鸡Hsc70基因5′侧翼区SNP与耐热性状的相关分析

为研究Hsc70基因作为鸡抗病育种中一种分子标记的可能性,本试验利用克隆测序技术对清远麻鸡、广东温氏矮脚黄鸡、灵山鸡和隐性白羽洛克鸡4个品种共20个个体Hsc70基因的5′侧翼区进行多态性筛查,发现10处变异,包括8个SNPs和2个插入/缺失突变。通过分析,选取C.-521A〉C位点,利用PCR-RFLP方法分析了此位点在灵山鸡和隐性白羽洛克鸡群体中的基因型分布,所得分型结果与测定的耐热性状（T3、皮质酮、CD3＋、CD4＋和CD8＋）进行关联分析,结果表明：常温状态下（15℃）,在隐性白羽洛克鸡群体中,位点C.-521A〉C与CD3＋、CD4＋和CD8＋显著相关（P〈0.05）,其中AA基因型个体CD3＋值显著低于AC基因型个体,而CD4＋与CD8＋值显著高于AC基因型个体;热应激状态下（35℃）,在灵山鸡群体中,位点C.-521A〉C与CD8＋极显著相关（P〈0.01）,CC基因型个体CD8＋值极显著高于AA和AC基因型。     

Estimation of litchi (Litchi chinensis Sonn.) leaf nitrogen content at different growth stages using canopy reflectance spectra

The estimation of crop nitrogen status in fresh vegetation leaf using field spectroscopy is challenging due to the weak responses on leaf/canopy reflectance and the overlapping with the absorption features of other compounds. Although the spectral indices were proposed in the literature to predict leaf nitrogen content (LNC), the performance of selected spectral indices to estimate the LNC is often inconsistent. Moreover, the models for nitrogen content estimation changed with the growth stage. The goal of this study was to evaluate the performance of published indices, ratio of data difference index (RDDI) and ratio of data index (RDI) developed by band iterative-optimization algorithm in LNC estimation. The correlation analysis, linear regression and cross validation were used to analyze the relationship between spectral data and LNC and construct the best performed estimation model. The study was conducted by the data of five growing seasons of litchi from the orchards in Guangdong Province of China. Results showed that the relationship between chlorophyll (Chl) related spectral indices and LNC varied with the growth stage. Even in flower bud morphological differentiation stage and autumn shoot maturation stage, there were not significant correlations between the proposed spectral indices and LNC. Besides it is difficult to estimate the LNC by the general model across the growth stages due to the integrated effects of cultivar, biochemical, canopy structure, etc. The band iterative-optimization algorithm can improve the sensitivity of spectral data to LNC to some extent. The optimal RDDI performed better than other indices for the synthetic dataset and the dataset in each growth stage. And the sensitive bands selected in the optimal indices at each growth stage are not consistent, which are not only related to the Chl absorption but also other biochemical components, such as starch, lignin, cellulose, protein, etc. In general, the LNC can be estimated by the optimized CR-based RDDI indices in autumn shoot maturation stage, flower spike stage, fruit maturation stage, and flowering stage with the R² > 0.50 and RMSE < 0.14. Although there were the significant relationship between RDIs and RDDIs in flower bud morphological differentiation stage, the highest R²s of the model developed by RDDIs and RDIs were less than 0.50 in cross validation. This study indicated that the applicability of canopy reflectance to estimate litchi LNC was closely related to the growth stage of litchi. Growth stage-specific models will be preferred for estimating litchi LNC estimation.     

Soybean yield and yield components as influenced by the single and repeated flaming

Field experiments were conducted to study the impact of single and multiple flaming on crop injury, yield components, and yield of soybean. The goal of this experiment was to determine the number of the maximum flaming treatments which soybean could tolerate without any yield loss. The treatments consisted of a non-flamed control, and broadcast flaming conducted one time (at VC-unfolded cotyledon, V2-second trifoliate, and V5-fifth trifoliate), two times (each at VC and V2, VC and V5, and V2 and V5 stages), and three times (at VC, V2, and V5 stages) resulting in a total of eight treatments. All plots were kept weed-free for the entire growing season by hand hoeing. A propane dose of 50 kg ha⁻¹ was applied with torches parallel to the crop row and at an operating speed of 4.8 km h⁻¹ for all treatments. The response of soybean was measured as visual injury ratings (at 7 and 28 days after treatment – DAT) as well as effects on yield components and yield. Broadcast flaming conducted once (at VC or V5 stage), as well as twice (at VC and V5 stages) exhibited the lowest injury of about 8% at 28 DAT. Any treatment that contained flaming at V2 stage resulted in more than 70% injury at 28 DAT. The highest crop yields were obtained from the non-flamed control (3.45 t ha⁻¹) and the plots flamed once at VC (3.35 t ha⁻¹), V5 (3.32 t ha⁻¹), and two times at VC and V5 (3.24 t ha⁻¹), which were all statistically similar. Soybean flamed at V2 stage had lower yields (1.03 t ha⁻¹ at V2, 0.46 t ha⁻¹ at VC and V2, and 0.38 t ha⁻¹ at V2 and V5). The lowest yields were in soybean flamed three times (VC, V2, and V5 stages), which yielded only 0.36 t ha⁻¹. These results indicate that soybean could tolerate a maximum of two flaming treatments at VC and V5 growth stages per season without any yield reduction.     

