玉米杂交种品玉8号的选育

品玉8号是山西省农科院品种资源研究所以自选系品364为母本,品578为父本,与2000年育成的玉米单交种,经多年试验示范结果表明,该品种具有早熟、抗病、高产、优质、适应广等特点。     

Application of a recombinant protein for the serological diagnosis of canine leishmaniasis

This work reports the results obtained by a new enzyme-linked immunosorbent assay (ELISA) test developed for the serological diagnosis of canine leishmaniasis.The new ELISA is based on a recombinant protein obtained by joining different antigens of Leishmania infantum.Test performances have been evaluated through the screening 227 sera of dogs, infected and uninfected by L. infantum. The new ELISA test has been compared to the indirect immunofluorescent-antibody test (IFAT) as a reference assay of canine leishmaniasis, and to a commercial ELISA.Excluding from the total number of IFAT positive sera the 27 sera with IFAT titre 1:40 (considered doubtful), the recombinant ELISA showed 97.0% specificity, 93.9% sensitivity and 95.5% agreement with IFAT. The commercial ELISA showed 78.2% specificity, 94.9% sensitivity and 86.5% agreement with IFAT.The results demonstrate a higher performance of the new recombinant ELISA test for the detection of negative samples, with a greater agreement with the reference test (IFAT).     

昭通不同种植模式对苹果‘烟富8号’树体生长及 果实品质的影响

摘 要：【目的】明确烟富8号在昭通地区适宜的种植模式,为昭通地区烟富8号在种植模式、科学管理上提供理论依据。【方法】本试验以M9-T337矮化自根砧嫁接烟富8号、八棱海棠上嫁接M9-T337并嫁接烟富8号、八棱海棠上嫁接烟富8号为材料。调查对比不同种植模式对苹果树生长的影响。【结果】不同种植模式对烟富8号树体长势、早果丰产性影响差异显著,变异系数为9.13%~94.12%。矮化自根砧显著提高烟富8号的中短枝比例,显著降低2年生树体冠幅,显著提高2年生、3年生的单株产量及亩产量;矮化中间砧显著降低烟富8号树体主枝数、总枝量及短枝比例;乔砧提高了烟富8号长枝比例,显著降低2年生、3年生的单株产量及亩产量。不同种植模式对烟富8号果实品质影响差异不显著。【结论】矮化自根砧在控制果树长势、促进早果丰产方面综合评价最佳。     

基于地表高光谱与OLI影像的土壤含盐量和pH值估测

针对宁夏银北地区大面积土壤盐碱化监测的需要,利用实测植被冠层光谱与Landsat 8 OLI影像相结合进行土壤含盐量和pH值估测研究.对实测植被冠层高光谱与影像多光谱反射率进行倒数、对数、三角函数及其一阶微分等一系列变换,确定最佳光谱变换形式,筛选敏感植被指数和敏感波段,分别建立基于实测植被光谱与Landsat 8 O...     

优质加工型裸燕麦新品种坝莜8号选育及推广应用

坝莜8号是以皮、裸燕麦种间杂交育成的裸燕麦高代品系品2号为母本,以8711-40-3为父本,采用裸燕麦品种间有性杂交和系谱法选育培育而成的裸燕麦新品种,其高度聚合了皮、裸燕麦高产、高β-葡聚糖、低脂肪、低皮燕麦率的优良性状。加工出的面粉口感好,在乌盟作为注册品牌燕麦粉大量销售。该品种于2013年10月通过了河北省科学技术厅组织的成果鉴定(20132743),现已大面积应用于生产。     

基于Landsat和MODIS数据融合的农牧区NPP模拟

天山北坡是中国重要的农牧业发展基地,利用遥感数据准确获取植被净初级生产力(Net primary productivity,NPP)的时空信息,对于合理分配农牧业草地资源具有重要意义。由于受到天气影响及卫星传感器受到时间分辨率和空间分辨率的限制,获取既具有中空间分辨率、又具有高时间分辨率的遥感数据比较困难。本文基于中空间分辨率Landsat 8 OLI数据与高时间分辨率MODIS数据,采用遥感数据时空融合STARFM算法,获取中空间分辨率和高时间分辨率序列的遥感数据,以天山北坡中段区域为实验区,结合CASA模型,对区域内植被NPP进行模拟。结果表明,2016年内8个时期,融合后的NDVI数据与对应时刻的Landsat 8 OLI NDVI数据的相关系数不小于0.759,偏差在0.006 2～0.009 4之间,均方根误差在0.074～0.135之间;利用融合数据与CASA模型协同模拟的NPP具有良好的空间细节信息,NPP模拟值与野外实测值决定系数R~2为0.860 1,表明两者具有较好的相关性。本研究为多源遥感影像融合技术与光能利用率模型协同模拟NPP提供了新的思路。     

