Effect of protein kinase C signal pathway on resistin expression in 3T3-L1 adipocytes

AIM：To investigate the effect of protein kinase C on resistin expression in 3T3-L1 adipocytes.METHODS：The differentiated 3T3-L1 adipocytes were incubated with 50 nmol/L phorbol 12-myristate 13-acetate (PMA) or 5 μmol/L Ro-31-8220 for 24 h.Expression of resistin mRNA was detected by RT-PCR and expression of resistin protein was detected by Western blotting.RESULTS：Compared with control,PMA increased the expression of resistin mRNA and protein in 3T3-L1 adipocytes significantly (P<0.01),while Ro-31-8220 decreased the expression of resistin mRNA and protein in 3T3-L1 adipocytes obviously (P<0.01).CONCLUSION：Protein kinase C signal pathway may regulate resistin expression in 3T3-L1 adipocytes.     

1-甲基环丙烯对高温下香蕉果实后熟的影响

以香蕉果实为试材,研究了1-甲基环丙烯(1-MCP)对高温逆境下香蕉果实后熟生理的影响。结果表明:35℃高温下贮藏的香蕉果实出现了明显的青皮熟现象,而0.5μL/L的1-MCP处理24h后可显著抑制高温下贮藏果实硬度的降低,延缓果皮细胞膜透性的升高,同时有效地延迟了果肉中淀粉酶活性升高、淀粉含量下降及可溶性糖含量上升。1-MCP处理果实于35℃下贮藏9d后移入20℃环境,进一步延缓了这些后熟生理变化。1-MCP和高温均抑制了果皮叶绿素含量下降,因此1-MCP处理减轻了香蕉热害的程度,延长了果实在高温下的贮藏寿命。     

丰蜜3号厚皮甜瓜的选育

丰蜜3号厚皮甜瓜是以自交系M3为母本、自交系0063为父本配制而成的短椭圆形厚皮甜瓜一代杂种。中早熟,果实发育期30-35d。单果重1.4kg左右,每667m2产量约2200kg。皮色浅黄,覆10条浅绿色纵沟,果面具细网纹;果肉白色,肉厚约3.5cm,肉质结实稍脆,味清香,果实中心可溶性固形物含量17%左右。     

Identification of Landscape Elements Related to Local Declines of a Boreal Grey-sided Vole Population

Several studies indicate a long-term decline in numbers of different species of voles in northern Fennoscandia. In boreal Sweden, the long-term decline is most pronounced in the grey-sided vole (Clethrionomys rufocanus). Altered forest landscape structure has been suggested as a possible cause of the decline. However, habitat responses of grey-sided voles at the landscape scale have never been studied. We analyzed such responses of this species in lowland forests in Västerbotten, northern Sweden. Cumulated spring densities representing 22 local time series from 1980–1999 were obtained by a landscape sampling design and were related to the surrounding landscape structure of 2.5×2.5 km plots centred on each of the 22 1-ha trapping plots. In accordance with general knowledge on local habitat preferences of grey-sided voles, our study supported the importance of habitat variables such as boulder fields and old-growth pine forest at the landscape scale. Densities were negatively related to clear cuts. Habitat associations were primarily those of landscape structure related to habitat fragmentation, distance between habitat patches and patch interspersion rather than habitat patch type quantity. Local densities of the grey-sided vole were positively and exponentially correlated with spatial contiguity (measured with the fragmentation index) of old-growth pine forest, indicating critical forest fragmentation thresholds. Our results indicate that altered land use might be involved in the long-term decline of the grey-sided vole in managed forest areas of Fennoscandia. We propose two further approaches to reveal and test responses of this species to changes in landscape structure.     

桃豫农矮化砧木1号的矮化机制

以豫农矮化砧木1号(Prunus persica L.cv.dwarfing rootstock Yunong1)、毛桃(P.persica L.)、寿星桃(P.persi-ca L.var.densa Mak.)、毛樱桃(Cerasus tomentosa Thunb.)和豫甜桃为试材,研究了豫农矮化砧木1号的矮化机制。结果表明,豫农矮化砧木1号的木质部导管长度、长宽比、叶片和韧皮部POD酶活性均居毛桃与寿星桃之间;叶片内源激素中生长素(IAA)的含量、细胞分裂素类与生长素的比值(ZR/IAA)均与豫农矮化砧木1号的矮化性状呈显著相关性;蛋白质双向电泳图谱显示豫农矮化砧木1号第9、13处有特异蛋白,而毛桃和寿星桃均没有;第10处蛋白质丰度有差异,依次是寿星桃>矮化砧木>毛桃;解剖结构显示豫农矮化砧木1号为砧木的嫁接部位愈合良好,且愈合部位出现4处漩涡纹。POD酶活性、内源激素的含量、导管和嫁接部位解剖结构的差异均是影响豫农矮化砧木1号矮化的因子。     

