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51.
利用DD-RTPCR(正常鲤鱼外周血白细胞和经有丝分裂源刺激后白细胞的总RNA)获得的差异显示片段B10克隆(编码IL8的一段序列),对该基因进行DIG标记,对有丝分裂原刺激的鲤鱼外周血白细胞cDNA文库进行核酸杂交筛选,从0.8×104个重组噬菌体中,经过2轮筛选最终获得阳性克隆,挑选了3个分别命名为IL8-1,IL8-2,IL8-3。测序后经NCBI Blast分析表明,阳性克隆具有完整阅读框架,均为编码鲤鱼IL8的全长cDNA,编码的多肽由98个氨基酸残基组成,其相对分子质量理论推导值为 10 848.9 Da,等电点为8.68。且与2003年12月Dev Comp Immunol.杂志报道的荷兰鲤鱼氨基酸的同源性达84%。 相似文献
52.
好运8号是广西农科院园艺研究所为适应南方湿热地区厚皮甜瓜栽培对品种的要求,利用逆环境定向选育,以自交系HN14-d作母本,TY8-a作父本杂交配制成的一代杂种。果实椭圆形,皮色橙黄有绿色斑块,网纹中等,一般单瓜重2.0kg,果肉橙红偏黄,皮肉厚约3.5cm,质地脆嫩蜜甜,有淡香,口感佳,中心可溶性固形物含量15~16%,全生育期春茬110d左右,秋茬90d左右,果实发育期45d左右,商品性好,一般产量36t/hm2,植株长势较强,易座果,较耐白粉病、霜霉病,抗蔓枯病能力中等,适于南方地区保护地种植。2007年3月通过广西农作物品种审定委员会审定 相似文献
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广丰香8号是广州市农业科学研究院育成的优质高产丝苗型香稻品种,2009年1月通过广东省品种审定,2012 ~2014年连续3年被遴选为广州市农业主导品种.该品种米质优,达国标优质2级、省标优质2级,并且叶、茎、米均有清香味,饭软、滑,口感好,冷饭不回生,适合加工高档丝苗米和香米;丰产性较强,于2012 ~2014年连续3年纳入万亩连片示范区示范,2012、2013年经实割测产平均产量在7 500 kg/hm2以上.介绍该品种的特征特性和高产栽培技术. 相似文献
55.
Chinese urban migrants' sense of place: Emotional attachment,identity formation,and place dependence in the city and community of Guangzhou 总被引:2,自引:0,他引:2
In this paper, we study urban migrants' sense of place in Guangzhou, China, focusing on the structural relations between place attachment, identification and dependence as the three key place dimensions. Through both quantitative structural equation modelling and qualitative analysis of in‐depth interviews data, our research suggests that migrants' sense of place demonstrates complex relationships between the three registers of emotional attachment, identity formation and functional dependence. The construction of sense of place is also related to the personal experiences of living as urban ‘outsiders’. Our research also reveals a striking difference between the city and community levels in terms of the ways in which migrants' sense of place is constructed. Urban migrants tend to exploit the functional utilities of microscopic urban spaces to meet their demands for recreation, education and socialisation. On the other hand, their sense of place to the city is largely compromised by their attachment to the hometown and conditioned by their personal identification to the city. 相似文献
56.
Takayama I Kubo M Takenaka A Fujita K Sugiyama T Arai T Yoneda M Sato H Yanai T Kai C 《Comparative immunology, microbiology and infectious diseases》2009,32(6):539-549
Ten wild masked palm civets infected with canine distemper virus (CDV), captured in Japan from 2005 to 2007, were histopathologically and phylogenetically analyzed. Phylogenetic analysis based on the amino acid sequences of the H protein of two CDV isolates from masked palm civets revealed that the two isolates were classified into the clade of recent isolates in Japan. Histopathologically marked lesions of virus encephalitis were present in the brain, whereas gastrointestinal lesions were absent or at a mild degree. The distribution of the lesions resembles that of recent CDV cases in dogs. Therefore, recent CDV infections in masked palm civets could be caused by recently prevalent CDV in dogs. The possibility of the masked palm civet as a spreader of CDV among wildlife is also discussed. 相似文献
57.
