60Co诱变方法选育高产多糖槐耳菌株

采用槐耳T菌株作为诱变材料,进行60Co射线的诱变试验（剂量为1000Gy,剂量率为10Gy·min^-1）,筛选出多糖含量高且适合栽培的新菌株T189,其多糖产量为37．13％,比槐耳T菌株（27．48％）提高35．1％。     

可移植的UART底层驱动构件设计

以飞思卡尔公司基于ARM Cortex-M4内核的K60系列微控制器为例,设计了一种具有较好可移植性和重用性的UART底层驱动构件．测试结果表明该构件正确而有效．     

Aerococcus viridans expression of Cpn60 is associated with virulence during infection of the American lobster, Homarus americanus Milne Edwards

The Gram-positive bacterium Aerococcus viridans var. homari is a well-documented causative agent of the lethal systemic disease gaffkemia in both the American lobster, Homarus americanus, and the European lobster, Homarus gammarus. Previous phenotypic characterization has been unsuccessful at differentiating avirulent from virulent strains without performing lethal animal infection trials. Recent genetic characterization of A. viridans strains through 16S rRNA sequencing and random amplification of polymorphic DNA fingerprinting has revealed the presence of two subtypes. However, subtype 1 contains both virulent and avirulent strains which are genetically identical. The purpose of this study was to determine the proteomic mediators of virulence in A. viridans. Quantitative proteomic mapping of these two strains has revealed 29 differentially expressed protein spots, seven of which are only expressed in the virulent strain and could act as virulence factors. One protein, chaperonin 60 (Cpn60), is uniquely expressed in the virulent strain and has been shown to act as a virulence factor in many other bacteria. The proteomic mapping strategy employed in this study is the first to show phenotypic differences between virulent and avirulent strains. Cpn60 expression represents a potentially useful tool for identifying the virulent strains of A. viridans in epidemiological studies.     

^60 Co-γ射线辐射对苋菜种子发芽率及幼苗生长的影响

用不同剂量的^60 Co-γ射线对苋菜种子进行辐射，观测其对苋菜种子发芽率和幼苗生长的影响。结果表明，在该实验设计的辐射剂量范围（20-200Gy）内，对苋菜种子有刺激生长的作用，20Gy有利于苋菜种子发芽，60～80Gy有利于苋菜幼苗的生长。     

丙氨酸-ESR剂量计用于测量产品箱内剂量分布的研究

本文用自制丙氨酸-ESR剂量计和西德辐射与环境研究中心(GSF)的丙氨酸-ESR剂量计,测量了北京市射线应用中心的~(60)Coγ辐射源(1.44×10~(16)Bq)辐照纸箱包装(913mm×555mm×460mm)产品箱时的吸收剂量分布,箱内模拟产品(报纸)的堆积密度为200ks/m~3,测量结果表明,产品箱內最大剂量D_(max)与最小剂量D_(min)之比分别为1.45(北京市辐射中心)和1.47(GSF),两者相差1.40％,在允许误差范围之内。     

HL-60 Cells Apoptosis Induced by Low-dose Cytosinearabinoside in Vitro

Cytosinearabinoside(Ara-C) is an important agent used for treatment of leukemia, but its mechanism of action at low dose is not clear, maybe is related to its effectson differentiationor apoptosis of cells. The authors investigate The relationship between bcl-2, p53 expression and apoptosis of HL-60 cells induced by low-dose cytosinearabinoside (10~(-8)M) by means of TUNEL method, flow cytometry, immunohistochemistry and in situ hybridization. The results show that low-doseAra-C could inhibit cell growth and induce apoptosis, and the effects might be related to increase of P53protein and decrease of bcl-2 mRNA expression.     

60Coγ射线种子辐射对大花高代 组培苗的生长及诱变效应

以0Gy、10Gy、50Gy、100Gy、150Gy和200Gy不同剂量的60Coγ射线辐射种子，研究其对大花高代组培苗生长影响的结果表明，在50~200Gy范围内，根和真叶的生长以及植株高度都不同程度地受到了抑制，且抑制程度与辐射剂量呈正相关，而10Gy则能促进根和真叶的生长。随着剂量的增高，死亡率增高，组培苗生长41d时的半致死剂量(LD50)为150Gy。各处理组培苗培养70d后，少量瓶内开花，出现花型、花色和花瓣变异。花型变异主要由对照的正常花形变为蝴蝶形。花色变异主要是颜色变浅，甚至变为白色。花瓣变异可由原来的单瓣变为重瓣花。以上结果表明，60Coγ射线辐射可以提高突变频率且诱变效应明显，有利于在大花高代育种中产生色彩丰富，花型、株型美观的变异类型     

~(60)Co辐照诱变对姬松茸菌丝生长及其细胞形态结构的影响

本文报道了经不同剂量60 Coγ射线辐照对姬松茸菌丝生长、扭结和细胞形态结构的影响。结果表明 ,采用 0 2～ 0 5kGy低剂量辐射后的姬松茸菌丝 ,细胞壁比对照厚 ,而且细胞出现重度质壁分离。随着辐照剂量增大其细胞壁则变薄 ,而且细胞出现轻度质壁分离。经高剂量辐射后 ,姬松茸菌丝生长速度减缓 ,菌丝生长呈稀弱状 ,但与对照相比 ,采用低剂量辐射则有利于促进菌丝生长和提前扭结 ,其子实体增产率达 34 8%。     

~(60)Coγ射线诱发梨的短枝型变异的探讨

本文对苹果梨、朝鲜洋梨辐射营养后代的短枝型变异进行了研究。研究结果表明:辐射有利于培育短枝型品种。在本试验范围内,辐射处理均有不同程度的矮化,而且与对照差异极显著。朝鲜洋梨较苹果梨对辐射处理更敏感。采用辐射诱变矮化型品种,可在V_1代直接进行选择。     

60Co-γ射线对菊花组培苗的诱变效应

对菊花(Chrysanthemum morifolium Ramat.)品种秋之山的组培苗, 采用5～30 Gy的60Co-γ射线照射处理。研究了γ射线对组培苗生长、增殖和生根的影响以及对组培再生植株移栽后生长开花的影响。结果表明20 Gy是γ射线处理菊花组培苗的致死剂量；从生根和继代培养生长和增殖的结果看，诱变处理的最佳剂量在10 Gy左右；组培再生植株移栽后发现，10 Gy处理的植株中花色变异率为8.2%。与对照花鲜艳的纯黄色相比，变异花增加了不同深浅的红色素，且突变植株均为同质稳定突变。