Effects of oxidized high-density lipoprotein on membrane fluidity and the expression of lymphocyte function associated antigen-1 of monocyte

AIM: To investigate the effects of oxidized high-density lipoprotein (oxHDL) on membrane fluidity and the expression of lymphocyte function associated antigen(LFA-1) of monocyte. METHODS: The membrane fluidity of THP-1 cells was assayed by fluorescence anisotropy with DPH (1,6-dipheny-1,3,5-hexatriene), a fluorescent probe; The LFA-1 expression on THP-1 cells were assayed by flow cytometry with indirect immunofluorescence.RESULTS: The membrane fluidity of THP-1 cells was reduced by 45% and 52% respectively (P＜0.01) after incubation with 100 μg protein/mL oxHDL and oxLDL for 20 h; oxHDL could stimulated the expression of LFA-1 on THP-1 cells, the positive cell rate and mean fluoresence intensity (MFI) increased by 39% and 45% respectively versus control group (P＜0.01) after incubation with cells for 24 h. CONCLUSIONS: By increasing the expression of LFA-1 on monocyte, oxHDL can promote the monocyte-endothelium adhesion; oxHDL also can reduce the membrane fluidity of monocyte, that will limit the returning of monocytes from subendothelium back to the circulation. This suggested that oxHDL may play an important role in atherogenesis.     

CYP450 epoxygenase/EET pathway attenuates adipose inflammation of obese mice through HIF-1α

AIM To investigate the effects of cytochrome P450 （CYP450） epoxygenase/epoxyeicosatrienoic acid （EET） pathway on insulin resistance in obese mice, and to explore the possible mechanisms. METHODS High-fat diet-induced obesity model was established in C57BL/6Cnc mice, and the obese mice were randomly divided into 3 groups, including obesity group （treated with saline; n=10）, EET group （treated with 11,12-EET; n=10） and EET inhibitor 14,15-epoxyeicosa-5（Z）-enoic acid （EEZE） group （n=10）. Normal C57BL/6Cnc mice （n=10） treated with saline served as control. Protein expression of CYP2J2 （one of CYP450 epoxygenases） and hypoxia-inducible factor-1α （HIF-1α） was measured by Western blot. Vessel-like structure was detected by immunofluorescence staining. The serum levels of insulin, tumor necrosis factor-α （TNF-α）, interleukin （IL）-1β, IL-6 and monocyte chemoattractant protein-1 （MCP-1） were measured by ELISA. RESULTS In obese mice, homeostasis model assessment of insulin resistance （HOMA-IR） values were increased, the protein level of CYP2J2 was reduced, and the protein level of HIF-1α was increased in adipose tissues as compared with the controls （P<0.05）. The serum levels of MCP-1, IL-1β, IL-6 and TNF-α were also significantly increased in obese mice （P<0.05）. After treatment with 11, 12-EET, the HOMA-IR values were decreased compared with vehicle-treated obese mice, HIF-1α expression levels were decreased in the adipose tissue, and the serum levels of MCP-1, IL-1β, IL-6 and TNF-α were reduced （P<0.05）. Immunohistochemical results of adipose tissue from vehicle-treated obese mice showed a marked decrease in vessel-like structures （CD31-positive） compared with normal control mice （P<0.05）. EET treatment significantly increased the newly formed vessel-like structures in the visceral adipose tissues of obese mice as compared with vehicle-treated obese mice （P<0.05）. CONCLUSION High-fat diet-induced obesity and insulin resistance are closely related to the CYP450 pathway. Exogenous EETs effectively decrease obesity-induced insulin resistance possibly through pro-angiogenesis and attenuation of hypoxia and inflammation.     

Progress in roles of MNX1 antisense RNA 1 in malignant tumor

MNX1 antisense RNA 1 （MNX1-AS1） is a newly discovered long non-coding RNA （lncRNA）, which is highly dysregulated in various carcinomas and its expression level is closely related to the overall survival and prognosis of patients. MNX1-AS1 regulates the occurrence and development of carcinomas by endogenous competitive adsorption of miRNA, regulating cell cycle, inducing epithelial mesenchymal transformation and activating multiple signaling pathways. The in-depth study of the carcinogenesis of MNX1-AS1 is useful for the early diagnosis, targeted therapy and prognostic assessment of relevant carcinomas. This article reviews the roles of MNX1-AS1 in malignant tumor.     