不同根颈直径苜蓿生理生化特性对低温胁迫的响应

为了探究不同颈粗苜蓿（Medicago sativa L.）的抗寒性与其根颈中生理生化特性的关系。以‘公农1号’紫花苜蓿为试验材料,对不同颈粗（3.50 mm,5.25 mm,7.00 mm）苜蓿根系进行低温胁迫（4℃,-10℃,-15℃,-20℃）处理,测定了苜蓿根颈活力、非结构碳氮含量和抗氧化酶活性。结果表明：低温胁迫对苜蓿根颈活力、非结构碳氮含量、非结构碳氮比（Cnstructured carbon nitrogen ratio,C/N）及过氧化氢酶（Catalase,CAT）活性均有极显著影响（P<0.01）;随低温胁迫的增加不同颈粗的苜蓿根颈活力均降低,可溶性糖和可溶性蛋白含量均呈增加-降低-增加的变化,淀粉含量、C/N和CAT活性均呈先增加后降低的趋势;苜蓿根颈活力大小与根颈中可溶性糖含量、可溶性蛋白含量、CAT活性及C/N显著相关（P<0.05）。研究认为,根颈直径大的苜蓿抗寒性较强,其通过调控根颈中可溶性蛋白、可溶性糖含量和CAT活性及C/N以适应低温胁迫。     

Effect of relationships among clinical mastitis incidence,reproductive performance,and culling rate on the lifetime of dairy cows at Hiroshima University Farm

Farm managers' decision to cull dairy cows is based on the cows' milk production, history of disorder(s), and reproductive performance, each of which affects dairy cows' lifetime (herd life and productive lifespan). We investigated the relationships among the incidence of clinical mastitis (CM), the reproductive performance, and the culling rate. We also assessed the effects of these relationships on the lifetimes of dairy cows, using the records made before and after the introduction of an automatic milking system (AMS) at Hiroshima University Farm. Milk yield, CM incidence density, and culling rate of dairy cows increased after the AMS introduction. The CM incidence was associated with an elongation of the calving interval in cows with the same parity. CM in the 1st parity might have caused the reductions of the cows' lifetime and their parity at culling. A higher age at first calving (AFC) was associated with an increase in culling rate but did not lead to a significant decrease in lifetime. Investigations of the factors mediating CM in the 1st parity or AFC with CM incidence or culling rate in the later stages might contribute to the control of lifetime of dairy cows.     

葫芦砧木低温处理鉴定方法研究

对2个具有低温抗性差异的葫芦砧木A12、A05及西瓜TRM进行低温处理,试验结果表明,葫芦幼苗(0.43~0.71)的低温抗性显著高于西瓜(1.0),4℃条件下A12的耐低温性高于A05,6℃条件下相反;3叶期2个砧木间冷害指数差异显著,且叶绿素含量变化均达极显著,叶绿素含量变化程度与冷害指数具有显著正相关(R2=0.65),因此,叶绿素含量可以作为耐低温葫芦砧木材料选择的一个标准,且3叶1心期6℃为相对较好的低温处理条件。     

20份欧洲型黄瓜种质资源的半致死温度与耐热性研究

为探讨不同欧洲型黄瓜种质资源对高温的适应性差异,为耐热种质资源筛选及耐热机理的深入研究提供理论和实践依据,以20份欧洲型黄瓜的叶片为材料,设置6个高温处理,以室温为对照,测定浸提液电导率值并计算相对电导率,通过拟合Logistic方程,计算半致死温度(LT50)。试验结果表明,随着温度升高,相对电导率呈"S"型曲线,且相对电导率与处理温度存在显著的直线相关关系,通过计算"S"型曲线拐点求得20份欧洲型黄瓜的半致死温度在45~51℃,其中33-2的半致死温度最高,达到50.09℃,37-1的半致死温度最低,为45.82℃。20份欧洲型黄瓜的耐高温能力由强到弱依次为33-275-232-233-111-231-209-236-276-136-137-210-135-276-211-143-235-175-132-137-1。因此,半致死温度可作为评价欧洲型黄瓜耐热性的一个可靠指标。     