血清8型鸭疫里墨氏杆菌在我国的发现及其病原特性研究

1994年1月-2000年10月，从全国23个省（市，自治区）不同代次（原种，祖代，父母代和商品代）的5-90日龄患有典型鸭传染性浆膜炎的病死鸭分离到68株血清8型的鸭疫里墨氏杆菌，对其病原学特性进行研究的结果表明，血清8型的鸭疫里墨氏杆菌在我国鸭群感染分布的范围极广，分离菌株对雏鸭具有很强的致病性，对小鼠无致病性，各地分离菌株表现出一致的形态特征和非常相似的生理生化特性；各地分离菌株耐药谱很广，但对头孢类药物（如头孢拉啶，头孢克洛等）和利福平却普遍高度敏感。用分离菌株制备的灭活疫苗免疫雏鸭具有良好的免疫原性。     

Effects of two commercial diets and technical feed treatment on stomach lesions and immune system of fattening pigs

The impact of technical feed treatment and diet on stomach lesions and traits of the local and systemic immune system were investigated in fattening pigs. Feeding groups differed in technical feed treatment (standard ground meal vs. finely ground and pelleted feed) and diet (soya bean meal vs. rapeseed meal/DDGS/soya beans). Pigs were fattened approximately 10 weeks by ad libitum feeding and slaughtered subsequently. Gastric alterations were assessed by a macroscopic scoring system [macroscopic stomach score (MSC) 0 =  normal to 4 =  severe lesions]. For immunological investigations, lymphocytes from blood and jejunal tissues were isolated. T‐cell phenotyping was carried out by staining intestinal lymphocytes with monoclonal antibodies for CD4 and CD8 and flow cytometric measurements. MSC was higher in animals fed finely ground and pelleted feed compared with their counterparts. Significant interactions between diet and feed treatment considering the MSC were observed (p = 0.027). There was no effect of diet or technical feed treatment on T cells of blood, Lymphonodi gastrici or lamina propria (LP) and intraepithelial cells. However, technical feed treatment significantly affected subsets of CD4⁺, CD8⁺, CD8^low, CD4/CD8 double‐positive T cells, the mean fluorescence intensity of CD4⁺ T cells and the ratio of CD8^low/CD8^high T cells in Peyer's patches (PP). All named parameters were reduced in PP of animals fed finely ground and pelleted feed compared with animals fed standard ground meal. Furthermore, significant differences between T cells of lymph nodes and LP were observed between animals with middle MSC (MSC = 1–2.5) and animals with high MSC (MSC = 3–4). Significant alterations in T cells of PP were observed between animals of low (MSC = 0–0.5) and high MSC. The observed effects provide the evidence that the impact of technical feed treatment is not limited on the stomach lesions. Possible stimuli and consequences of the immune system should be studied in more detail.     

Functional characteristics of porcine peripheral T cells stimulated with IL-2 or IL-2 and PMA

In human or mouse, mature T cells express either CD4 or CD8, resulting in different functions in the periphery. Interestingly, porcine CD4 and CD8 double positive (DP) T cells are present in the blood, and their proportions change from youth to adulthood. However, the features of these cells in swine are poorly understood. We investigated the fate of porcine peripheral T cells based on their functional characteristics, including proliferation and the expression of CD4 and CD8 co-receptors. The results showed that all the populations changed their CD8 expression in a time-dependent manner and porcine T cells had different proliferative pattern from human T cells. The results further revealed that Th2 cytokines were increased later in porcine T cells compared to human T cells upon stimulation with IL-2 + PMA. Collectively, we found that the fate of porcine peripheral T cells is different from that of human T cells, and the changes occur in a time- and stimulation-dependent manner.     

拮抗细菌Enterobacter cloaeae B8的一株抗菌素抗性突变体(英文)

为了研究拮抗细菌Enterobacter cloacae B8在野外水稻叶面上的定殖情况及拮抗作用,由自然选择和Tn5诱变从B8产生了一株抗菌素抗性突变体BX8.BX8能在含利福平达400μg/ml或卡那霉素达300μg/ml的LB培养基中生长.实验表明该抗菌素抗性的产生没有降低BX8的拮抗活性.该抗性比较稳定,在无抗菌素培养基中培养.BX8至少分裂生长60代内抗性不变.