木芙蓉扦插繁殖研究

在塑料拱棚下进行木芙蓉扦插繁殖试验,研究不同类型插穗、不同扦插基质以及不同生根粉（IBA）浓度对木芙蓉扦插生根成活率的影响．试验结果表明：丰木质化枝条比嫩枝生根成活率高;不同浓度的IBA处理中以1000mg／kg效果最好,生根成活率达86．7％;3种不同扦插基质中,以山砂基质的扦插成活率最高．扦插繁殖的最佳组合是：采用半木质化枝条作插穗,经1000mg／kg浓度IBA处理,在山砂基质上扦插．     

不同生长素处理条件下杂种落叶松生根力的变异

目前广泛应用于促进插穗生根的外源生长素有吲哚丁酸(IBA)、萘乙酸(NAA)、吲哚乙酸(IAA),以及商业化的植物生根促进剂,其中IBA效果最为突出(王建华等,2006;祁德富等,2007;张卫国等,2007).     

不同浓度IBA对大果榛子不同品种黄化萌条扦插成活率影响综合评价

【目的】研究大果榛子黄化萌条扦插苗成活率和生根质量,有效提高黄化萌条的利用率。【方法】以大果榛子品种新榛1号、新榛2号和新榛3号的黄化萌条作为试验材料,研究不同浓度吲哚丁酸(IBA)对其扦插生根率、根生长量特征参数和地上部分生长特征参数的影响,采用隶属函数进行综合评价。【结果】不同浓度IBA对大果榛子不同品种黄化萌条扦插生根率、根生长量特征参数和地上部分生长特征参数的影响有所差异。IBA浓度为5 mmol/L时,新榛1号的生根范围、单株新梢数、单株新梢长度和新梢饱满芽数均最高;新榛2号的生根率、生根指数和单株根数均最高;新榛3号的单株根长、平均根粗和生根范围茎粗最高。IBA浓度为7.5 mmol/L时,新榛1号的单株根长、生根范围、单株新梢数、单株新梢长度和新梢饱满芽数均最高;新榛2号的生根率、生根指数和单株根数均最高;新榛3号的单株根粗和生根范围茎粗均最高。IBA浓度为10 mmol/L时,新榛1号的单株根长、生根范围和新梢饱满芽数均最高;新榛2号的单株根数最高;新榛3号的生根率、生根指数、平均根粗、生根范围茎粗、单株新梢数和单株新梢长度均最高。相关性分析得知,单株根数与单株根长,平均根粗与生根范围中部茎粗间的相关性均达极显著水平,相关性系数(R)分别为-0.897和0.911;生根范围与单株新梢数、单株新梢长度和新梢饱满芽数,生根范围茎粗与单株新梢长度间的相关性系数达显著水平,相关性系数分别为0.750、0.771、0.749和0.753。【结论】新榛1号黄化萌条在IBA速浸浓度为5 mmol/L处理后的性状综合表现较好(隶属函数平均得分值为0.64),新榛2号黄化萌条扦插在IBA速浸浓度为7.5 mmol/L处理后的性状综合表现较好(隶属函数平均得分值为0.73),新榛3号黄化萌条在IBA速浸浓度为10 mmol/L处理后的性状综合表现较好(隶属函数平均得分值为0.70)。     

不同浓度的吲哚丁酸和萘乙酸对中国沙棘雌株嫩枝扦插的影响

[目的]为快速扩繁中国沙棘雌株提供理论依据。[方法]以当年生半木质化生长、健壮、无病虫害的嫩枝为材料,以清水处理为对照,研究25、50、100mg/L吲哚丁酸（IBA）和25、50、100mg/L萘乙酸（NAA）处理对枝条成活率、株高、平均根数及平均根长的影响。[结果]IBA和NAA处理后的枝条成活率、株高、平均根数和平均根长均高于对照,不同浓度IBA处理的枝条成活率均高于NAA处理。50mg/LIBA处理的枝条成活率、成活枝条的株高、平均生根数、平均根长最高。[结论]IBA和NAA处理均可提高中国沙棘雌株扦插枝条的成活率、株高、平均根数和平均根长,其中50mg/L IBA处理效果最好。     

6-BA与IBA对长柄扁桃增殖的影响

植物激素是影响长柄扁桃增殖的一个重要因素。以长柄扁桃试管苗为材料,研究了不同质量浓度的6-BA与IBA组合对长柄扁桃增殖的影响。结果表明:MS+0.10mg/L6-BA+0.41mg/LIBA适于长柄扁桃的增殖,增殖倍数为4.1倍,平均苗高为1.50cm,试管苗生长健壮。     