不同基因型大麦苗期对干旱胁迫的响应及HvPIPs基因表达特征分析

为研究不同基因型大麦苗期对干旱胁迫的响应,挖掘优质抗旱的大麦种质资源,以4份不同基因型大麦(2个高抗品种ZDM5430和ZDM5458,2个干旱敏感型品种7DCADA和IL-12)为材料,利用聚乙二醇(PEG-6000)模拟干旱条件,综合分析不同品种大麦的苗期抗旱性差异;并通过对不同品种根系进行PIPs实时定量分析,研究水通道蛋白AQPs基因与干旱胁迫之间的应答关系。结果表明,持续干旱胁迫会使大麦生长变缓,各处理苗长、根长、叶含水量和根含水量都呈下降趋势,其中,4个品种的大麦叶含水量在干旱胁迫14 d时下降最为显著,与对照相比,其降幅分别为29.14%、52.64%、21.67%和72.15%。干旱胁迫对大麦苗期干物质积累量和根冠比的影响较小,各处理间差异不显著;但持续干旱后,抗旱品种ZDM5430的根冠比呈上升趋势,在胁迫7、14、21 d时较CK分别增加了4.92%、42.86%和21.05%。随着胁迫时间的延长,大麦旗叶叶绿素含量和根系活力也呈下降趋势,其中ZDM5430和ZDM5458较对照组降幅较小,说明抗旱品种的形态指标、叶绿素含量及根系活力受干旱胁迫的影响较小。通过PIP...     

Changes in the herbicide sensitivity and competitive ability of Abutilon theophrasti over 28 years: Implications for hormesis and weed evolution

BACKGROUND

The potential of weed species to respond to selection forces affecting the evolution of weedy traits such as competitive ability is poorly understood. This research characterized evolutionary growth changes in a single Abutilon theophrasti Medik. population comparing multiple generations collected from 1988 to 2016. A competition study was performed to understand changes in competitive ability, and a herbicide dose–response study was carried out to assess changes in sensitivity to acetolactate synthase-inhibiting herbicides and glyphosate over time.

RESULTS

When grown in monoculture, A. theophrasti biomass production per plant increased steadily across year-lines while leaf number decreased. In replacement experiments, A. theophrasti plants from newer year-lines were more competitive and produced more biomass and leaf area than the oldest year-line. No clear differences in sensitivity to imazamox were observed among year-lines. However, starting in 1995, this A. theophrasti population exhibited a progressive increase in growth in response to a sublethal dose of glyphosate (52 g a.e. ha⁻¹), with the 2009 and 2016 year-lines having more than 50% higher biomass than the nontreated control.

CONCLUSION

This study demonstrates that weeds can rapidly evolve increased competitive ability. Furthermore, the results indicate the possibility of changes in glyphosate hormesis over time. These results highlight the importance of the role that rapid (i.e., subdecadal) evolution of growth traits might have on the sustainability of weed management strategies. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.     

青海东部农区引进燕麦品种各器官C、N、P生态化学计量学特征变化研究

以4个引种燕麦(Avena sativa)为典型代表,对其不同生育时期根、茎、叶中的C、N、P含量及其化学计量学特征进行测定,探讨不同生育时期燕麦C、N、P元素含量及其化学计量比的变化规律,为燕麦饲草的科学收获提供理论依据。结果表明：燕麦全株C、N、P含量分别为322.30～333.97、17.42～75.62、2.74～5.42 mg·g^-1,燕麦根C、N、P含量分别为298.42～317.92、11.47～73.71、2.82～3.42 mg·g^-1,燕麦茎C、N、P含量分别为311.25～338.86、10.15～75.16、2.44～5.06 mg·g^-1,燕麦叶C、N、P含量分别为330.80～372.47、30.64～113.80、2.59～8.65 mg·g^-1;各器官间C、N、P含量表现为叶>茎>根。此外,燕麦各器官C、N、P含量的积累过程具有一定季节特征,C含量积累过程受生育时期影响较小,表现出较强的稳定性;而N和P含量的积累过程受生育时期影响较大,其在拔节期～抽穗期均...