CO2浓度升高条件下水稻蒸腾与N吸收的关系

利用FACE（Free Air Carbon Dioxide Enrichment）平台技术,用水培试验研究了低氮（14 mg/L）和高氮（28 mg/L）水平下,大气CO2浓度升高条件下水稻蒸腾与N吸收速率的相关关系。结果表明,在CO2浓度升高条件下,水稻生物量增加了36%（低N）和29%（高N）;总吸N量也增加达7%（低N） 和5%（高N）;而总蒸腾量减少28%（低N）和10%（高N）。由于促进更多分蘖的发生,高CO2浓度使分蘖期水稻平均N吸收速率提高了31%～156%（低N）和19%～87%（高N）,在其他时期无明显影响;而高CO2浓度对水稻平均蒸腾速率的影响主要表现在抽穗到灌浆末期。在对照条件下,平均蒸腾速率和平均N吸收速率呈显著正相关;但在CO2浓度升高条件下,两者相关关系不显著。说明人们所推测的“蒸腾效应”——高CO2浓度条件下降低了的蒸腾作用并非影响水稻N吸收的关键因素。     

Cuticular uptake of xenobiotics into living plants. Part 2: influence of the xenobiotic dose on the uptake of bentazone, epoxiconazole and pyraclostrobin, applied in the presence of various surfactants, into Chenopodium album, Sinapis alba and Triticum aestivum leaves

This study has determined the uptake of three pesticides, applied as commercial or model formulations in the presence of a wide range of surfactants, into the leaves of three plant species (bentazone into Chenopodium album L. and Sinapis alba L., epoxiconazole and pyraclostrobin into Triticum aestivum L.). The results have confirmed previous findings that the initial dose (nmol mm(-2)) of xenobiotic applied to plant foliage is a strong, positive determinant of uptake. This held true for all the pesticide formulations studied, although surfactant concentration was found to have an effect. The lower surfactant concentrations studied showed an inferior relationship between the amount of xenobiotic applied and uptake. High molecular mass surfactants also produced much lower uptake than expected from the dose uptake equations in specific situations.     

Use of the Genetically Engineered Agrobacterium Strain K1026 for Biological Control of Crown Gall

Strain K84 of Agrobacterium (formerly called A. radiobacter) has been a successful biocontrol agent of crown gall disease for almost 30 years all over the world. In spite of its demonstrated efficiency, the most important risk of failure when using strain K84 is the possibility of transfer of plasmid pAgK84 to pathogenic Agrobacterium strains. pAgK84 codifies production of and immunity to agrocin 84, the main factor involved in crown gall biocontrol by strain K84. Then, a second generation of strain K84 was obtained and the genetically engineered strain was called K1026. It contains a deletion in the transfer region of pAgK84. To date, a considerable number of studies have been performed to compare both strains in its ability to control crown gall, plasmid transfer, antibiotic production, root colonization and survival in the rhizosphere. The aim of this review is to discuss all this comparative available information which advises that strain K1026 should be used as a biopesticide to safeguard biocontrol of crown gall wherever strain K84 is employed.     

超高产杂交稻剑叶的光抑制及其77K荧光光谱特性

通过对培矮64S/E32和汕优63进行高光胁迫结果表明: 与籼型杂交稻汕优63比较, 培矮64S/E32的Fv/Fm和ΦPSⅡ、PSⅡ和PSⅠ荧光强度下降的幅度以及F685/F742和F694/F742比率的变化较小。用D1蛋白合成抑制剂CAP处理时,培矮64S/E32 的Fv/Fm和qP下降程度稍高于汕优63,而用叶黄素循环抑制剂DTT处理,结果则反之。表明超高产水稻品     

水稻高亲和力磷酸盐转运蛋白功能的初步研究

以所分离到的目的片段为基础,采用3'RACE技术获得编码水稻磷酸盐转运蛋白基因的全序列,ORF区为含535氨基酸的基因,具有保守的酪蛋白激酶Ⅱ、N糖基化与蛋白激酶C作用位点的序列。通过对此蛋白编码基因功能的初步研究,认为此基因具有增加植物在缺磷胁迫下根系吸磷的能力,转基因的愈伤组织在低磷处理下具有相对于对照愈伤组织较高的吸磷速率。经对愈伤组织的PCR与PCR Southern 杂交分析,吸磷速率高的愈伤组织为遗传转化的阳性植株。     

氮肥基因型差异对玉米吸氮量的反应

田间试验研究了11个玉米杂交种在不同施氮条件下的吸氮量。结果表明,玉米吸氮量具有明显的基因型差异。在低氮(N2)处理中,子粒、秸秆、地上部吸氮量高低品种相差分别为1.060、0.134、1.125g/kg,在高氮(N4)处理中,分别为0.836、0.154、0.954g/kg。不同基因型玉米的吸氮量差异低氮条件比高氮条件更大。施氮量对玉米吸氮量有显著的影响。玉米子粒、秸秆、地上部吸氮量和相应的产量呈极显著的正相关     

栽培大豆蛋白亚基11S/7S组成及过敏蛋白缺失分析

以175份中国大豆品种资源为材料,利用SDS-PAGE电泳和Western杂交技术,分析蛋白亚基11S和7S的含量,以及Gly m Bd 28K和 Gly m Bd 30K两种过敏蛋白的缺失情况。结果表明,参试品种的11S和7S的含量呈极显著负相关（r＝－0.95）,11S/7S比值范围为0.77～4.67,并鉴定出1份自然缺失β亚基的材料。在175个品种中没有检测到缺失30     

土壤速效磷、速效钾常规测定值与速测法测定值相关性研究

对山西省晋中、忻州、吕梁、运城、晋城、长治等地市不同肥力水平 0～ 2 0cm的 1 0 0个土样 ,分别采用常规分析与速测法 (目测 )对各土样中速效磷 ,速效钾进行测定 ,以探索速测与常规分析法测定值间的相关性。通过回归分析对数据的处理、统计分析 ,表明两种方法测定值间相关性显著 ,并建立了它们之间的线性转换模式 (1 )、(2 )。 (本模式适用于石灰性土壤 )     

水稻对~(65)Zn吸收和分配的比较研究

本试验以籽粒高锌含量基因型水稻V5 6和低锌含量基因型水稻湘早籼 1 7为材料 ,运用溶液培养和同位素示踪技术 ,探讨了水稻不同时期对65Zn吸收、运转和分配 ,特别是往籽粒的运输与分配。试验结果表明 :V5 6苗期根系吸收65Zn的能力和往地上部运转65Zn的能力强 ,累积的65Zn较少 ;生殖阶段分配到剑叶的65Zn低 ,籽粒的65Zn分配率高 ,籽粒65Zn累积高。湘早籼 1 7的结果恰恰相反     

魔芋（Amorphophallus rivieri Durieu）叶面积测定方法的？…

以叶面积仪实测的魔芋（Ａｍｏｒｐｈｏｐｈａｌｌｕｓ ｒｉｖｉｅｒｉ Ｄｕｒｉｅｕ）叶面积为参照,分别获得了用叶片上各小裂片的长径（Ｌ）与短径（Ｗ）计算叶面积的校正值（Ｋ１＝０．６３）和用叶片上３个羽片的长度（Ｄ）与羽片的开张度（Ｅ）计算叶面积的校正值（Ｋ２＝０．５２）;得到了用Ｌ和Ｗ计算的单株叶面积为０．６３∑ｎｉ＝１ＬｉＷｉ（ｎ为小裂片总数）,用Ｄ和Ｅ计算的单株叶面积为０．５２∑ｉ＝１ＤｉＥｉ（