《青海草业》1997～2002年发文作者及引文的统计分析

统计了《青海草业》6年载文的作者及引文情况,就本刊发文量、合作度、多产单位、活跃作者群、篇均引文量、引文语种等方面的指标进行了分析,对我省草业工作者的文献需求规律与特点进行了探讨。     

Selection of efficient arbuscular mycorrhizal fungi in the rhizosphere of cashew and their application in the cashew nursery

Mycorrhizal association in cashew (Anacardium occidentale L.) grown in five different plantation areas of South India was investigated. The soil samples were collected from all locations. Physico-chemical properties of rhizosphere soils, arbuscular mycorrhizal fungal spore count and root colonization were recorded. Species isolated were Acaulospora, Gigaspora, Glomus and Scutellospora. The mycorrhizal spore density was maximum at Thanjavur and minimum at Kudumiyamalai. Root colonization percentage oscillated between 20 and 30%. The species of Glomus, G. aggregatum Schenck and Smith, G. fasciculatum (Thaxter) Gerdemann and Trappe and G. mosseae (Nicol. and Gerd.) Gerdemann and Trappe were the most abundant in the majority of the experimental sites. Hence these three species were selected and used for artificial inoculation studies. Of the three mycorrhizal fungi G. fasciculatum significantly increased shoot length, internode number, internode length, number of leaves, stem diameter, root length and root number.     

Effect of various polyamines on production of protocorm-like bodies in orchid—Dendrobium ‘Sonia’

Polyamines, putrescine, spermidine and spermine all at three concentrations viz., 0.2, 0.4 and 1.0 mM were tested, by supplementing to MS basal+4.44 μm BAP medium. The explant used was fractionated plb. Ethylene and methane was measured at 20 and 40 days after inoculation (DAI). Among various polyamines tested, maximum number of plb’s (protocorm-like bodies) were produced in putrescine 0.4 mM treatment. Increase (1.0 mM) or decrease (0.2 mM) in concentration caused a decrease in the production of plb’s. All spermidine and spermine treatments resulted in the production of less number of plb’s than control. No ethylene evolution was observed in any of the polyamine treatments. However, methane evolution was observed in all the polyamine treatments. The absolute amounts, of methane evolved could not be related to the observed plb’s production response. However, when the evolution of methane was more than 1 nmol per gram FW h⁻¹, poor plb’s production was observed.     

Agrobacterium tumefaciens mediated genetic transformation and regeneration of Morus alba L.

An efficient method was established for genetic transformation of Morus alba clone M5 using Agrobacterium tumefaciens mediated gene transfer. Cotyledon explants from in vitro grown seedlings were co-cultivated with disarmed strain LBA 4404 harbouring the binary vector p^BI121 carrying chimeric β-glucuronidase (GUS) and neomycin phosphotransferase (npt II) genes. Maximum transformation frequency of 18.60% was recorded with 48 h of pre-conditioning followed by co-cultivation for the same duration. Expression and presence of transgene was confirmed by histochemical test and polymerase chain reaction. The transgenic plants were micropropagated and successfully acclimatised.     

The effect of combined Azospirillum inoculation and nitrogen fertilizer on plant growth promotion and yield response of the blanket flower Gaillardia pulchella

The investigation was carried out to examine the performance of Azospirillum isolates on growth and N uptake of Gaillardia pulchella with two nitrogen levels. Azospirillum strain OAD-2 inoculation significantly increased plant height, number of leaves per plant, branches per plant and total dry mass accumulation in G. pulchella than other inoculations and/or uninoculated control. The application of 150 kg N ha⁻¹ further enhanced the above plant growth parameters significantly over application of 112 kg N ha⁻¹. The N uptake of G. pulchella was enhanced due to Azospirillum strain OAD-2 inoculation, which was further augmented in the presence of nitrogen. The combination of Azospirillum strain OAD-2 and 150 kg N ha⁻¹ showed the highest N uptake at 120 DAT. Plant growth and yield parameters also increased when inoculated with the non-diazotrophic, but efficient IAA producing, Azospirillum strain OAD-57. From this investigation, it can be concluded that Azospirillum strains OAD-2 and OAD-11 can play an important role in the N nutrition of G. pulchella.     

