施用含硒有机肥对粉葛产量及硒吸收转运的影响

本研究采用随机区组试验,设置6个处理,研究增施不同量含硒有机肥对粉葛生长、产量及硒在粉葛不同器官中富集状况的变化,探讨增施含硒有机肥对粉葛产量和硒吸收转运的影响.结果表明:随着施肥量的增加,净光合速率先降低后升高,胞间CO2浓度(Ci)、蒸腾速率(Tr)和气孔导度(Gs)则逐渐递增.施用含硒有机肥均显著提高了粉葛块根中...     

不同化学药剂和植物激素处理对珍珠绣线菊种子发芽的影响

用高锰酸钾、硼酸、奈乙酸和吲哚乙酸对珍珠绣线菊种子进行浸种处理,研究不同化学药剂和植物激素对其种子发芽的影响。结果表明:高锰酸钾、硼酸、奈乙酸和吲哚乙酸对珍珠绣线菊种子发芽具有明显的促进作用,它们的最适质量浓度分别为2.0mg.L-1、0.2mg.L-1、20.0mg.L-1和10.0mg.L-1,硼酸和吲哚乙酸的处理效果好于高锰酸钾和奈乙酸。     

Association of Meloidogyne incognita and Fusarium chlamydosporum causing with wilt complex in Northern Karnataka on Coleusforskohlii

A pot culture experiment was carried out to study the interaction of Melodogyne incognita and Fusarium chlamydosporurn on Coleus forskohlii. The nematode multiplication was adversely affected when fungus was inoculated prior to nematode. Simultaneous inoculation of nematode and fungus as well as nematode followed by fungus seven days later, caused 85.5 per cent root rot disease and significant reduction in plant growth as well as nematode multiplication when compared to the inoculation fungus alone or fungus inoculation prior to nematode.     

Screening of genotypes for their resistance againstMeloidogyne incognita and Fusarium chlamydosporum causing wilt complex on Coleusforskohlii Briq.

A pot culture experiment was carried out for the Screening of 11 popular genotypes against to Meloidogyne incognita and Fusarium chlamydosporum wilt complex on Coleus forskohlii. Genotype viz., yellow tubers and Rabakavi local showed resistant reaction, Orange tuber and Nimbanur local showed moderately resistant reaction. Rest of the seven genotvDes showed suscentible reactions.     

彩叶灌木在娄底市园林绿化中的应用与管理

未来的城市对园林绿化中灌木的种植也将趋于向多品种和多色彩化,一些拥有各种色彩叶片的新彩叶灌木品种,将更加受到欢迎。本文结合实践和管理经验,总结了彩叶灌木在娄底市园林绿化中的种类,并提出了彩叶灌木在娄底市的园林应用和发展方向。     

Allelopathic potential of native plants against water hyacinth

Classical biocontrol of water hyacinth using insects in India is constrained by seasonal occurrence of water flow and interrupted host range. Use of fungal pathogens is also difficult due to lack of shelf-life and virulence under severe climatic fluctuations and a lack of knowledge of spray techniques. Hence, the allelopathic potential of native plants is reviewed for use as an alternative bio-control tactic. Dried powder of the leaves of Omavalli Coleus amboinicus L. at 40 g l⁻¹ as a water suspension killed water hyacinth within 24 h reducing the fresh weight by 80.72% and the dry weight by 75.63% within one week. The lowest dose required to kill the whole plant was 10 g l⁻¹. Coleus powder was injurious to cut leaves of water hyacinth even at 0.1 g l⁻¹ dose as it was absorbed directly by the cut leaf, indicating that the dose required to kill the whole plant could still be reduced, if either the natural product or the active principle is formulated for absorption through foliage.     

