十字花科蔬菜上黄曲条跳甲种群动态调查与分析

1996～1997年在深圳对黄曲条跳甲种群动态调查的结果表明 :该虫在一年中有两个发生高峰 ,即春季高峰和秋季高峰。秋季发生高峰的虫口数量是春季的2.4～2.5倍。旬降雨量 (X₁)、旬平均气温 (X₂)与黄曲条跳甲种群数量 (Y)之间的关系用数学方程式表达 :Y=27.1537-0.004 128X₁+0.004727X₂。黄曲条跳甲在一季菜生长期内的发生动态与寄主植物生长发育期密切相关。本文还讨论了黄曲条跳甲在不同寄主植物上的种群动态     

Progress on role of CCN1 in pulmonary diseases

Cysteine-rich angiogenic inducer 61 (Cyr61/CCN1) is an extracellular matrix-associated signaling protein consisting of 381 amino-acid residues, which has the regulatory function for a multitude of cellular responses. The pleiotropic effects of CCN1 on the initiation and resolution of inflammation as well as oncogenesis and development of tumor were reported. According to the numerous data from experimental and clinical studies, this article provides an overview on CCN1 and summarizes the latest understanding of the role of CCN1 in pulmonary diseases.     

新隆优一号在皖南地区高产制种技术研究

介绍了宣城市的自然气候特征以及新隆优一号亲本特征与特性，分析了高产制种技术要点。     

Fumonisin B1 Metabolism by Bovine Liver Microsomes

Only limited and contrasting information is available about the metabolic fate in cattle of fumonisin B₁, a mycotoxin produced by moulds of Fusarium. This study was carried out to evaluate the hepatic metabolism of fumonisin B₁ by bovine liver microsomes. No biodegradation or metabolization of the mycotoxin by liver microsomes was detectable after incubating fumonisin B₁ with bovine microsomes in the presence of a regenerating system for 1 h. No aminopolyol 1, aminopolyol 2 or aminopentol, metabolites of fumonisin B₁, were detected in any of the incubated samples. The tolerance of ruminants to fumonisin B₁ is apparently not dependent on its detoxification in the rumen.     

应用逆转录套式PCR检测新城疫病毒核酸

选择DNV融合蛋白保守的编码区域,设计并合成了一对外引物和一对内引物,建立并优化了检测新城疫病毒核酸的逆转录套式PCRI地,通过检测NDV感染的实验客观存在病料和临床病料,结果表明,逆转录套式PCR法最低能鉴别出约0.3pg的NDV RNA,攻毒后第8天还能从非免疫鸡和SPF鸡泄殖腔拭子中检出DNV,第8天非免疫鸡泄殖腔拭子中NDV的最大检出率为5/10,第8天SPF鸡泄殖腔拭子中NDV的最大检出率为6/10,对非免疫鸡和SPF鸡的泄殖腔中NDV最佳检出时间均在攻毒后第5天。逆转录套式PCRY应运地临床样品中DNV的最大检出率为6/7,经核酸杂交验证,该法具有很高的特异性和敏感性,也比较简便快速,为从分子水平探讨NDV的发病机理、临床早期快速诊断提供了新的研究手段。     

Identification of Trichoderma SKT-1, a biological control agent against seedborne pathogens of rice

Trichoderma SKT-1 was previously reported as a powerful biological control agent against seedborne pathogens of rice, but the taxonomic disposition of the fungal isolate was not clear. Trichoderma SKT-1 produced irregular pyramidal warts on conidia and had an optimum growth temperature of 30°C. Morphological characteristics and colony growth were identical to those of known species of Trichoderma, including the newly recognized species T. asperellum. The 5.8S rDNA with the internal transcribed spacer (ITS) region (ca. 514 bp) of the fungus was compared with those of known species to determine the phylogenetic placement of the fungus. The length and sequence of the regions from Trichoderma SKT-1 were completely identical to those of an isolate of T. asperellum NRRL 5242 (AJ230669). On the basis of these results, we concluded that Trichoderma SKT-1 was T. asperellum.     

O型口蹄疫病毒VP1嵌合基因的构建及原核表达

首先合成我国O型口蹄疫病毒2个疫苗毒株VP1基因的3个抗原袁位，与另外扩增的流行毒株VP1基因末端273bp片段相连,构建出O型VP1嵌合基因片段（VP1O）。然后，将VPIO基因连接到原核表达载体pET28a上，构建了重组表达质粒pET28a-VP1O，转化大肠杆菌BL21（DE3）进行诱导表达。SDS-PAGE电泳表明。VP1O基因在大肠杆菌中获得表达。Western blotting检测证实表达的VP1O蛋白具有良好的生物学活性。对表达蛋白通过包涵体洗涤的方法进行初步纯化，获得了较高纯度的VP1O蛋白。     

胸腺素α_1对墟岗黄鸡免疫器官组织学结构的影响

本试验研究了不同剂量的胸腺素α1(Tα1)对墟岗黄母鸡免疫器官指数和组织学结构的影响。结果表明:0.015mg/kg体重剂量的Tα1可显著提高鸡免疫器官指数,可使胸腺皮质增宽,髓质胸腺小体数和胸腺细胞数增加;脾脏胸腺依赖区和法氏囊依赖区细胞增殖;高剂量Tα1则引起法氏囊髓质局部细胞变性和坏死。     

“难分离有机磷杀虫剂对”的毛细管气相色谱法分析研究

研究了用3种大口径毛细管柱分析由有机磷杀虫剂组成的“难分离有机磷对”。HP－1大口径毛细管柱只能分离由甲基对硫磷和其它有机磷杀虫剂组成的“有机磷对”；QF－1大口径毛细管柱能够分离部分“有机磷对”；阿皮松L固定液大口径毛细管柱能完全分离马拉硫磷和水胺硫磷组成的“有机磷对”。     

Conditional expression of harpinPsscauses yeast cell death that shares features of cell death pathway with harpinPss-mediated plant hypersensitive response (HR)

Conditional expression of harpin_Psscauses yeast cell death that shares features of cell death pathway with harpin_Pss-mediated plant hypersensitive response (HR).Pseudomonas syringae pv.syringae 61 hrp Z gene encodes harpin_Pss, a 34.7 kD extracellular protein that elicits a hypersensitive response (HR) in plants. Conditional expression of either full-length or truncated hrp Z sequences under the GAL1 promoter caused cell death in Saccharomyces cerevisiae Y187. Plating of pYEUT- hrp Z transformants on a medium containing galactose resulted in complete inhibition of colony formation, whereas their growth on a glucose-based medium was unaffected. Western blot analysis confirmed the expression of harpin_Pssin yeast cells transformed with pYEUT- hrp Z and grown in galactose-containing medium. A time-dependent decline in the percentage of trypan blue-excluding cells in cultures of pYEUT- hrp Z transformants was observed when cultured on galactose-containing medium. Similarly, the number of viable cells reduced to about 50% within 6 h. There were similarities in the harpin_Pss-mediated cell death in plants and yeast cell death (YCD). Galactose-induced cell death in pYEUT-hrp Z transformants of S. cerevisiae Y187 was suppressed by a protein kinase inhibitor K252a (10 μ M). The viability of pYEUT- hrp Z transformants was prolonged in the presence of 100 U ml⁻¹catalase suggesting a role for the oxidative burst in YCD that was further supported by the flow cytometric patterns of propidium iodide uptake by yeast cells. Overall, it appears that yeast provides a useful model system to understand the molecular mechanism of harpin_Pss-mediated cell death.