果表明:叶柄是诱导愈伤组织的良好外植体,体细胞胚诱导率为90%,再生率49%.苜蓿雄性不育系插条生根的最佳处理为接种前用水进行预处理0.5h,外植体为完整插条,培养基为A1、A2,其生根率为97%,生根系数为5.3.     

‘新高’梨雄性不育与IAA和ABA含量变化的关系

 以雄性不育的‘新高’梨和可育的‘丰水’梨为试材, 研究花粉发育过程中IAA和ABA含量及其与IAA代谢相关酶和微量元素的变化。结果表明: 丰水花蕾中IAA含量从四分体后极显著地高于新高, 在新高花粉败育期(单核花粉早期到单核花粉晚期) 是新高的3.70～4.47倍, 新高IAA含量的亏缺可能是导致败育的原因之一; 新高在花粉发育前期IAA氧化酶活性显著高于丰水, 而两个品种POD活性变化一致, 差异不显著, 说明POD不是导致新高IAA含量的亏缺的关键酶; 新高Zn含量低于丰水, 而Mn含量从单核花粉期以后却高于丰水; 新高ABA含量从四分体到二核花粉期显著高于丰水, 而丰水在成熟的花药中积累了大量的ABA。     

西瓜成熟花粉的超微结构

为了解西瓜成熟花粉的超微结构特点,特别是营养细胞和生殖细胞之间的关系,选取刚开花的西瓜雄花采集花粉,2.5%戊二醛固定并制成超薄切片,透射电镜观察照相。结果表明,西瓜成熟花粉粒中营养细胞含丰富脂类储藏物质和内质网,内质网堆叠,周围和间隙中充满脂类物质;含丰富高尔基体和大量小泡;质体很少。营养核通常深度开裂,产生多数裂片,裂片伸向生殖细胞。生殖细胞流线型,细胞壁电子透明,细胞核伸长,细胞质含高尔基体、内质网,不含质体。生殖细胞表面产生波状突起,波峰背离营养核。生殖细胞一端伸入营养核裂片间隙,2者构成雄性生殖单位。     

种公鹿茸重性状与年龄相关的统计分析

本文探讨了我国人工选育的双阳梅花鹿和西丰梅花鹿品种、长白山梅花鹿品系、天山马鹿清原品系和乌兰坝东北马鹿等 5个品种品系种公鹿的茸重性状与鹿年龄的关系 ,结果表明 ,在一定年龄段 ,茸重性状与年龄之间呈强正相关并建立了相应的线性回归方程 ,为预测并比较茸鹿种公鹿各年龄时的茸重水平和制定茸重标准及早选种等提供了科学依据     

玉米温光敏雄性不育系9417叶片叶绿体蛋白质差异分析

对玉米温光敏感雄性不育-可育 “两用系”9417的不育型和可育型叶片中的叶绿体蛋白质组进行研究，试图找出不育型与可育型的差异蛋白,确定导致不育的蛋白。利用SDS PAGE凝胶电泳，对玉米雄性不育 可育“两用系”不育型与可育型8叶期功能叶叶绿体蛋白质组中的差异蛋白进行分析。结果表明，不育型与可育型存在3条蛋白差异条带，其中No.1条带为不育型中新出现的蛋白质条带，而其余两条条带只在表达丰度上有差别。利用MALDI TOF MS方法，运用MASCOT软件于NCBI数据库中进行数据查询，结果显示No.1条带中含有3种蛋白质，而这3种差异蛋白的出现可能与玉米不育性有关。     