根癌农杆菌介导Cry1Ac基因转化‘雪青’梨获得转基因植株

 以‘雪青’梨叶片愈伤组织为外植体, 经根癌农杆菌介导将CaMV35S启动子调控下的Cry1Ac基因导入‘雪青’梨。698块愈伤组织和231个Kan^r芽与携带表达载体质粒pBX203的根癌农杆菌菌株LBA4404共培养3 d后, 转入含50 mg·L^-1 Kan的筛选培养基培养30 d, 15 d转接1次。结果表明,在MS + 5 mg·L ^-1 6-BA + 0.1 mg·L^-1 IAA筛选培养基中, Kan^r芽率为34.24% , 在1/2MS + 2 mg·L^-1 IBA+ 0.5 mg·L^-1 6-BA + 500 mg·L^-1AC筛选培养基中, 15.58% Kan^r芽生根。共获得再生植株32株, 经PCR和Southern-blot分析证明, 其中4株的基因组已成功导入和整合Cry1Ac基因。     

普通白菜(香青菜)黑杂-1号的选育

黑杂-1号普通白菜(香青菜)是利用不结球白菜Ogura雄性不育源黑不育1和自交系太黑香青菜配制而成的一代杂种。黑不育1的不育率、不育度均为100%,苗期和低温条件下不黄化,蜜腺发育正常,自然状态下能正常结实。黑杂-1号植株半直立,株型松散,株高30~35 cm,开展度50 cm,叶片椭圆形、深绿色、有光泽、全缘,叶面起皱不平,叶脉明显,叶柄绿白色、扁平,单株质量500 g左右,炒食香味浓郁;对病毒病、霜霉病和菌核病的抗性强于黑叶香青菜;每667 m2成株产量2 000 kg左右,小菜产量1 000 kg左右,适合江苏、上海、浙江、河北、安徽、山东、辽宁、四川等地推广种植。     

辣椒新品种淮椒3号的选育

淮椒3号是以南方引进的羊角形辣椒经3代定向选择而成的优良自交系95003为母本,以当地牛角形辣椒经4代单株自交育成的稳定自交系93010为父本配制而成的中晚熟辣椒一代杂种。定植至采收60d(天)左右,开花至始收30d(天)左右。植株生长势强,连续坐果能力强。果实粗羊角形,果长20cm左右,横径3～4cm,果肉厚2.5mm左右;果皮深绿色,光滑,肉质细软,微辣。对病毒病、炭疽病和疮痂病的抗性优于豫椒6号和八寸红。每667m2产量4000kg左右,适宜春露地、越夏、麦茬、麦套栽培,也可作秋延后栽培。     

拮抗细菌BMY-1菌株对几种水果储藏病原真菌的抑制作用

采用平板对峙培养法测定从土壤中分离获得的拮抗细菌BMY-1菌株对柑桔绿霉病菌（Penicillium digatatum）、柑桔青霉病菌（Penicillium italicum）、苹果炭疽病菌（Glomerella cingulata）、苹果轮纹病菌（Physalospora piricola）、梨扩展青霉病菌（Penicillium expansum）、葡萄白腐病菌（Coniothyrium dlodiella）等6种水果产后病原真菌的抑制作用。结果表明，BMY-1菌株对苹果炭疽病菌（G.cingulata）和梨扩展青霉菌（P．expansum）的生长有较好的作用，抑菌率分别达48．43％和50．37％。采用液体共培养法观察到BMY-1菌株对病原真菌的菌丝有破坏作用，使苹果炭疽病菌菌丝扭曲和断裂，葡萄白腐病菌菌丝膨大畸形。对几种水果同时接种拮抗细菌BMY-1和病原真菌，BMY-1菌株对苹果炭疽病病斑和梨扩青病病斑扩展有较强的抑制作用，并能有效延迟葡萄白腐病发病时间。     

早熟大白菜新品种琴萌8号的选育

琴萌8号是由自交不亲和系93-2-2SI和93-7-5SI配制而成的早熟秋大白菜一代杂种。生育期65d(天)左右,株高34.9cm,开展度80.8cm;外叶绿色,叶柄白绿色,叶球叠抱,倒卵圆形,结球紧实,球叶浅黄绿色,球高23.5cm,横径19.1cm,单球质量3kg左右,净菜率70%以上;高抗病毒病、霜霉病、黑斑病和软腐病,耐贮运,商品性好,VC含量179.80mg.kg-1(FW),可溶性总糖2.42%(DW),粗蛋白24.34%(DW);每667m2净菜产量5500kg左右,适宜山东、河北、北京、黑龙江、陕西、天津、河南等地早秋栽培,累计种植面积达6300hm2。