Kurane I 《Comparative immunology, microbiology and infectious diseases》2007,30(5-6):329-340
Dengue virus infections are a serious cause of morbidity and mortality in most tropical and subtropical areas of the world; Southeast and South Asia, Central and South America, and the Caribbean. Dengue virus infection can be asymptomatic or causes two forms of illness, dengue fever (DF) and dengue hemorrhagic fever (DHF), which is the severe form of dengue illness and often fatal. Pathogenesis of DHF has been analyzed, and two mechanisms are considered to be responsible. These include dengue serotype cross-reactive immune responses and virulence of the virus. The immunopathological mechanisms include a complex series of immune responses. Rapid increase in the levels of cytokines, especially TNF-, and chemical mediators play a key role in inducing unique clinical manifestations of DHF such as plasma leakage, shock, and hemorrhagic manifestations. It is understood that the process is initiated by infection with a virulent dengue virus, often in the presence of antibodies that enhance dengue virus infection in secondary infection, and then triggered by rapidly elevated cytokines and chemical mediators that were produced by intense immune activation. However, complete understanding of the entire pathological mechanism is far from complete, and further studies are still needed. 相似文献
58.
59.
Nattaya Lourith Takeshi Katayama Kimiko Ishikawa Toshisada Suzuki 《Journal of Wood Science》2005,51(4):379-386
To investigate the biosynthesis and stereochemistry of syringylglycerol-8-O-4′-(sinapyl alcohol) ether (SGSE), a syringyl 8-O-4′ neolignan, feeding experiments and enzyme assays using Eucommia ulmoides were carried out. Diastereoselective formation of erythro-SGSE was found. When [8-14C]sinapyl alcohol was administered to excised shoots of E. ulmoides, 14C was incorporated into free SGSE and SGSE glucosides. In stems, incorporation into (+)-erythro-[14C]SGSE (0.037%) with 9.1% enantiomeric excess (% e.e.) was found; incorporation into the threo isomer was not detectable. Erythro-[14C]SGSE glucosides (0.047%) dominated over threo forms (0.007%) with 74.0% diastereomeric excess (% d.e.); both diastereomers were levorotatory with 32.0% e.e. and 18.3% e.e., respectively. In leaves, higher incorporation into (−)-erythro-[14C]SGSE (0.500%, 15.9% e.e.) than into the threo isomer (0.206%, 7.4% e.e.) was observed (41.6% d.e.). (−)-Erythro-[14C]SGSE glucosides (1.692%, 25.0% e.e.) were produced at higher rates than threo isomers (0.177%, 16.4% e.e.) with 81.0% d.e. In incubations of a mixture of [8-14C]sinapyl and [8-14C]coniferyl alcohols with an insoluble enzyme preparation from stems of E. ulmoides, erythro-SGSE was preferentially produced. The highest % d.e. (82.8) was observed at 60 min with the (+)-erythro isomer (21.4% e.e.) and the (−)-threo form (4.3% e.e.).Part of this report was presented at the 52nd Annual Meeting of the Japan Wood Research Society, Gifu, April 2002, and the 47th Lignin Symposium, Fukuoka, October 2002 相似文献
60.
Sugi (Cryptomeria japonica D. Don) wood was treated with supercritical water (374°C, 22.1MPa), and fractionated into the water-soluble portion, the methanol-soluble portion, and the methanol-insoluble residue. The methanol-soluble portion mainly consisted of the lignin-derived products. To characterize the compounds in the methanol-soluble portion, gel permeation chromatographic (GPC) and gas chromatographic-mass spectrometric (GC-MS) analyses were performed. The GPC analysis indicated that the methanol-soluble portion contained lignin-derived monomeric and dimeric products. GC-MS analysis detected 31 products which were expected to be monomeric compounds, and 18 of these were identified to be guaiacol, methylguaiacol, ethylguaiacol, vinylguaiacol, eugenol, propylguaiacol, vanillin, cis-isoeugenol, homovanillin, trans-isoeugenol, acetoguaiacone, propioguaiacone, guaiacylacetone, 2-methoxy-4-(1-hydroxypropyl)phenol, homovanillic acid, 2-methoxy-4-(prop-1-en-3-one)phenol, coniferyl aldehyde, and ferulic acid. In addition, 22 dimeric products were detected, and 4 of these were believed to be compounds with biphenyl type (5-5), diphenylethane type (-1), stilbene type (-1), and phenylcoumaran type (-5) structures. These results clearly indicated that the methanol-soluble portion included various monomeric and dimeric compounds produced as a result of the cleavage of ether linkages and propyl chains of lignin. 相似文献