Experimental Research on the Evolution of Hysteresis Curve of Rock in Different Axial Stress Levels

对天然杀菌剂茉莉酮酸酯、芥子油苷、蜂胶、醋酸瓜类萎蔫醇、壳聚糖、香精油剂等控制果蔬采后腐烂的方法与作用机制进行调研,研究结果表明,天然杀菌剂具有较好的抗菌作用.     

南方红壤区植被覆盖因子估算模型构建与验证

研究红壤区植被覆盖因子变化规律、构建植被覆盖因子计算模型可为该区域水土流失防治及动态监测提供科学依据。该研究基于广东省五华县水土保持试验推广站200余场降雨试验观测数据,分析了影响水土流失的各关键因子与次降雨土壤侵蚀模数(Individual Rainfall Event Soil Erosion Modulus,ISEM)间定量关系,基于中国土壤流失方程计算了系列次降雨事件不同植被覆盖度对应植被覆盖因子值,构建了植被覆盖因子值与植被覆盖度间数学模型,从点、面2个尺度对模型计算精度进行了验证。结果表明:1)ISEM随植被覆盖度增加而降低;ISEM与雨前土壤表层含水率间存在显著正相关对数关系;ISEM随坡度增加呈先增后减的变化规律;ISEM随次降雨量、次降雨侵蚀力和次降雨径流深呈显著正相关线性变化关系。2)构建了植被覆盖因子值与植被覆盖度间二阶指数衰减模型,该模型决定系数和纳什系数分别达0.947和0.876。点尺度验证结果表明90%样本模型计算值与观测值相对误差均小于0.30;面尺度验证结果表明,70%～80%的植被覆盖因子计算值相对误差不超过0.1。总体而言,该模型计算精度较为理想,但由于研究对象典型的时空尺度特征,仍需要更多观测数据对该模型进行完善和验证。研究成果可为深入理解红壤区土壤侵蚀规律、水土流失动态监测提供有益参考。     

樟子松热处理材耐久性能的评价

对樟子松热处理材进行室内防霉、防蚁蛀及野外埋地检测,综合评价其耐久性能。结果表明:樟子松热处理材对霉菌、变色菌均无防治效力,不抗白蚁蛀蚀;樟子松热处理材的耐久年限约为3年。     

Tree cavities in forests - The broad distribution pattern of a keystone structure for biodiversity

We explored the main factors affecting the global distribution of tree cavities - a habitat component of mostly biotic origin that is crucial for many animal species. We considered the influence of eight environmental variables (ranging from the single-tree to the biogeographic-region scale) on cavity density in a meta-analysis of 103 published studies. The global median density of cavities was 16 ha⁻¹, with densities highest in Australasia and lowest in the Palaearctic region. Two major factors influencing density were identified: cavity density was positively related to the amount of precipitation, and was higher in natural than in managed forests. These effects suggest that the distribution of tree cavities largely reflects the incidence of fungal heart-rot in trees, and that forest management, by affecting wood decay processes, can have a broad-scale impact on tree microhabitat availability. Although air temperature, forest composition and wood hardness had suggestive univariate effects, neither these variables nor biogeographic region explained any additional variation in multifactor models. In regions where woodpeckers are present there was an upper limit to the density of woodpecker-excavated cavities (approximately 10-20 cavities ha⁻¹) that was considerably lower than the highest total cavity densities encountered (up to 140 ha⁻¹). This indicates that primary cavity-nesters are particularly important keystone species in cavity-poor forests where wood decay processes are suppressed either climatically or by forest management.     

Fatty acid composition of various ectomycorrhizal fungi and ectomycorrhizas of Norway spruce

Whole cell fatty acid (WCFA) compositions of three different structures of ectomycorrhizal (ECM) fungi: sporocarps, pure culture mycelia and ectomycorrhizas were analysed to evaluate the potential use of fatty acid profiles as biomarkers for ECM fungi and ectomycorrhiza-associated bacteria. Sporocarps of Amanita muscaria, Amanita rubescens, Lactarius rufus, Lactarius thejogalus, Leccinum scabrum, Paxillus involutus, Russula foetens, Russula rosea, Russula vesca, Suillus grevillei, Tylopilus felleus, Xerocomus badius, Xerocomus subtomentosus, pure cultures of A. muscaria, P. involutus, X. badius, X. subtomentosus, Suillus bovinus Suillus luteus and seven ectomycorrhizal morphotypes of Norway spruce were examined. Our results revealed species-specific composition of fatty acids of fungal sporocarps and pure culture mycelia. Ectomycorrhizal morphotypes distinguished and identified by morphological and molecular methods (PCR-RLFP and sequencing) created specific fatty acid profiles. The dominating fatty acids in pure cultures and sporocarps were 18:2ω6,9, 18:1ω9 and 16:0, whereas ectomycorrhizas also contained plant and bacterial specific fatty acids. Especially, fatty acids specific to Gram-positive bacteria 15:0 anteiso and 17:0 anteiso were present in relatively high amounts and suggested that these bacteria are dominating in the examined Norway spruce mycorrhizosphere. In conclusion, our results show that fatty acid based methods can be useful in studies of ectomycorrhizal fungi, both as a quick method for differentiation of fungal species and also in studies of mycorrhiza-associated microorganisms in the field.     