Production of protocorm-like bodies with bioreactor and regeneration in vitro of Oncidium ‘Sugar Sweet’

To produce mass propagules of Oncidium ‘Sugar Sweet’, we tested the feasibility of producing protocorm-like bodies (PLBs) using 5 l balloon type air-lift bioreactors, and selected an optimal culture medium for shooting and rooting in vitro. The results showed that liquid bioreactor cultures were more efficient for PLBs proliferation when compared to solid- and liquid-agitated flask cultures. The maximum PLBs biomass (326.3 g per bioreactor, FW) was obtained in the immersion bioreactor culture, with the growth ratio reaching 10.9 after 50 days of culture. This was obviously higher than the ebb and flood bioreactor culture. During bioreactor culture, sucrose and electric conductivity (EC) in the culture medium were negatively correlated with PLBs growth; the highest PLBs fresh and dry biomass was obtained 40 days after culture. An inoculation density of 20 g (FW) was optimal for PLBs growth in a 3 l working volume of 5 l bioreactor. Furthermore, MS medium supplemented with 2.0 mg l⁻¹ BA for shooting and 0.5 mg l⁻¹ IBA for rooting was optimal during in vitro culture, the plantlets were successfully established in the potting substrate.     

Direct somatic embryogenesis and plant regeneration from leaf explants of Limoniumsinensis (Girard) Kuntze

The potentialities of direct somatic embryogenesis and plant regeneration from leaf explants of Limoniumsinensis var. Golden Diamond invitro were investigated. Young whole leaf and cut leaf explants when cultured on MS basal medium supplemented with each of the growth regulators N⁶-benzyladenine (BA) (0.44–2.2 μM) or thidiazuron (TDZ) (4.54 μM) alone or in combination with a fixed concentration of α-naphthalene acetic acid (NAA) (1.07 μM) produced somatic embryos directly. More than 90% of the leaf explants produced white, globular somatic embryos on BA (2.2 μM) and NAA (1.07 μM) supplemented MS basal medium within 1 week of inoculation. Most of the embryos matured further and converted after 8 weeks of culture on the same medium. Histological observation showed that the somatic embryos originated from single cells of epidermal layer of leaf. Histological evidence of formation of shoot and root poles during conversion of the embryos confirmed that these structures were true somatic embryos. After conversion the plantlets were further placed on MS medium containing 0.44 μM BA and 4.5 μM IBA for better shoot and root growth. About 90% of the plantlets transferred to the mixture of soil:perlite:vermiculite (1:1:1) in small plastic pots acclimatized successfully. Of these 85.5% plants survived after transferring into earthen pots containing a mixture of soil, coarse sand and cattle manure (1:1:1) under greenhouse or shady open condition.     

观赏羽衣甘蓝扦插繁殖初步研究

试验针对观赏羽衣甘蓝最佳扦插繁殖条件,研究分析了基质、激素对生根数量、生根速度、生根长度、茎高和新生芽数量的影响。结果表明:基质为珍珠岩+蛭石,激素为NAA 100mg/kg速蘸条件下,扦插效果最好;NAA在生根速度和生根数量上有优势,IBA在根长的生长上有优势;茎高和新生芽数量只能作为评价羽衣甘蓝扦插的参考指标;水做扦插基质不利于羽衣甘蓝生根。     

吲哚丁酸(IBA)纳米制剂对挪威槭绿枝扦插生根的影响

为提高挪威槭的繁殖效率,缩短其繁殖周期,以挪威槭1a生半木质化绿枝为材料,对其进行扦插试验,研究不同浓度的吲哚丁酸（IBA）原剂和吲哚丁酸（IBA）纳米制剂对挪威槭扦插成活率、生根率、平均根数、平均根长等指标的影响。结果表明：以浓度为150mg／L吲哚丁酸（IBA）纳米制剂处理的插穗生根效果最好。     

不同浓度的IBA、NAA和ABT对葫芦茶扦插效果的影响

采用当年生半木质化生长健壮的葫芦茶枝条为材料,以清水处理为对照,研究25、50、100 mg/L吲哚丁酸（IBA）、萘乙酸（NAA）和生根粉（ABT）处理对葫芦茶枝条成活率、平均生根数及平均根长的影响。结果表明：25 mg/L的ABT以及IBA和NAA各浓度处理的枝条成活率、平均根数和平均根长均显著（P〈0.05）高于对照,其中50 mg/L IBA处理效果最好,50 mg/L NAA次之。