茶树咖啡碱合成酶CRISPR/Cas9基因组编辑载体的构建

CRISPR/Cas9技术是一门新兴的基因组定点编辑技术,具有操作简单、高效的优点,可轻松实现对目标基因的敲除、替换和定点突变等操作。该技术刚诞生,就受到了全球生命科学领域研究者的关注,不到3年的时间就已经成功应用于多种动、植物当中。然而CRISPR/Cas9技术在茶树中的应用面临载体构建问题,本文以茶树咖啡碱合成酶为例,联合采用常规PCR、Overlapping PCR和Golden Gate Cloning技术,构建了包含茶树咖啡碱合成酶双靶点的CRISPR/Cas9基因编辑载体,为CRISPR/Cas9介导的基因组编辑技术在茶树中的应用奠定了坚实基础。     

莱豆新品种龙莱1号的选育

征集龙岩市莱豆地方品种13个,按照育种目标进行品种筛选、提纯、繁殖、特征特性观察与栽培技术试验,育成的莱豆新品种龙莱1号具有中熟、品质优、产量高、抗病性较强、适应性广、商品价值高等特点。该文介绍该品种从2003～2010年的选育过程及该品种主要特征特性。     

Breeding Report of New Mung Bean Variety“Ji Heilv No.12”

The mung bean variety Ji Heilv No.12 was bred by lnstitute of Characteristic Crop Research, Chongqing Academy of Agricultural Sciences and lnstitute of Food and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences. using Jilv 9 and Jilv 7 as female and male parent respectively,with pedigree method. Ji Heilv No.12 is a new variety with features of high and stable yield,broad adaptability and strong resistance in yield trails during 2011-2012; it was approved and released by Chongqing Provincial Committee of Crop Variety ldentification in 2012,suitable for cultivating in most area of Chongqing.     

稻-草-鹅种养模式的应用价值

稻-草-鹅种养模式是一种生态种养模式，具有较好的生态效益、经济效益和社会效益。文章就稻-草-鹅种养模式的含义、生态效益、经济效益和社会效益、应用价值作一综述，以期为稻-草-鹅种养模式的广泛推广应用提供理论依据。     

生物技术在农业领域的应用

生物技术包括基因工程、细胞工程、酶工程等,在农业领域有着广阔发展前景.生物技术可保障粮食安全,促进农村农业经济的发展,提高农业产出效益,同时能为医药工业及其它产业提到原料.农业生物技术是新兴的现代技术,还不能在传统农业中大规模推广应用.     

高寒半干旱地区4种牧草的适应性及坪用价值

[目的]通过冷地早熟禾（Poa crymophila）、中华羊茅（Festuca sinensis cv.）、星星草（Puccinella tenuiflora）和野生早熟禾（wild Poa pratensis）4种小草籽的引进繁育,观察小草籽品种的适应性和坪用性,筛选出抗逆性强、管理粗放、适应城市绿化栽植的人工草坪地植被品种,为品种资源的开发打下良好基础。[方法]2009年在青海省共和盆地木格滩高寒半干旱地区种植牧草田,2010年观察牧草各品种的越冬性能、生长习性、适用性和坪用性。[结果]4种牧草品种越冬率均在95.0%以上,表现出耐寒、越冬性极强的特点。中华羊茅生育期最长,其次是冷地早熟禾,野生早熟禾和星星草生育期最短。持续高温干旱天气对中华羊茅生长发育有明显影响,而对其他3种牧草的影响较小。4种牧草品种的实际草籽产量顺序依次为中华羊茅〉野生早熟禾〉冷地早熟禾〉星星草。中华羊茅产草量和地上生物产量比星星草、冷地早熟禾和野生早熟禾高。种子产量中,中华羊茅最大,其次是星星草,冷地早熟禾和野生早熟禾种子产量相同,最小。[结论]中华羊茅、星星草、冷地早熟禾和野生早熟禾均适用于海拔3 000 m以上地区种植。     

马蔺的花部构造及开花授粉生物学特性

对马蔺的花部特征、开花动态、授粉特性及传粉媒介等进行了研究,运用多种处理方式,对马蔺的传粉机制进行测定。结果表明:自然条件下,马蔺5～6月开花,群体花期约40d,盛花期约25d,单花花期约58h。马蔺的繁育系统属于异交,部分自交亲和,自然传粉需要借助传粉媒介,虫媒传粉是其传粉方式之一。     

辽葱5号的选育

辽葱5号是以玉田县五叶齐大葱为母本、朝阳鳞棒葱为父本进行有性杂交，对杂交后代进行季节穿梭选择的方法，即用3年时间进行了3个世代的以株为单位的混合选择及以株系为单位的选择，最后留选1个经济性状整齐、生殖性状稳定的雄性可育母系，经系内混合授粉育成的大葱新品种。该葱生长旺盛、秋葱产量高、葱白较长、叶片直立性好、抗紫斑病能力较强，适合长季节栽培，生育期在120d以上，属中晚熟品种。冬贮干葱可食用率高，适宜北方各省、区种植。     

会宁县太平店镇农村养殖业特点及对策

从行情、技术、信息、资金等方面分析了会宁县太平店镇农村养殖业的现状及特点,并提出了农村养殖业的对策,以期为该镇养殖业的发展提供参考。     

Changes in peroxidase activity and lignin content of cultured tea cells in response to excess manganese

Abstract

The present study was undertaken to identify the effects of manganese (Mn) on the activity of peroxidase (PO) and the amount of ascorbic acid (AsA) and lignin, as well as the cell viability of suspension-cultured tea cells (Camellia sinensis L. cv. Yabukita). Cells were grown in B5 medium (containing 0.06 mmol L^?1 Mn) and cultured for 24 h in medium with a Mn concentration of 0.9 mmol L^?1 as an excess treatment. No significant difference was observed between cellular growth and the viability of the cultured tea cells after treatment with excess Mn compared with cells grown under control conditions, although the content of Mn in cells in the excess Mn treatment was 12-fold higher than that of the control cells. The amount of total AsA was also not affected by the Mn treatment. The activity of ionically wall-bound peroxidase (IPO) increased in the presence of excess Mn, unlike the content of lignin. Conversely, the activities of soluble PO and covalently wall-bound PO decreased with excess Mn. These findings suggested that IPO might contribute to Mn tolerance in tea cells. However, its role in the mechanism(s) of Mn tolerance has not been elucidated.