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21.
Plague, caused by Yersinia pestis, is a flea-borne disease that is endemic in areas throughout the world due to its successful maintenance in a sylvatic cycle, mainly in areas with temperate climates. Burrowing rodents are thought to play a key role in the enzootic maintenance as well as epizootic outbreaks of plague. In the United States, prairie dogs (Cynomys), rodents (Muridae), and ground squirrels (Spermophilus) are susceptible to infection and are parasitized by fleas that transmit plague. In particular, prairie dogs can experience outbreaks that rapidly spread, which can lead to extirpation of colonies. A number of ecological parameters, including climate, are associated with these epizootics. In this study, we asked whether soil parameters, primarily moisture and temperature, are associated with outbreaks of plague in black-tailed prairie dogs and Gunnison's prairie dogs in the Western United States, and at what depth these associations were apparent. We collected publicly available county-level information on the occurrence of population declines or colony extirpation, while historical soil data was collected from SCAN and USCRN stations in counties and states where prairie dogs have been located. The analysis suggests that soil moisture at lower depths correlates with colony die-offs, in addition to temperature near the surface, with key differences within the landscape ecology that impact the occurrence of plague. Overall, the model suggests that the burrow environment may play a significant role in the epizootic spread of disease amongst black-tailed and Gunnison's prairie dogs. 相似文献
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Barnaby Kelly Douglas Thamm Rhonda J. Rosengren 《Veterinary and comparative oncology》2023,21(4):595-604
Canine osteosarcoma is an aggressive cancer, comprising 85% of canine bone neoplasms. Current treatment practices of surgery and chemotherapy increase 1-year survival by only 45%. The curcumin analogue RL71, has demonstrated potent in vitro and in vivo efficacy in several models of human breast cancer through increased apoptosis and cell cycle arrest. Thus, the present study aimed to investigate efficacy of curcumin analogues in two canine osteosarcoma cell lines. Osteosarcoma cell viability was assessed using the sulforhodamine B assay and mechanisms of action were determined by analysing the levels of cell cycle and apoptotic regulatory proteins via Western blotting. Further evidence was obtained using flow cytometry to detect cell cycle distribution and the number of apoptotic cells. RL71 was the most potent curcumin analogue with EC50 values of 0.64 ± 0.04 and 0.38 ± 0.009 μM (n = 3) in D-17 (commercial) and Gracie canine osteosarcoma cells, respectively. RL71 significantly increased the ratio of cleaved-caspase 3 to pro-caspase 3 and the level of apoptotic cells at the 2× and 5× EC50 concentration (p < 0.001, n = 3). Furthermore, at the same concentration, RL71 significantly increased the number of cells in the G2/M phase. In conclusion, RL71 has potent cytotoxic activity in canine osteosarcoma cells triggering G2/M arrest and apoptosis at concentrations achievable in vivo. Future research should further investigate molecular mechanisms for these changes in other canine osteosarcoma cell lines prior to in vivo investigation. 相似文献
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为探究胚根线粒体抗坏血酸-谷胱甘肽(AsA-GSH)循环响应低温胁迫的抗氧化作用机制,以紫花苜蓿种子为材料,研究了不同温度(10和20℃)处理下发芽特性、不同吸胀时间(6、12和24 h)胚根线粒体AsA-GSH循环酶活性、抗氧化物以及过氧化氢(H2O2)含量的变化规律。结果表明,吸胀12和24 h后,10℃处理的胚根线粒体H2O2含量高于20℃。吸胀24 h期间,10℃处理的胚根线粒体谷胱甘肽还原酶(GR)活性均低于20℃。吸胀12和24 h后,10℃处理的胚根线粒体抗坏血酸(AsA)含量均低于20℃。10℃条件下吸胀24 h后,与20℃处理相比,胚根线粒体谷胱甘肽(GSH)含量显著降低(P<0.05)。苜蓿种子在10℃条件下吸胀萌发时,主要通过降低AsA-GSH循环中GR、单脱氢抗坏血酸还原酶(MDHAR)、过氧化物酶(POD)活性和AsA、GSH含量,使胚根线粒体内的H2O2积累,产生氧化损伤,继而影响种子萌发的正常进程。 相似文献
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Peng Donglin Dai Zhengyuan Zheng Lianqin 《保鲜与加工》1997,(1):104-108
The characteristics of pretreatment and cyclic control stream are analysed:the form of cyclic embedded hardware circuit,the idea for designing the pretreatment program.The chart model of the system is established preliminarily. 相似文献
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In order to improve waveform performance of light HVDC system, a lot of switches which operate in high frequency are used, which causes large switching losses and results high cost. A new Buck type voltage source converter(BVSC) is proposed. The new BVSC comprises a dual-buck converter and a three-phase inverter operating in grid frequency. Only two high frequency switches are used, which results good output waveform performance. In order to reduce the high frequency switching losses, a novel improved double-frequency Buck type voltage source converter(DBVSC) is also proposed. An additional dual-Buck converter is added to the BVSC, the added dual-Buck converter deals with the fundamental power that operates in low frequency, and BVSC only deals with harmonic power that operates in high frequency. The output waveform is enhanced and the switching losses are reduced in DBVSC, and the power rating is also increased. The DBVSC is very suitable for large power rating application. The one cycle control theory is adopted to control the proposed DBVSC with analog circuit, which is very simple. The proposed DBVSC and control method are verified by simulation. 相似文献
28.
Development Management (DM) in decision phase, Project Management on behalf of the owner (OPM) in implementation phase and Facility Management (FM) in operation phase are three isolated management tools during traditional life cycle management of construction project. As a result, a lot of potential disadvantages are aroused. In recent years, many research organizations try to reform the traditional method using different integration means. This paper tries to explore into integration of DM, OPM and FM to overcome the malpractice of traditional life cycle management of construction project. The research focuses on three aspects: 1.putting forward the concept of Life Cycle Integrated Management (LCIM), based on management integration theory; 2. Raising a new idea to owner's (operator's) management organization design, which emphasizes on the balance of life cycle objectives; 3. Analyzing an information module, level and method of system integration of Life Cycle Integrated Management Information System. 相似文献
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针对车辆真实工况与标准工况实时偏差导致的整车能量管理性能下降问题,提出一种基于工况偏差在线计算的插电式混合动力汽车(PHEV)能量补偿控制方法。根据车辆工况识别结果建立实际工况与标准工况之间偏差的数学表达式;以油电耗比为指标函数,构建能量管理系统控制偏差数学模型,给出工况偏差与控制偏差函数关系的离线建模方法;在此基础上,以工况偏差为输入,以控制偏差为被控对象,设计能量补偿模糊控制器,给出控制偏差的模糊控制规则和动力补偿分配权值。基于某控制策略对本文所提出的方法进行仿真验证,结果表明:所提出的能量分配补偿控制方法能够进一步降低整车燃油消耗,进而提升PHEV能量管理性能。 相似文献