响应曲面法优化高粱挤压最佳操作参数的研究

以高粱为原料,研究系统操作参数,包括:物料含水率、喂料速度、螺杆转速、机筒温度对目标参数的影响,并以响应曲面法优化最佳操作参数,优化得到的最佳操作参数为:物料含水率15.77%,喂料速度26.97r/min,螺杆转速181.20r/min,五区温度158.28℃。     

1株解淀粉芽孢杆菌B7快速液态发酵优化工艺研究

本试验以1株饲用益生菌解淀粉芽孢杆菌B7（Bacillus amyloliquefaciens B7，B.amyloliquefaciens B7）为研究对象，为了增强其在液态条件下的发酵水平，提高其芽孢发酵工艺优化效率，拟采用测定芽孢吡啶二羧酸（dipicolonic acid，DPA）浓度的方法来代替传统的平板芽孢计数法，以探讨此测定方法在芽孢发酵优化工艺中的可行性。首先，通过单因素试验筛选出对菌株产孢有显著促进作用的因子，进一步通过正交优化试验确定蛋白胨、玉米粉、大米蛋白粉和Mn²⁺组合的最优水平。结果表明，发酵液中的芽孢浓度与测定的DPA荧光强度呈线性相关，在蛋白胨20 g/L、玉米粉10 g/L、大米蛋白粉20 g/L、Mn²⁺1.0 mmol/L时发酵48 h，检测到DPA荧光强度达到最大，其相应的芽孢浓度达到7.0×10⁹ CFU/mL。与实验室常用的LB培养基相比，芽孢浓度提高了3.3倍；与工业生产用培养基相比，芽孢浓度提高了2.2倍。本研究为芽孢杆菌发酵过程中芽孢浓度测定提供了一种快速方法，为工业菌株B.amyloliquefaciens B7的发酵提供了一种成分简单且高产芽孢的培养基。     

苦瓜对白粉病的抗性与相关生理生化指标的关系

为探讨苦瓜抗白粉病的生理生化机制,以对白粉病不同抗性的4个苦瓜品系为材料,研究苗期感染白粉病菌后,苦瓜叶片生理生化指标的变化。结果表明:接种后,叶绿素和可溶性蛋白质量分数均呈先上升后降低的趋势;可溶性糖质量分数呈下降-上升-下降-上升的趋势;抗病品系的可溶性糖、叶绿素质量分数和过氧化物酶(POD)、多酚氧化酶(PPO)活性均高于或显著高于感病品系和高感品系;抗病品系的抗坏血酸(AsA)质量分数的上升和下降的幅度均小于感病品系和高感品系;接种后10~20d,抗坏血酸过氧化物酶(APX)活性表现为抗病品系感病品系高感品系。叶绿素、可溶性糖质量分数和POD、PPO活性与病情指数呈显著或极显著负相关。综上说明,白粉病菌侵染苦瓜后,抗病品系可通过保持较高的叶绿素质量分数,增加可溶性糖、可溶性蛋白、AsA质量分数及增强POD、PPO、APX活性来提高抗病性。叶绿素、可溶性糖质量分数和POD、PPO活性均可作为苦瓜对白粉病抗性早期鉴定的指标。     

自然低温胁迫下3种桉树的叶绿素荧光特性研究

对临安市3种桉树幼苗叶片叶绿素荧光特性进行研究,结果表明,自然低温下,各桉树表现出不同的适应性,总的来说,邓桉>柳桉>巨桉;自然低温(日最低气温-3.6℃)对柳桉和巨桉造成较大损害,而邓桉可通过维持相对较高的光化学效率,以及利用不断增加的qN来进行耗散,具有较强的抵抗低温胁迫伤害的能力。     

微喷灌溉在山区农业园区的应用研究

对农业休闲园区内进行微喷灌溉设计、施工和应用效果检验,介绍了山区丘陵地形应用微喷高效节水灌溉的设计参数、施工措施和应注意的问题,为发展山区微喷灌溉、服务农业园区提供参考。     

Design and performance of recirculating aquaculture system for marine finfish broodstock development

Tropical and subtropical climatic conditions in India present an ideal and unique opportunity for being the leader in tropical marine finfish aquaculture. However, the problem persist due to non-availability of marine finfish seed for the culture. In response to this problem, broodstock development of different tropical marine finfishes for seed production was started. The present study was undertaken to design a recirculating aquaculture system (RAS) and studying their performance in managing the various water quality required for the marine finfish broodstock development and breeding. The design of RAS, developed in the present study, included a broodstock tank, egg collection chamber, electrical pump, rapid sand filter, venturi type protein skimmer and biological filter. Two RAS were designed, one was stocked with a demersal fish species, orange spotted grouper (Epinephelus coioides) and the other was stocked with a pelagic fish species, Indian pompano (Trachinotus mookalee) at the rate 1 and 0.5 kg/m³ with a sex ratio of 1:1 and 1:2 (female: male) respectively. Various physio-chemical parameters, viz, total ammonia nitrogen (TAN), nitrite, nitrate, pH, alkalinity, temperature, free carbon dioxide (CO₂) and dissolved oxygen (DO) of both tank water were analyzed to assess the performance of recirculating aquaculture system in maintaining the water quality. Gonadal development of the fishes was assessed and the spawning performance was recorded and finally, economic performance of the system was also evaluated. During the entire experimental period, mean monthly total ammonia nitrogen was less than 0.07 and 0.06 mg L⁻¹ and mean monthly nitrite was less than 0.02 and 0.01 mg L⁻¹ in orange spotted grouper and Indian pompano RAS tanks respectively. The pH (7.8–8.2), DO (>4 mg/L) and alkalinity (100–120 mg/L) were found to be in optimum range in both recirculating aquaculture systems. Carbon dioxide was found to be nil during the entire experimental period in both the systems. In fact these levels were comparable or less than that is reported as the permissible limits for broodstock development. Indian pompano and Orange spotted grouper matured and spawning was obtained with production of fertilized eggs round the year. Economic evaluation showed the price of 10,000 fertilized eggs of orange spotted grouper to be US $ 1.33. The design of RAS devised in the present study is efficient in controlling and maintaining optimum water quality for broodstock development of both demersal and pelagic finfishes. The fishes stocked in RAS attained final maturation and round the year spawning was obtained.     

