基于P87LPC764的温室智能温度计的研制

针对一般温室内温度测量电路设计复杂、温度测量与温度控制分离等缺点，以Philips公司生产的P87LPC764为核心器件，以软件方法代替硬件电路，研制了智能化、便携式的数字温度计，实现了对定点温度的采集、处理及控制信号输出。     

基于UEGO的柴油机EGR系统空燃比MAP的制取

在柴油机稳态工况下，通过试验测定了不同空燃比时，NOx、HC、CO、烟度和油耗的变化。通过对测试数据的分析，确定了最佳空燃比MAP。为基于UEGO的柴油机EGR系统的闲环控制奠定了基础。     

杂种大花万寿菊试管苗繁殖

利用组织培养技术繁殖引进的杂种大花万寿菊获得成功,在诱导侧芽时,利用６－卞基腺嘌呤（６－ＢＡ２．５ｍｇ／Ｌ）和玉米素（ＺＴ２．５ｍｇ／Ｌ）ＺＴ对侧芽的诱导效果好于６－ＢＡ,诱导生根的培养基分别为（１）１／２ＭＳ;（２）１／２ＭＳ＋ＮＡＡ０．５ｍｇ／Ｌ;（３）１／２ＭＳ＋ＮＡＡ１ｍｇ／Ｌ;（４）１／２ＭＳ＋ＩＢＡ０．５ｍｇ／Ｌ;（５）１／２ＭＳ＋ＩＢＡ１ｍｇ／Ｌ．ＩＢＡ可以促进万行区形成不定根,质量     

延边黄牛与利延一代牛的短期育肥屠宰试验

经８５ ｄ 的育肥试验结果表明,在育肥期间利延Ｆ１ 牛的体高、体长、胸宽、胸深、胸围和髋宽等体尺的增长值均高于延边黄牛,其中利延Ｆ１ 牛髋宽的增长值显著高于延边黄牛（ｐ ＜ ０ ．０１） ．育肥期间延边黄牛与利延Ｆ１ 牛的日增重分别为０ ．８９ ｋｇ 和１ ．０６ ｋｇ,利延Ｆ１ 牛比延边黄牛高１９ ．１ ％ ,差异显著（ｐ＜ ０ ．０５） ;消化试验结果表明,延边黄牛与利延Ｆ１ 牛对日粮中的粗蛋白、粗脂肪、中性洗涤纤维和酸性洗涤纤维的消化率差异不显著（ｐ ＞ ０ ．０５） ;屠宰试验结果表明,延边黄牛与利延Ｆ１ 牛的屠宰率分别是５１ ．６ ％ 和５４．４ ％ ,净肉率分别是３９ ．２ ％ 和４３ ．９ ％ ,肉骨比分别是３ ．１５ 和４ ．１８ ,眼肌面积分别是７２ ．６ ｃｍ２ 和８８ ．６ ｃｍ２ ．经检验,差异均极显著（ｐ ＜ ０ ．０１） ;对牛肉的一般化学成分分析结果表明,延边黄牛与利延Ｆ１ 牛之间差异不显著（ｐ＞ ０ ．０５） ．     

玉米穗,茎腐病禾谷镰孢菌和串珠镰孢菌同工酶分析

对沈阳、河北、吉林、北京等地区玉米穗、茎腐病禾谷镰孢菌和串珠镰孢菌进行了可溶性蛋白和同工酶聚丙烯酰胺凝胶电泳,以研究玉米穗、茎腐病同种镰孢菌是否存在寄生专化性或生理分化现象。结果表明：各地区的玉米穗、茎腐病串珠镰孢菌同源性高,亲缘关系近,相似性系数在0．86～0．98之间,菌株间差异仅表现为个体间多样性。而两种病害禾谷镰孢菌亲缘关系困地区不同而有差异,河北、吉林两地区穗、茎腐禾谷镰孢菌间均具有很近的亲缘关系,相似性系数在0．93以上;但沈阳地区禾谷镰孢菌亲缘关系相对较远,相似性系数仅为0．65～0．67。     

精养鱼池施用新型磷肥——“鱼特灵”

本文通过在精养鱼池中施用新型磷肥“鱼特灵”,使池水有效磷由10微克/升以下提高到30微克/升以上,有效氮明显下降,有效N/P保持在35～50:1,浮游植物数量显著增加。试验地浮游植物数量(Y)与总铵(NH_4-N+NH_3-N)(X_1)、NO_3-N(X_2)、PO_4-P(X_4)呈如下关系式:Y=55.94-0.67X_1-11.61X_2-47.95X_4(r=0.73,n=28,P<0.05)达到了增施磷肥,促进氮肥利用和浮游植物增殖,改善水质,提高池塘初级生产力的目的。施用“鱼特灵”后,池塘平均净产增长11.6%,其中鲢、鳙净产增长13.7%。     