Suitability of wood fiber substrate for production of vegetable transplants: I. Physical properties of wood fiber substrates

Several experiments were conducted with the objective to find suitable alternatives for peat substrates. Physical properties of wood fiber substrates in comparison to other organic substrates were examined. Volume weight, total pore space, pore size distribution, available water, container and air capacity, saturated hydraulic conductivity and volume loss of these substrates were determined. Wood fiber substrates showed a similar volume weight and total pore space as peat substrates, but lower water retention. Particularly the drainage performance of wood substrates results in a quick drainage of surplus water. An increase of volume weight caused a decrease of total pore space, air content and saturated hydraulic conductivity and an increase in water retention. Evaluation of these parameters under container conditions is critical since they are directly related to irrigation and therefore to plant growth. A compression of wood fiber substrates in the pots is recommended to minimize substrate loss. An increase of compression levels reduced the volume loss to almost 0 vol.%. The effect of wood fiber substrates and their volume weight on plant growth will be reported in a second paper.     

Role of bioinoculants in development of salt-resistant saplings of Morus alba (var. sujanpuri) in vivo

Pot experiments were conducted for the development of salt-resistant saplings of Morus alba (var. sujanpuri) involving bioinoculants, namely arbuscular mycorrhizal (AM) fungi, Azotobacter and indole butyric acid (IBA). The IBA and sodium chloride (NaCl) concentrations were optimized prior to the experiments. By using both low and high concentrations, 15 ppm IBA and 0.05% NaCl (w/v) were found to be optimum in acting synergistically with AM fungi and Azotobacter and also for increasing all the growth parameters and microbial count in the rhizosphere. For in vivo development of salt-resistant saplings, the optimal concentration of IBA, along with AM fungi and Azotobacter in different combinations, was applied. The saplings were irrigated regularly with 0.05% NaCl water. Although growth parameters such as AM infection percentage, AM spores per 100 g soil and Azotobacter cells/g soil were affected by NaCl watering, the inoculation of both bioinoculants significantly enhanced survival percentage of saplings from 25 to 50% under salt stress. Maximum survival (55%) of saplings was found with IBA (15 ppm)+AM fungi+Azotobacter.     

Characterization of soil physical properties and organic matter under long-term primary tillage in a humid climate

Chisel ploughing is considered to be a potential conservation tillage method to replace mouldboard ploughing for annual crops in the cool-humid climate of eastern Canada. To assess possible changes in some soil physical and biological properties due to differences in annual primary tillage, a study was conducted for 9 years in Prince Edward Island on a Tignish loam, a well-drained Podzoluvisol, to characterize several mouldboard and chisel ploughing systems (at 25 cm), under conditions of similar crop productivity. The influence of primary tillage on the degree of soil loosening, soil permeability, and both organic matter distribution throughout the soil profile and organic matter content in soil particle size fractions was determined. At the time of tillage, chisel ploughing provided a coarser soil macrostructure than mouldboard ploughing. Mouldboard ploughing increased soil loosening at the lower depth of the tillage zone compared to chisel ploughing. These transient differences between primary tillage treatments had little effect on overall soil profile permeability and hydraulic properties of the tilled/non-tilled interface at the 15–30 cm soil depth. Although soil microbial biomass, on a volume basis, was increased by 30% at the 0–10 cm soil depth under chisel ploughing, no differences were evident between tillage systems over the total tillage depth. Mouldboard ploughing increased total orgainc carbon by 43% at the 20–30 cm soil depth, and the carbon and nitrogen in the organic matter fraction ≤ 53 μm by 18–44% at the 10–30 cm soil depth, compared to chisel ploughing.     

Rotation and tillage effects on available soil water for winter wheat in a semi-arid environment

The recent adoption of conservation farming systems in the semi-arid Canadian prairies opens up the possibility of replacing the traditional fallow period with non-cereal crops (oilseeds, legumes). However, information on changes to soil water regimes by inclusion of these crops, especially in combination with zero tillage, is sparse. A study was initiated in 1984 on a sandy clay loam soil at Lethbridge, Alberta, to investigate the performance of winter wheat (Triticum aestivum L.) under conventional, minimum and zero tillage in monoculture and in 2-year rotations with fallow, canola (Brassica campestris L.) or lentils (Lens culinaris Medic.)/flax (Linum usitatissimum L.). Conventional tillage in the Lethbridge region is shallow cultivation (10 cm) with a wide-blade (sweep) cultivator. Continuous cropping greatly depleted soil water reserves, resulting in some crop failures. Averaged over 10 years, available water for establishment of winter wheat in fall was least after canola (45 mm), followed by continuous winter wheat (59 mm), lentils/flax (74 mm) and fallow (137 mm). In this semi-arid region, the effect of rotation on soil water was much greater than that of tillage. Zero tillage had relatively little impact on available water to 1.5 m depth. However, once the experiment had been established for 6–7 years, available water in the 0–15 cm depth under winter wheat in spring was greatest under zero tillage. Precipitation storage efficiency during the fallow year was generally unaffected by tillage system.