Soil physical changes and maize growth in a structurally fragile tropical soil due to mulching and duration between irrigation intervals

Under tropical meteorological conditions, the volume of soil explored by plant roots is crucial for crop growth as it allows increased water and nutrient use efficiency. We hypothesized that, under different irrigation intervals, leguminous mulch can extend the duration between irrigation events but maintain crop performance, because decreased evaporative fluxes also reduce constraints to root exploration imposed by mechanical stress. We evaluated the combined effects of leguminous mulch and irrigation intervals on soil physical properties to determine whether the growth and productivity of maize were modified in a structurally fragile tropical soil. The experiment involved the following treatments: 4‐day irrigation intervals with soil mulched (4C) or bare (4S), 6‐day irrigation intervals with soil mulched (6C) or bare (6S), 8‐day irrigation intervals with soil mulched (8C) or bare (8S) and 10‐day irrigation intervals with soil mulched (10C) or bare (10S). Mulch decreased soil penetration resistance and increased to 4 days the favourable time for root development in drying soil. Relative to bare soil, mulch with a 6‐day irrigation interval almost doubled nitrogen uptake post‐tasselling, which decreased nitrogen remobilization and increased the crop growth rate during this stage. These conditions had a positive effect on the transpiration rate and stomatal conductance as well as on the growth and yield of maize. A 6‐day irrigation interval with mulch compared to 4 days with bare soil resulted in similar conditions for root development, but greater uptake of nitrogen (102.73–78.70 kg/ha) and better yield (6.2–5.3 t/ha), which means greater efficiency in nitrogen and water use.     

苹小卷叶蛾在6个苹果品种上的发育与繁殖

为探索不同品种苹果对苹小卷叶蛾生长发育和繁殖的影响,在温度(26±1)℃、相对湿度(80±5)%、光周期L∶D=16 h∶8 h的室内条件下,测定了苹小卷叶蛾在小国光、大国光、红玉、祝光、红星和金帅上的发育历期、存活率和产卵量,并组建了其各自的生命周期表。结果显示,苹小卷叶蛾在6个品种上的发育历期差异显著,全世代发育历期以小国光最短(33.95 d),以红玉最长(39.76 d);平均单雌产卵量以红玉最高(107.24粒),小国光次之(85.00粒);而存活率则无显著差异。生命表参数中净生殖率(R0)、内禀增长率(rm)和周限增长率(λ)均以小国光最高,世代平均周期(T)和种群加倍时间(Dt)以小国光最短,表明6种试验品种中小国光是苹小卷叶蛾最适宜的寄主品种。     

Effect of dietary Moringa stem meal level on growth performance,slaughter performance and serum biochemical parameters in geese

Moringa stem meal (MSM) with a high level of crude fibre (CF) might be developed and utilized in herbivorous geese as an unconventional feedstuff. The aim of this study was to investigate the effect of the MSM level in the diet on the growth performance, slaughter performance, breast meat quality and serum biochemical parameters in geese from 22 to 70 days of age. A one-factor completely randomized design was adopted in our study. A total of one thousand eight 21-day-old geese were randomly divided into six groups, with six replicates per group and 28 birds per replicate. The geese were fed diets containing MSM levels of 0, 20, 40, 60, 80 or 100 g/kg during day 22–70. The dietary MSM level had no effect (p > .05) on the final body weight (BW), average daily gain (ADG) or average daily feed intake (ADFI). The feed/gain ratio (F/G) increased linearly (p < .001) as the dietary MSM level increased. No differences (p > .05) were observed in the slaughter performance, meat quality and the relative organ weight (except for thymus) of the geese (p > .05). The relative weight of the thymus in the geese fed diets with supplementation of MSM was higher than that in the non-supplemented MSM control group (p < .05). In addition, 100 g MSM/kg of diet decreased the serum glucose (GLU) level (p < .05) and increased the alanine transaminase (ALT) enzyme activity (p = .03). Dietary MSM levels of no more than 60 g/kg had no effects on the growth performance and slaughter performance, whereas diets with 100 g MSM/kg increased the F/G and serum ALT enzyme activity, as well as decreasing the serum GLU level. Therefore, MSM provided at a reasonable level could be developed as an unconventional feedstuff for geese at the finisher period.     

提高小麦单产的田间排水暗管规格模拟

改进内蒙古河套灌区田间排水系统的规格对灌区提升作物产量和保障粮食生产有重要现实意义,因此,在河套灌区对所构建的分布式SWAP-WOFOST模型进行了参数率定和验证,然后,对2000—2010年灌区春小麦种植条件下适宜的田间排水暗管规格进行探讨,并模拟评价了该条件下作物产量和水分生产力(WP)的时空分布特征。结果表明,所构建的分布式模型在河套灌区具有较高的适应性。春小麦种植条件下,灌区大部分耕地面积推荐采用田间排水暗管的规格为间距100.0m或75.0m,埋深为2.5m。相比现有排水系统,采用推荐的排水暗管规格,春小麦多年平均作物产量提高了18.5%,多年平均WP提高5.2%。研究结果为进一步改进灌区田间排水系统的效果提供了一定的参考。