辣椒新品种辣优4号的选育

辣优 4号是以湖南地方品种分离株Ⅱ经多代自交筛选纯化的优良自交系 33号椒为母本、以材料h7 1经多代单株自交筛选的优良自交系 30号椒为父本配制而成的辣味型一代杂种 ,熟性早 ,前期产量比对照品种湘研 1号增产 16 .5%～ 4 9.1%。一般产量 16 0 0～ 2 80 0kg·(6 6 7m2 ) - 1。田间表现抗疫病、青枯病和病毒病。结果性好 ,单果质量 35～ 50g ,果实长牛角形 ,果表面光滑 ,色绿 ,商品率高 ,品质优良。现已在广东、广西、江西、海南、湖南等地推广种植 ,面积达80 0hm2 。     

辣椒新品种平椒3号的选育

平椒3号是以自交系99101为母本,自交系9949为父本配制的早熟一代杂种。果实羊角形,青熟果深绿色,果纵径17.7cm,果肩径3.0cm,果肉厚0.25cm,平均单果质量29.5g,肉质脆嫩,辣味浓且有香味,商品性好。田间对疫病、炭疽病、病毒病的抗性明显优于猪大肠。露地栽培每667m2青椒产量为3700kg左右,适宜西北地区及四川、山西、湖南等嗜辣地区露地栽培。     

辣椒新品种川腾2号的选育

川腾2号辣椒是以自交系03-8-4-2作母本,以自交系03-9-4-6作父本配制而成的一代杂种。该品种极早熟;果实粗牛角形,纵径12.2cm,横径4.5cm,果肉厚0.4cm,单果质量53.2g;青熟果深绿色,老熟果红色,外观商品性好;肉质脆嫩,微辣,适鲜食。露地667m2产鲜椒3000kg左右。耐寒、耐热、耐旱、耐涝,抗病毒病、疫病、炭疽病、蚜虫、跗线螨、烟青虫等的能力超过湘研1号,与湘研11号相当,为强或较强,适合四川和全国种植。     

超级杂交稻国稻1号农艺性状研究

对超级杂交稻国稻1号和杂交稻汕优63的株型特征以及农艺性状进行了比较分析,结果表明:国稻1号的单产之所以显著高于汕优63的,主要因为前者分蘖多且分蘖快,单位面积有效穗数和每穗总粒数多,而且结实率高。     

Progress in Isoindolone Alkaloid Derivatives from Marine Microorganism: Pharmacology,Preparation, and Mechanism

Compound 1 (SMTP-7, also FGFC1), an isoindolone alkaloid from marine fungi Starchbotrys longispora FG216 and fungi Stachybotrys microspora IFO 30018, possessed diverse bioactivities such as thrombolysis, anti-inflammatory and anti-oxidative properties, and so on. It may be widely used for the treatment of various diseases, including cerebral infarction, stroke, ischemia/reperfusion damage, acute kidney injury, etc. Especially in cerebral infarction, compound 1 could reduce hemorrhagic transformation along with thrombolytic therapy, as the traditional therapies are accompanied with bleeding risks. In the latest studies, compound 1 selectively inhibited the growth of NSCLC cells with EGFR mutation, thus demonstrating its excellent anti-cancer activity. Herein, we summarized pharmacological activities, preparation of staplabin congeners—especially compound 1—and the mechanism of compound 1, with potential therapeutic applications.     

OVER- EXPRESSION OF AN AUX1 HOMOLOG TRIGGERS OVARY INITIATION IN VITRO OF POPULUS TOMENTOSA CARR.

INTRODUCTIONPolar transport is one key feature of auxin action was believed as a positional signal plays a wide arrange ofroles in plantgrowth and development,such as the gravity response[1],the initiation of lateral roots[2],positiondetermination of leaf primordial[3,4],differentiation of flower primordial[5],the axis formation in developingembryos[6~8],the patterned differentiation of vascular tissues[9~11].Polar auxin transport(PAT)system contributes indole-3-acetic acid(IAA)thorough …     

不同区域及栽培密度下耐密植芝麻新品种豫黑芝1号产量及产量相关性状变化分析

芝麻是我国重要的特色优质油料作物。提高芝麻单产水平是实现我国粮油安全生产的重要举措。为建立芝麻高产高效栽培模式,本研究以耐密植黑芝麻新品种豫黑芝1号为试验材料,在3个产区(漯河、信阳和三门峡),开展了不同栽培密度(12万株/hm2、15万株/hm2、18万株/hm2、21万株/hm2)下豫黑芝1号产量及产量相关性状变化分析。结果显示,豫黑芝1号在不同产区的生育期差异明显;种植密度对豫黑芝1号的株高、有效果节数、主茎果轴长、单株蒴果数、千粒重和单株产量等性状变化程度不同;对小区产量及单株产量影响显著。在21万株/hm2密度下,豫黑芝1号单产水平最高达到1463.33 kg/hm2(三门峡)。研究为芝麻高产高效生产提供了技术支持。