Convenient Estimation of Unfertilized Grains in Rice

Abstract

Failure of fertilization in rice is a critical yield-determining factor in plants subjected to temperature or water stress at the early-reproductive stage and in high-yield cultivars bearing heavy spikelets. Although it is important to identify quickly the unfertilized spikelets for research and selection of stress-resistant or high-ripening cultivars from bulksamples, the identification takes time because unfertilized spikelets are usually determined by visual and manual procedures. Our objective was to develop a convenient method to identify unfertilized spikelets in rice. Takanari spikelets at maturity grown in the paddy field were separated into floating and sinking spikelets by different specific gravity solutions of ethanol/water mixture. The unfertilized spikelets were identified by checking the grains inside the spikelets by light penetration and examining the spikelets manually. The percentage of floating spikelets decreased with the increase in ethanol concentration, and that of floating spikelets approximately coincided with the percentage of unfertilized spikelets when the specific gravity was below 0.90×10>³ kg m-³, corresponding to over 70% -ethanol. In a practical range of temperature the specific gravity scarcely changed. In an 80%-ethanol solution, the percentages of floating spikelets in Takanari grown under different nitrogen applications and in rice cultivars having different spikelet size approximately coincided with percentages of unfertilized spikelets, though the percentages of floating spikelets was 5 to 7% higher than the unfertilized spikelets. The use of 70%-ethanol solution increased the difference in some rice cultivars. We concluded that the gravitation method would be convenient for identification of unfertilized spikelets in bulk samples of rice.     

银杏雄花性状的研究

连续2a测定分析了扬州市内2株树势健壮、开花正常，花量一致的成年银杏雄株的化花序形状、花离颜色，花序大小，花柄长短，单花重，花囊形状与大小、花粉数量与大小及其淀粉含量等性状指标，结果表明，银杏雄株树龄越大，1年生枝的生长量相对较小，雄花每花囊的花粉和雄花每花序的花粉数亦相对较小，其花粉的淀粉含亦相对较低，不同雄株同雄花的单花重，花序的长、宽和长／宽；花柄的长和宽，每花序的花囊数；花粉粒直径及花粉淀粉含量具促进或极显著差异，雄花的花柄长（x1）和花序长（y1），花柄宽（x2）与花序宽（y2）及花柄长／宽（x3）与花序长／宽（y3），具有显著或极显著相关，其相关回归方程分别为^/y1＝1．7909＋0．3440x1（r1＝0．2440^**），^y2＝0．5211＋0．8378x2（r2＝0．2530^**）,^y3＝2．7455＋0．08743x3（r＝0．2237^*）。     

THE EFFECT OF LOW TEMPERATURE ADVERSITY ON MALE FLOWERS AND POLLEN IN LARIX OLGENSIS

INTRODUCTIONLarlxolgcnsisisoneofthemainlyusedspeciesintl1ecastforestareaofnortheastChina.Itgrowsthstandhashighcomprehen-six'cusedvalue.So,itisdeeplylovedbypeo-ple,tl1cafforestionarcaisenlargedgraduallyyearbyycar.needsofitsseedsbecomegrcaterandgrcater.Forsccd-bearingcyclcanddal11-agcofIatclYostinproducingseedpcriod,tl1coutputisvel3'low,andseedsforaflbrcstingarcoIlcnil1sufficient.So'itisurgenttostlldycffcctoflot"tcmperatureonfemaleandmalel1otversofIarixolgcnsissotl1atmethodsofillcrcasingf…     

银杏雄株开花生物学特性的研究

对从泰安和郯城共５０５０株银杏雄株中筛选出的２６个优良单株的开花生物学特性进行了研究。这些单株具有花期长、花粉量大及花粉生活力强等特点。相关分析和方差分析结果表明,随海拔高度的增加,花期逐渐延长,但小孢子叶球数／短枝下降。每米长枝上的短枝数最高达４５个。短枝长与小孢子叶球数／短枝呈极呈著的正相关。３年生枝段短枝开花率最高,达９６％。每个短枝上小孢子叶球数最高达６４５个,每个小孢子叶球最多含花药５８个,每个花药含有花粉粒最高达２３×１０４个,平均每个小孢子叶球含花粉粒达６５×１０４个。１４＃单株出粉率达６８３％。一株４５年生雄株可产花粉１６５ｇ。花粉生活力平均为９２％。授粉后座果率９０％,有胚率９１％。