广藿香~(60)Co-γ诱变体植株遗传多样性的ISSR分析

从广藿香的同一大田植株上取外植体构建了一批无菌材料。将这批无菌材料分别经过0,45,60,75,90 Gy的~(60)Co-γ射线辐照诱变处理,获得了172份Co-γ射线诱变体材料和1份对照无菌材料(CK;0 Gy)。分别提取这173份无菌材料的基因组DNA,用14条ISSR有效引物对173份种质材料的基因组DNA进行PCR扩增,共获得297条扩增产物,其中多态性条带为68条,诱变材料的多态位点比率PPB为43.13%,观测等位基因数Na=1.431 3,有效等位基因数Ne=1.257 3,Nei's基因多样性H=0.146 5,Shannon信息指数I=0.217 5。173份供试材料之间遗传关系系统树由UPGMA法,根据所得遗传相似系数矩阵构建。系统树图表明,当GS(遗传相似系数)值取0.09GS0.42时,可以把供试的173份材料划分为2个类群:即Ⅰ类群和对照(CK);当GS=0.44时,可把Ⅰ类群分为2个亚类群,即Ⅱ类群和90 Gy-43号材料;当GS值取0.46GS0.48时,又可把Ⅱ类群分为2支,即Ⅲ类群和75 Gy-13号材料。虽然有部分不同辐射剂量处理的诱变植株聚类在一起,但是总体上是,相同辐射剂量处理组的诱变植株聚为一类。结果表明,广藿香无菌材料经用~(60)Co-γ射线辐照诱变后发生变异频率较大,为此,~(60)Co-γ射线辐照诱变可作为广藿香种质创新的一种有效手段。     

红腹柄天牛危害与寄主树种的关系

探讨红腹柄天牛危害与罗浮栲、甜槠、细柄阿丁枫3种树种的树皮形态特征、木材含水量、容积重、硬度的关系,结果表明:红腹柄天牛的危害与寄主树皮的粗糙程度有着密切的关系,罗浮栲和甜槠树皮粗糙,适宜于红腹柄天牛成虫产卵,其中罗浮栲有虫株率高达90%,甜槠有虫株率为70%,重度危害;而细柄阿丁枫树皮光滑,有虫株率只有10%,轻度危害。寄主树种的木材基本容积重和含水率均与红腹柄天牛的危害没有直接相关。鲜材、干材硬度高的树种不易受红腹柄天牛的危害,甜槠、罗浮栲材质硬度明显低于细柄阿丁枫,受害较重;同一树种中材质较硬的植株被害程度更轻。     

抗菌肽B、D双基因表达载体的构建及转化广藿香的研究

将人工合成的柞蚕抗菌肽D基因和天蚕抗菌肽B基因分别接上启动子和终止于，克隆到双元表达载体PBin19上，构建成双价抗菌肽基因表达载体pTBD。用三亲交配的方法将pTBD导入根癌农杆菌菌株LBA4404，再通过农杆菌将抗菌肽B、D基因导入广藿香，获27个转基因植株。Southern杂交结果表明，抗菌肽B、D基因已同时整合进3个株系染色体组中。与病原细菌Pseudomonassolanacearum试管共培养结果表明，转基因植株获得较强抗病性。盆栽接菌试验结果也表明．转基因植株具有较强抗病性。     

彩叶草组织培养研究

以唇形科鞘蕊花属彩叶草(Coleus blumei Benth.)叶片为外植体,进行组织快繁的研究.结果表明:叶片组织培养中,最适宜的消毒处理为消毒剂采用0.1%升汞溶液,加入3～4滴吐温80,消毒时间为5 min,总体污染率低于20%;最适的愈伤组织诱导及继代培养基为1/2MS 2.0mg/L 6-BA 1.0 mg/L NAA的组合,其平均诱导率为72%;最适分化培养基是1/2MS 1.0 mg/L 6-BA 0.5 mg/L NAA的组合,其平均分化率为85.4%;彩叶草不定芽的最适生根培养基是1/2MS O.5 mg/L NAA组合,其平均生根率为92.5%.