Role of bioinoculants in development of salt-resistant saplings of Morus alba (var. sujanpuri) in vivo

Pot experiments were conducted for the development of salt-resistant saplings of Morus alba (var. sujanpuri) involving bioinoculants, namely arbuscular mycorrhizal (AM) fungi, Azotobacter and indole butyric acid (IBA). The IBA and sodium chloride (NaCl) concentrations were optimized prior to the experiments. By using both low and high concentrations, 15 ppm IBA and 0.05% NaCl (w/v) were found to be optimum in acting synergistically with AM fungi and Azotobacter and also for increasing all the growth parameters and microbial count in the rhizosphere. For in vivo development of salt-resistant saplings, the optimal concentration of IBA, along with AM fungi and Azotobacter in different combinations, was applied. The saplings were irrigated regularly with 0.05% NaCl water. Although growth parameters such as AM infection percentage, AM spores per 100 g soil and Azotobacter cells/g soil were affected by NaCl watering, the inoculation of both bioinoculants significantly enhanced survival percentage of saplings from 25 to 50% under salt stress. Maximum survival (55%) of saplings was found with IBA (15 ppm)+AM fungi+Azotobacter.     

IBA和PP_(333)对乌桕扦插生根的作用

用IBA500ppm和1000ppm酒精溶液处理乌桕嫩枝扦插条,能促进插条生根及生长。用500 ppm和1000ppm PP333溶液处理插条,扦插后的前20天表现出对生根有不同程度的抑制作用。但40天时,500 ppm处理的生根率明显增高,1000ppm处理的也出现了愈伤组织,并表明其对根的粗度生长具有促进作用。     

不同浓度IBA及基质对迷迭香扦插生根影响

用50 mg/L IBA、100 mg/L IBA、300 mg/L IBA浸泡迷迭香嫩枝插穗、半木质化插穗、完全木质化插穗30min后,扦插在沙子及混合基质中,均促进生根,成活率83%～100%,其中100mg/L IBA促进迷迭香插穗在沙子里及混合基质里生根效果最好,不同部位枝条插穗生根总体效果:半木质化枝条>嫩枝>完全木质化枝条。     

不同pH条件下IBA和NAA对伴矿景天生根的影响研究

为解决酸性环境中伴矿景天扦插苗生根难的问题,以河沙为基质,研究不同pH条件对伴矿景天扦插生根的影响,并进一步探究不同浓度吲哚丁酸IBA和萘乙酸NAA处理对酸性逆境环境下伴矿景天扦插苗生根的影响。结果表明：pH 5.5时,扦插苗虽能正常生长,但长势欠佳,当pH≤5.0时,植株根系发育不良,茎细短、叶片小,生物量不到正常生长(pH 6.5)时的40%,且pH越低植株死亡越快;用0.5~10.0 mg/L浓度的IBA和NAA溶液浸泡伴矿景天扦插苗可显著提高扦插苗成活率,主要表现为生根时间短、根系长、数量多。研究表明,pH≤5.0的扦插环境不利于伴矿景天扦插苗生长,扦插前使用浓度分别为2.0~3.0 mg/L和1.0~2.0 mg/L的IBA和NAA溶液浸泡伴矿景天扦插穗基部,可缩短扦插苗在酸性环境中生根时间、增加根系数量和根系长度,提高成活率。     

3种女贞属植物的扦插繁殖研究

对女贞(Ligustrum lucidum)、小叶女贞(L. quihoui)和金叶女贞(L. vicaryi)的扦插繁殖进行初步研究。结果表明:小叶女贞和金叶女贞的扦插生根率很高,不同处理组合之间差异相对较小;女贞的扦插生根率较低,不同处理组合之间的生根率相差达50%以上;对于女贞的扦插,选择适当的处理组合可显著提高其生根率,应用200 mg/kg NAA溶液浸泡长度为10 cm的女贞插穗20 s,可获得53.33%的生根率;金叶女贞扦插的最佳处理组合为:15 cm长的插穗,200 mg/kg的IBA溶液浸泡20 s;小叶女贞扦插的最佳处理组合为:15 cm长的插穗,200 mg/kg NAA溶液浸泡20 s。     

