酸角挥发油香味成分分析及在卷烟中的应用

利用超临界CO2流体萃取酸角挥发油，平均出油率为2．46％。并利用气相色谱仪对酸角挥发油的20种主要香味成分进行了定量分析。通过卷烟加香试验发现，酸角挥发油能有效地掩盖卷烟杂气，使烟气柔和，香气细腻，对改进烟气质量作用明显。     

家蚕品种最适环境片区的划分

对家蚕品种最适环境片区划分进行了研究，初步得到如下结果：参试的8个地区可以划分为两个环境片区。第一个环境片区包括四川、重庆和浙江；第二个环境片区包括陕西、山东、安徽、湖北和镇江；第一个环境片区的最适基因型是品种B；第二个环境片区的最适基因型是品种C。如果在各环境片区推广相应最适基因型比在所有地区只推广品种C，则全区平均万蚕收茧量可提高2．1％。     

Preparation of Cathepsin L1 Monoclonal Antibody Against Fasclala gigantica and Establishment of Double Antibody Sandwich ELISA

【Objective】 This study was intend to obtain cathepsin L1(rFgCat L1) specific monoclonal antibody and construct the double antibody sandwich ELISA.【Method】 Five BALB/c mice were immunized with 1 mg/mL rFgCat L1 protein for four times.Mouse splenocytes were isolated and fused with SP2/0 cells to construct hybridoma cells.Strong positive hybridoma cell lines were screened, 1×10⁶ cells were injected intraperitoneally per mouse to prepare monoclonal antibodies.Antibody titer and antigenic epitope were detected using ELISA method, antibody subtype and specificity were identified using Western blotting method.The double antibody sandwich ELISA was constructed by combining the anti-rFgCat L1 polyclonal antibody, and its sensitivity and specificity were tested.The positive and negative critical value was screened by 20 negative sera with positive control, and the constructed double antibody sandwich ELISA was verified by 47 goat positive sera and 47 dairy cow positive sera.【Result】 After immunization, the antibody titers in serum of 4 mice were all more than 10⁴.After isolated mouse with the highest immune response spleen cells were fused with SP2/0 cells total of 8 of them were positive cell lines were obtained after selective culture.5D5 and 7G6 were identified as strong positive strains with stable antibody secretion.After multiple subcloning screens and subcultures, the antibodies secreted in the cell supernatant were stable, with titers of 2⁹ and 2¹⁰ respectively, with ascites titers of 10⁷ and 10⁸.Western blotting and antibody subtype identification kits identified that the two antibodies were IgG1 type and the light chain was kappa type, both of which could specifically bind FgESP.According to the same antigen site was recognized by the two kinds of antibodies, the antigen titer of the two monoclonal antibodies were comparied, 7G6 was used as the coating antibody, and anti-rFgCat L1 was used as the enzyme-labeled secondary antibody.The optimized condition of method was that 7G6 was coated at a concentration of 2 μg/mL, the dilution concentration of anti-rFgCat L1 polyclonal antibody was 25 μg/mL, the dilution of Don-HRP-conjugated was 1∶4 000, 5% skimmed milk powder was selected as the blocking solution and the color development time was 25 min.The method was proved that could recognize the lowest antigen concentration of 0.625 μg/mL, also could specifically recognize antigen of Fasciola fasciatus.The constructed sandwich ELISA method was used for antigen detection of 47 dairy cow positive serum and 47 goat positive serum infective samples kept in the laboratory and the positive antigen rate were 72.3% and 78.7%, respectively.【Conclusion】 Anti-rFgCat L1 monoclonal antibody was successfully prepared and the double-sheet sandwich ELISA method for fascioliasis was constructed, which provided a good theoretical basis and material basis for the development of low-cost and rapid diagnostic kits.     

GGR6号植物生长调节剂对华北落叶松造林的影响及作用机理

造林前用双吉尔—GGR6号植物生长调节剂对华北落叶松苗进行浸根处理，可提高造林成活率和林木生长量。其作用机理在于调节了苗木内源激素的含量，提高了苗木体内酶的活性，增强了苗木细胞新陈代谢的能力。     

中国草芦对土壤营养吸收的效率(英文)

中国草芦(Phalaris arunainacea L.)是禾本科草芦属多年生根茎型优良禾草。对其进行N、P、K、NP、NK、PK、NPK、CK八个处理,在分蘖期采用M.K.Ka提出的公式测定中国草芦对土壤及肥料中N、P、K三元素的吸收和利用系数,并测定植物粗蛋白质和粗脂肪的含量,成熟期测定鲜草、干草产量和种子产量,结果表明:中国草芦对土壤中N的吸收系数(Kn%)以PK处理最高为12.36,K处理降低。对肥料中N的利用系数(Ky%)以NPK处理为最高(14.78),所有处理均高于对照。对土壤中P的吸收系数Kn%以NK处理最高为19.30,K处理的降低。对肥料中P的利用系数Ky%,以NP最高为19.52,所有处理均比CK高,对土壤中K的吸收系数Kn%以NP处理最高,为19.2,单一的P处理最低,对肥料中K的利用系数Ky%以NPK处理最高,为28.44。鲜草和干草产量以NP处理最高,且所有处理均高于对照。种子产量及粗蛋白质含量以NK处理最高,粗脂肪含量以NK、N处理最高,本实验说明中国草芦进行人工栽培后,施用一定比例的肥料,可以提高肥料的利用效率,提高产量的品质。     

《河北植物志》第1卷柳属植物补遗

对河北省的柳属（ＳａｌｉｘＬ．）植物进行了多年的调查研究，考证了有关文献，经过鉴定，现知河北柳属有３４种４变种３变型，比《河北植物志》中增加了１７种１变种，本文进行了补遗，并讨论了相近种的区别特征。     

球毛壳菌对樟子松的侵染规律研究

榆林风沙区樟子松枯死主要发生在10—15年生幼龄林上，7月上旬至8月下旬为感病高峰期，翌年4月中旬至5月中旬为死亡高峰期。病菌孢子在整个生长季均能放散与传播，降雨节律及降雨量直接影响孢子的扩散与数量。人工接种发现：病菌的侵入期主要在6月中旬至8月中旬，潜育期半个月，侵入途径是伤口，病株感病部位及枯死枝是病原菌的越冬场所，密度高，植被盖度大的林分感病重。     

河北植物新增补属、种与入侵物种新分布

报道了《河北植物志》与《河北高等植物名录》未记录的植物1属3种1变种。其中拔毒散(Sida szechuen-sis Matsuda)、偃狗尾草[Setaria viridis(L.)Beauv. var. depressa(Honda)Kitag.]在河北省首次记录。     

大豆多肽对西洋参生物生长量与人参皂苷Rb1含量的影响

研究大豆多肽对西洋参(Panax quinquefoliusL.)生物生长量和人参皂苷Rb1含量的影响。将大豆多肽2号粉和3号粉分别配制成高、中、低3种浓度的水溶液,以叶面喷洒与根部灌注的方式,对生长3年的西洋参进行实验;同时观察生物生长量和人参皂苷Rb1的变化并总结规律。大豆多肽2号粉4mg/ml浓度根部灌注对西洋参生物生长量与人参皂苷Rb1增加作用最明显。研究结果为提高西洋参产量与质量提供理论依据,在西洋参的栽培种植中起到了实践指导作用。