尾叶桉 湿地松1～6年生施肥效应研究

尾叶桉、湿地松１～６年生幼林施肥结果表明：１．尾叶桉在缺Ｐ的立地上,施Ｐ肥的效应极其显著。施Ｐ各处理在１～６年生时均显著或极显著地大于对照,其中以施ＮＰＫ全肥最好,６年生的树高、胸径和材积与对照比较,分别增长３５％,６９％,２４３％。而施ＮＫ肥的生长与对照差别不大。湿地松１～２年生的幼林施肥效果不大显著,３～６年生时才达显著差异,其中以施ＮＰＫ全肥的效果最好,６年生的树高、胸径和材积与对照比较仍分别增长１３％,１８％,５２％。２．尾叶桉、湿地松施Ｐ肥的各水平均达显著或极显著差异,施Ｎ、Ｋ肥的只在个别年度有差异。３．偏相关和复相关分析结果表明,尾叶桉施肥肥效主次顺序是Ｐ、Ｎ、Ｋ,湿地松是Ｐ、Ｋ、Ｎ。两树种均在５,６年生时,Ｎ、Ｐ、Ｋ的肥效最高。４．最优施肥配方：尾叶桉１～３年生以施Ｎ３Ｐ３为好,５～６年生以施Ｎ３Ｐ３Ｋ２为佳。湿地松１～２年生不用施肥,或薄施Ｎ肥;３～６年生以施Ｎ１Ｐ３Ｋ１较好。     

串番茄新品种红星302 的选育

红星302 是以抗病、耐低温弱光材料01-61 为母本,以01-23 为父本育成的串番茄一代杂种,无限生长类型,果实串收,果色亮红,果实椭圆形,酸甜适口,品质佳,VC 含量为364 mg · kg^-1,可滴定酸0.44%,可溶性糖4.25%,平均单果质量30.79 g,硬度为11.40×10⁵ Pa。单株结果数约为53个,产量4 900 kg ·（ 667 m²）^-1 左右,高抗蕨叶病毒病、青枯病,适宜河北地区冬季日光温室和早春保护地栽培。     

保护地黄瓜新品种唐杂6 号的选育

唐杂6 号是以雌性系S16 为母本,以自交系S26 为父本配制的强雌型黄瓜一代杂种。生长势强,商品瓜短棒状, 瓜长12～14 cm,横径4.0～4.3 cm,非特异性环境下雌花率95% 以上,瓜皮嫩绿有光泽,白刺,刺瘤稀小,平均单瓜质量 131.8 g 左右,高抗霜霉病,抗细菌性角斑病,耐白粉病;春保护地栽培平均产量可达8 000 kg·（667 m²） ^-1,秋冬保护地栽 培产量可达6 500 kg·（667 m²） ^-1;适合河北、北京、天津及东北地区春、秋保护地种植。     

Role of miR-219 and TGFBR2 in pathogenesis of renal fibrosis

AIM:To study the role of microRNA-219 (miR-219) in regulation of transforming growth factor-β receptor type 2 (TGFBR2) in renal fibrosis. METHODS:The renal fibrosis patients (n=70) were selected in this stu-dy, and 20 cases of healthy people were selected as control group. RT-qPCR was used to detect the expression of miR-219 in the serum of the patients with renal fibrosis and control group, and the expression of miR-219 in NRK49F cells after stimulation with angiotensin Ⅱ(AngⅡ) was detected. The protein expression of α-smooth muscle actin (α-SMA) in the NRK49F cells transfected with miR-219 mimics after stimulation with AngⅡ was determined by Western blot. The potential target gene TGFBR2 of miR-219 was screened and verified by the method of luciferase reporter gene. RT-qPCR and Western blot were used to detected the effect of miR-219 mimics on the expression of TGFBR2 at mRNA and protein levels, and the mRNA expression of α-SMA, connective tissue growth factor (CTGF), type I collagen α1 (COL1A1) and COL3A1 in the NRK49F cells was also detected, respectively. The unilateral ureteral occlusion (UUO) mouse model was established and the expression of miR-219 in the renal tissue was monitored. The morphological change of renal fibrosis was observed in the UUO mice after injection of miR-219, and the mRNA expression levels of COL1A1 and COL3A1 were detected. RESULTS:The expression level of miR-219 in the patients with renal fibrosis was significantly lower than that in control group, and the expression of miR-219 in the UUO mice was decreased significantly (P<0.01). The expression level of miR-219 was significantly decreased in the NRK49F cells after AngⅡ stimulation, and miR-219 mimics inhibited the protein expression of α-SMA(P<0.01). miR-219 mimics had a targeted regulatory effect on TGFBR2 gene, which inhibited the mRNA and protein expression of TGFBR2. miR-219 mimics inhibited the mRNA expression of α-SMA, CTGF, COL1A1 and COL3A1. miR-219 also down-regulated the mRNA expression of COL1A1 and COL3A1 in the UUO mice and inhibited the process of renal fibrosis. CONCLUSION:miR-219 inhibits the development of renal fibrosis by inhibiting the expression of TGFBR2, which may become a new target for the diagnosis and treatment of renal fibrosis.