Significance of explant preparation and sizing in Aloe vera L.—A highly efficient method for in vitro multiple shoot induction

The present study was carried out to assess the effect of explant preparation and sizing for in vitro micropropagation of Aloe vera L. The stem nodal explants and shoot tips were cultured on modified Murashige and Skoog's medium (1962) supplemented with different concentrations of 6-benzylaminopurine (BA), kinetin (KIN), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and α-naphthaleneacetic acid (NAA) either singly or in combination. The best media composition was found to be MS medium supplemented with IAA (11.42 μM), IBA (9.8 μM) and BA (8.88 μM). The explants were divided into 2 sets, with and without ensheathing leaf base. Explant sizing, pruning and retention of mother tissue was highly significant in induction of multiple shoots and roots. The stem nodal explants with leaf base performed much better than those without such covering. A very high number of shoots and roots grew from these explants. The rooted plantlets were successfully acclimatized and transferred to the green house conditions and finally to field conditions.     

不同植物激素对条斑紫菜体细胞生长发育的影响

用添加不同浓度植物激素(IBA和6一BA)的PES培养液对条斑紫菜体细胞进行培养，观察其对条斑紫菜体细胞生长发育的影响。结果表明：酶解的条斑紫菜近基部体细胞离体培养可发育成细胞团和叶状体，低浓度的IBA有利于体细胞向叶状体方向发育，而低浓度的6一BA有利于体细胞发育成细胞团。     

袋鼠花水培扦插技术研究

扦插是袋鼠花繁殖的主要方法。本实验采用不同剂量营养液与吲哚丁酸（IBA）不同浓度相结合的方法,以清水为对照,通过测定袋鼠花的生根情况,探讨袋鼠花水培扦插的适宜处理组合。结果表明：营养液方面,清水处理对根条数、根长、根鲜重和生根率的影响明显优于其它处理,有利于袋鼠花的水培生根;IBA浓度为20mg/L时的生根率明显优于其它处理和对照,而当其浓度为40mg/L时对于根条数、根长和根鲜重则明显优于对照和其它处理,最有利于袋鼠花的水培生根。实验结果说明较低浓度的营养液（如清水）与中等浓度（如40mg/L）的IBA激素最有利于袋鼠花的水培生根。因此,本研究为缩短水培袋鼠花的生产周期及突出其观根特色,提高其观赏价值和商品价值提供理论依据。     

IBA与ABT组合对喜树插条内源激素变化的影响

采用酶联免疫法(ELISA),研究了不同浓度IBA与ABT组合对喜树嫩枝扦插生根效应及插条内源激素变化的影响。结果表明:与对照相比,IBA和ABT组合可显著提高叶及基部茎段内源激素IAA、ZR含量和IAA/ABA、ZR/ABA比值;降低GA3、ABA含量。进而提高喜树插穗成活率。各处理中,ABT 1 000+IBA1 000mg·L-1浸泡20s效果最好,处理后第40d插穗叶、茎内源IAA含量分别比对照增加57.5%、77.4%;叶内GA3含量第30d显著低于对照24.8%;茎段GA3含量第40d显著低于对照35.0%;叶、茎内ABA含量第30d时分别比对照降低19.2%、32.2%。叶、茎段内IAA/ABA第20、30d出现最高值,分别比对照提高114.0%、150.0%。叶、茎段内ZR/ABA变化与IAA/ABA变化类似,分别第20、30d达到峰值,较对照分别提高151.4%、39.9%。IAA/ABA、ZR/ABA可作为衡量喜树插穗成活质量的2个指标。     

不同质量浓度IBA对中药何首乌扦插的影响

以1年生硬枝为材料,研究不同IBA质量浓度(25,50,75,100,200mg/L)对何首乌扦插生根的影响。结果表明,不同质量浓度IBA对何首乌扦插枝条成活率、成活枝条的新生根数、根长、新发茎长度和新生叶片数均有显著影响;以IBA质量浓度为100mg/L的生根率最高,达48.5%;成活枝条新生根数最多,为2.5条;平均根长最长,为5.95cm;新发茎平均长度最长,为12.90cm;平均新生叶片数最多,达6.15片。说明用IBA处理何首乌扦插的适合质量浓度是100mg/L。