Spatial changes of soil fungal and bacterial biomass from a sub-alpine coniferous forest to grassland in a humid, sub-tropical region

 Fungal and bacterial biomass were determined across a gradient from a forest to grassland in a sub-alpine region in central Taiwan. The respiration-inhibition and ergosterol methods for the evaluation of the microbial biomass were compared. Soil fungal and bacterial biomass both significantly decreased (P<0.05) with the shift of vegetation from forest to grassland. Fungal and bacterial respiration rates (evolved CO₂) were, respectively, 89.1 μl CO₂ g^–1 soil h^–1 and 55.1 μl CO₂ g^–1 soil h^–1 in the forest and 36.7 μl CO₂ g^–1 soil h^–1 and 35.7 μl CO₂ g^–1 soil h^–1 in the grassland surface soils (0–10 cm). The fungal ergosterol content in the surface soil decreased from the forest zone (108 μg g^–1) to the grassland zone (15.9 μg g^–1). A good correlation (R ²=0.90) was exhibited between the soil fungal ergosterol content and soil fungal CO₂ production (respiration) for all sampling sites. For the forest and grassland soil profiles, microbial biomass (respiration and ergosterol) declined dramatically with depth, ten- to 100-fold from the surface organic horizon to the deepest mineral horizon. With respect to fungal to bacterial ratios for the surface soil (0–10 cm), the forest zone had a significantly (P<0.05) higher ratio (1.65) than the grassland zone (1.05). However, there was no fungal to bacterial ratio trend from the surface horizon to the deeper mineral horizons of the soil profiles. Received: 30 March 2000     

Novel antibiotics as inhibitors for the selective respiratory inhibition method of measuring fungal:bacterial ratios in soil

The use of the selective inhibition (SI) method for measuring fungal:bacterial ratios may be limited due to biocide selectivity and the overlap of antibiotic activity. This study evaluated novel pairs of antibiotics for their specificity in soils of different origins and their potential reduction in inhibition of non-target organisms. Four soils selected for this study were from a semi-arid shrub-steppe, a loblolly pine forest and two grassland sites (restored and farmed prairie plots). Three bactericides were tested: oxytetracycline hydrochloride, streptomycin sulphate, and bronopol. Three fungicides were tested: captan, ketoconazole, and nystatin. The inhibitor additivity ratio and fungal:bacterial ratios were calculated from control and treated soils where inhibition was measured as CO₂ respiration reduction with biocides. We were able to minimize non-target inhibition by the antibiotics to <5% and thus calculate reliable fungal:bacterial ratios using captan to inhibit fungi in all four soils, and bronopol to inhibit bacteria in three of the four soils. The most successful bactericide in the restored prairie was oxytetracycline-HCl. Our results demonstrate that application of novel antibiotics is not uniformly successful in soils of different origin and that the SI technique requires more than just optimization of antibiotic concentration; it also requires optimization of antibiotic selection.     

Mechanisms of stabilization of earthworm casts and artificial casts

Summary Fresh casts were collected from the earthworm species Aporrectodea caliginosa, and artificial casts were also made. The casts were subjected to ageing, drying-rewetting, and sterilization by hexanol vapour. Clay dispersion was determined, as a measure of the lack of stability of the casts. Two soils were used, the topsoil of a recently reclaimed polder soil in the Netherlands and the topsoil from a South Australian duplex soil. For both soils the fresh worm casts had higher dispersible clay than the artificial casts. During ageing, both types of casts became more stable. There are strong indications that this was mainly due to changes on the surface of the casts. Fungi developed on the surface of 6-day-old worm casts made of Australian soil. This gave a higher stability to the casts compared to artificial casts of the same age without fungal growth. With both types of casts, hexanol inhibited fungal growth on the surface of the casts, reducing the stabilizing effect of ageing. The fungus did not develop on Dutch soil casts until after 42 days, and the development of a higher stability with age was also less marked than in the Australian soil. When the casts were subjected to a drying and rewetting cycle before analysis, they became much more stable than the casts that were analyzed wet. The drying-rewetting cycle removed most of the differences between the worm casts and the artificial casts, and also removed any effects of ageing.Dedicated to the late Prof. Dr. W. Kühnelt     

储烟病害研究及其进展

综述了对储烟病害特别是储烟霉变的研究。霉变是由多因子引起的,贮烟的含水率、库内温度、湿度以及贮烟管理水平都直接影响到烟叶霉变的发生和程度的轻重,因而可根据烟叶霉变的机理对其进行综合治理。     

黄皮葫芦果实贮藏保鲜试验

对黄皮葫芦果实进行了4种温度处理,7种保鲜剂处理的贮藏试验,结果表明:黄皮葫芦果实对低温敏感,低于8 ℃会发生冷害。温度越低,冷害越严重,主要冷害症状为瓜皮黄色消褪、进而褐变或黑变。0 ℃、6 ℃下单果包装黄皮葫芦果实的安全贮藏期为3 d,7～8℃下为6 d。保鲜剂能显著提高黄皮葫芦果实的鲜度,且能显著抑制瓜把的腐烂或延迟瓜把腐烂期的到来,较CK的贮藏期分别延长9 d和18 d。