目前我国兽药质量状况及提高兽药质量的对策

分析了我国兽药产品质量状况 ,探讨了社会因素、生产过程以及流通、使用环节、质量监督等方面对兽药产品质量的影响 ,提出了提高我国兽药产品质量的对策和方法。着重阐述了法制建设、队伍建设 ;提高从业人员素质 ;全面实施兽药生产质量管理规范 (GMP) ;流通、使用环节的质量保证 ;以及完善检验标准、加大监控力度等对提高兽药质量的重要意义     

兽药GMP验证中的制药用水系统

全面介绍了兽药GMP验证中的制药用水系统。     

企业实施GMP后兽药市场竞争与发展方略

论述企业实施GMP后国内商品兽药的质量属性、生产现状、资源利用、发展品种、市场竞争形式及其发展方略.     

The origin and early development of wheat glutenin particles

Breadmaking quality is strongly related to the glutenin macropolymer (GMP) fraction. Don and co-workers [Don, C., Lichtendonk, W.J., Plijter, J.J., Hamer, R.J., 2003a. Glutenin macropolymer: a gel formed by particles. Journal of Cereal Science 37, 1–7] showed that GMP consists of spherical glutenin particles and suggested that these originate from the protein bodies (PBs) observed in developing grain. We have tested this hypothesis by systematically comparing SDS-soluble and SDS-insoluble protein fractions from both PBs and flour. These preparations were analysed at the molecular, oligomer, particle and microscopic levels. Comparison of PBs isolated from immature seeds with glutenin particles isolated from mature seeds revealed strong similarities in protein composition and the presence of large glutenin oligomers. However, the glutenin particles from mature wheat were significantly larger than PBs. We suggest that PBs are the building blocks for the formation of much larger glutenin particles which are formed during the desiccation phase of kernel development.     

喷施ABA对小麦籽粒谷蛋白组分含量及GMP粒度分布的影响#br#

【目的】探讨喷施外源激素ABA引起小麦籽粒谷蛋白组分及谷蛋白大聚合体(glutenin macro-polymer, GMP)粒度分布的变化,为激素调控改善小麦籽粒品质提供理论依据。【方法】以冬小麦品种山农8355和山农15为试验材料,通过大田试验研究喷施外源ABA对小麦籽粒谷蛋白组分含量及GMP粒度分布的影响。【结果】在孕穗末期和籽粒形成期喷施ABA(浓度12 mg&#8226;kg-1,用量45 mL&#8226;m-2)均能提高籽粒粗蛋白及谷蛋白含量,同时,籽粒中高、低分子量不溶性谷蛋白含量均有所增加。特别是在孕穗期喷施ABA,山农8355和山农15两品种籽粒中高、低分子量不溶性谷蛋白含量与对照相比均达到显著水平,但喷施ABA对籽粒中可溶性谷蛋白含量影响相对较小。不论是孕穗期还是籽粒形成期喷施ABA均能增加GMP含量与产量,并改变籽粒中GMP的粒度分布,尤其是对其体积及表面积分布影响相对较大。相关分析表明,高、低分子量不溶性谷蛋白、GMP含量及GMP/Pr.比值均与GMP小颗粒(d<15 um)粒度分布呈现负相关,与大颗粒(d≥15 um)粒度分布的关系呈正相关。【结论】 喷施外源ABA可以改变籽粒中谷蛋白组分及GMP粒度分布,从而影响小麦籽粒品质。     

玻璃化冷冻对猪MII期卵母细胞发育能力的影响

以MII期卵母细胞作为试验材料,应用玻璃化冷冻方法,就目前应用最多的7组冷冻保护液配方进行筛选,并且比较3种不同冷冻载体冷冻卵母细胞的效果。结果发现:7组冷冻保护液对MII期卵母细胞有低毒性作用,各组分裂率、囊胚率、囊胚细胞数均低于对照组;综合各组指标,选取3组对猪MII期卵母细胞的发育能力损伤最小的冷冻保护剂,应用GMP管对其冷冻保护效果进行比较,发现第7组冷冻保护液配方EFS不适于MII期卵母细胞的冷冻,其形态正常率、分裂率分别为1.19%、0,与第3组、第6组形态正常率、分裂率(59.48%、53.55%;24.19%、35.02%)差异显著(P0.05)。综合考虑,选取HM+7.5%(DMSO+EG),HM+17%(DMSO+EG)+0.4 mol/L Su作为冷冻保护剂来比较3种不同冷冻载体冷冻卵母细胞的效果差异,发现半麦管冻融后MII期卵母细胞的分裂率高达53.67%,与OPS法的29.33%及GMP法的35.00%相比差异显著(P0.05),半麦管法更适合MII期猪卵母细胞的玻璃化冷冻。     

Correlation of glutenin macropolymer with viscoelastic properties during dough mixing

Although significant correlations exist for glutenin macropolymer (GMP) quantity and rheological properties/bread making quality of dough, little information about these links is available. The relationship between GMP contents measured by UV absorption method/RP-HPLC and dough viscoelastic properties determined by TA-XT2i texturometer from three wheat varieties (Xiaoyan6, Yumai56 and Zhengnong8805) during mixing was investigated. GMP contents of doughs decrease significantly (P<0.05) during mixing. During the initial mixing stage, amounts of the HMW-GS and LMW-GS and GMP decrease significantly (P<0.05). Their contents begin to increase beyond peak dough development time (DDT). This indicates that during further mixing after peak DDT some glutenin subunits are incorporated into GMP by repolymerization. The HMW/LMW-GS ratio has a significant effect on load-deformation properties (area, resistance and extensibility) of dough. The varieties behaved differently in relation to the contribution of their HMW-LMW-GS ratio to the rheological properties.     

甘肃省兽药行业发展现状评价与对策

动物药品作为动物疫病防控的有力武器和养殖业增产的重要手段,是养殖业、快速健康、持续发展的不可或缺的重要保障。我国动物药品业现已基本实现了产品从单一化向多元化的转化,并出现了从生产型、数量型向效率型、质量型的变化。随着企业规模不断扩大、效率不断提高,产品结构不断升级,动物药品已经由需求、质量层面向强调安全和科学发展过渡。针对当前我国兽药行业的现状以及甘肃省兽药行业发展中存在的问题,报道了目前甘肃省兽药生产企业的发展状况,并对存在的主要问题进行了探讨和分析,结合甘肃省实际情况和兽药行业的发展趋势,进一步对甘肃省今后兽药研制发展的目标和重点进行了探讨。     

制约兽药GMP实施的因素与对策

实施兽药GMP已到了攻尖克难阶段.笔者分析了兽药质量管理的现状,剖析了加速推行兽药GMP的制约因素,从投入、政策、服务、外资和市场等五个方面寻找应对之策.     

Experiment on Cryopreservation of Porcine Oocytes by Using Glass Micropipette

用自制的玻璃微管（glass micropipette，GMP）冷冻猪卵母细胞。以FDA染色鉴定冷冻-解冻后卵母细胞存活力，结果表明，用常规细管程序化冷冻和GMP管玻璃化冷冻猪成熟（MⅡ期）卵母细胞时，冻后存活率分别为34.5%和63.3%（P <0.05）；以玻璃化冷冻比较细管和GMP管对猪MⅡ期卵母细胞冷冻效果的影响，两组冻后存活率分别为45.0%和65.9%（P < 0.05）；用常规细管程序化和GMP管玻璃化冷冻猪GV期卵母细胞时，冻后存活率为30.0%对59.7%，有显著性差异（P < 0.05）。研究表明，GMP法为一种有效的猪卵母细胞冷冻保存方法。