连杆外延型曲柄摇杆机构的综合模型设计

为了改进机械锹，本文对它的核心机构—一种连杆外延而形成工作臂的曲柄摇杆机构，进行了结构及运动方面的综合分析。从而建立了该类机构的数学分析模型，并编写了相应的计算程序。由此可拓宽该机构的应用范围和提高其使用效果。     

Double haploids,markers and QTL analysis in vegetable brassicas

Double haploid (DH) plants of Brassica spp. can be produced via anther culture or culture of microspores. This paper reviews the uses of double haploids in crop improvement research in vegetable brassicas (B. oleracea). Applications of DH lines are described for breeding; construction of linkage maps; genetic analysis of quantitative traits and capturing genetic variation. The advantages and disadvantages of DH lines are discussed     

Linkage mapping and genome analysis in a Saccharum interspecific cross using AFLP, SRAP and TRAP markers

Framework genetic linkage maps of two progenitor species of cultivated sugarcane, Saccharum officinarum ‘La Striped’ (2n = 80) and S. spontaneum ‘SES 147B’ (2n = 64) were constructed using amplified fragment length polymorphism (AFLP), sequence related amplified polymorphism (SRAP), and target region amplification polymorphism (TRAP) markers. The mapping population was comprised of 100 F₁ progeny derived from the interspecific cross. A total of 344 polymorphic markers were generated from the female (S. officinarum) parent, out of which 247 (72%) were single-dose (segregating in a 1:1 ratio) and 33 (9%) were double-dose (segregating in a 3.3:1 ratio) markers. Sixty-four (19%) markers deviated from Mendelian segregation ratios. In the S. spontaneum genome, out of a total of 306 markers, 221 (72%) were single-dose, 43 (14%) were double-dose, and 42 markers (14%) deviated from Mendelian segregation ratios. Linkage maps with Kosambi map distances were constructed using a LOD score ≥5.0 and a recombination threshold of 0.45. In Saccharum officinarum, 146 markers were linked to form 49 linkage groups (LG) spanning 1732 cM whereas, in S. spontaneum, 121 markers were linked to form 45 LG spanning 1491 cM. The estimated genome size of S. officinarum ‘La Striped’ was 2448 cM whereas that of S. spontaneum ‘SES 147B’ was 3232 cM. Based on the two maps, genome coverage was 69% in S. officinarum and 46% in S. spontaneum. The S. officinarum parent ‘La Striped’ behaved like an auto-allopolyploid whereas S. spontaneum ‘SES 147B’ behaved like a true autopolyploid. Although a large disparity exists between the two genomes, the existence of simple duplex markers, which are heterozygous in both parents and segregate 3:1 in the progeny, indicates that pairing and recombination can occur between the two genomes. The study also revealed that, compared with AFLP, the SRAP and TRAP markers appear less effective at generating a large number of genome-wide markers for linkage mapping in sugarcane. However, SRAP and TRAP markers can be useful for QTL mapping because of their ability to target gene-rich regions of the genome, which is a focus of our future research.     

乡村生态旅游与运动休闲产业的联动研究——以浙江龙游县为例

分析了浙江龙游县乡村生态旅游与运动休闲产业联动的可行性,提出了联动的模式和具体的策略。     

家蚕突变基因分析和基因资源库的建立及应用研究

通过家蚕品种资源的基因调查，对８０多个突变基因进行了基因分析；确立了家蚕２８个连锁群的标志基因或代表基因；建立了较完整的家蚕基因资源库；修改了家蚕第１８连锁群图，并获得国际学术界公认和采纳。利用形态性状标记抗浓核病基因，研究出一种利用形态标记进行抗浓核病品种选育的新方法；利用突变基因，选育出几个有特殊用途的育种素材及基础材料。     

连杆外延型曲柄摇杆机构的一种图谱分类法初探

本文在对连杆外延而形成工作臂的曲柄摇杆机构进行综合分析的基础上，着重对该类机构工作臂上工作点的运动特性进行了分析，揭示了机构工作点的运动变化与机构结构之间的内在联系，从而提出了该类机构的一种具有较强直观性、实用性、启发性和可比性的工作点运行轨迹图图谱分类法。     

应用二花脸与大约克猪建立参考家系进行连锁分析的研究

应用１头德国大约克公猪及３头二花脸母猪通过回交法（父女交配）建立了参考家系，采集了１５４头个体的血样，对１２号染色体 微卫星位点（ＳＷ９５７、ＳＷ１５５３、ＳＷ１３５０、ＳＷ８７４）检测了每个个体的基因型，应用ＣＲＩＭＡＰ程序进行了连锁分析得出了３个位点在猪１２号梁色体上的最佳排序为：ＳＷ１５５３－ＳＷ１３５０－ＳＷ８７４。其中在ＵＳＤＡ－ＭＡＲＣ家系中由于未检测到重组而不能排序的两个位点（ＳＷ１     

城乡建设用地增减挂钩政策中农民意愿及区域差异研究——以江苏省为例

采用实地调查方法对江苏省典型地区城乡建设用地增减挂钩(LUTRG)政策中农民意愿进行研究,分析增减挂钩中农民的参与意愿、拆迁意愿、安置意愿及满意度,并探讨农民意愿的区域差异性。研究结果表明,多数农民有参与增减挂钩的意愿,但农民意愿分化严重;农民希望提高拆迁补偿标准与补偿过程透明化;农民倾向于集中安置,并十分关注安置小区配套设施完善情况;生活成本与就业是农民被安置于城镇后关注的重点;不同地区农民参与意愿、关注点及满意度存在差异。     

两个玉米回交一代群体中opaque2基因单侧连锁累赘的SSR分析

为了在回交一代既能较好地消除靶基因的遗传连锁累赘，又能在进行背景选择的基础上选择到轮回亲本遗传背景回复率较高的单株，本研究构建了单株数分别为1 416和1 627的SCBC₁F₁和HCBC₁F₁两个不同遗传背景的回交群体。结合玉米叶片DNA大量、快速提取法，使用5个与opaque2（o2）基因连锁并已定位的SSR标记，在SCBC₁F₁与HCBC₁F₁群体中，对o2基因单侧的连锁累赘进行SSR分析，并与Ribaut（2002）的计算机模拟结果进行比较，结果表明，本研究获得的结果优于相应的计算机模拟结果。并对分子标记辅助选择体系在玉米回交育种中应用的若干理论问题进行了讨论，认为在实际应用MAS程序中，不仅要重点考虑最终决选的单株数（N_i），而且还应该考虑到实际有足够后代的中选单株数（N_j）。另外，结合预期要消除连锁累赘的图距，制备足够大的BC₁F₁群体是十分必要的。     

螺杆膨化挤压机套筒离合驱动机构设计研究

对螺杆膨化挤压机套筒离合驱动机构装置进行优化设计,在现有螺杆挤压机下端安装液压驱动机构以实现套简的机械开合。对设计的套筒离合驱动机构动力学模型进行理论计算,经验证该装置结构安全、操作简单、维护方便,具有很大的社会应用价值。