极早熟大白菜新品种豫新50的选育

采用游离小孢子培养双单倍体技术快速育成早熟大白菜杂交一代新品种豫新50。结果表明:豫新50为极早熟耐热品种,从播种到采收50-56d,高抗病毒病和软腐病,抗霜霉病;平均667m2产净菜2716.87kg,株形紧凑,适宜密植;叶球矮桩叠抱,整齐度高,商品性好;质地柔嫩,风味佳,每100g鲜样含粗蛋白质1.19g,粗纤维0.40g,维生素C20.8mg,可溶性糖2.80g。     

鸡传染性腔上囊病病毒VP2基因在昆虫细胞中的表达

为了深入研究鸡传染性腔上囊病病毒（IBDV）VP2基因的结构和功能,利用Bac-to-Bac系统研制出含有VP2基因的重组杆状病毒rBac-VP2,将rBac-VP2感染Sf9细胞,并用抗IBDV VP2特异性单克隆抗体经间接免疫荧光试验检测,证实感染重组病毒Sf9细胞能高效表达IBDV VP2基因产物;Western-blotting分析结果表明,VP2基因表达产物的分子质量约为40 ku.     

辣椒新品种镇江3号的选育

中晚熟杂交一代辣椒镇研3号是以江苏本地羊角椒自交系Y9007为母本,由甘肃酒泉引进的甜椒自交系T9218为父本配制的一代杂交组合。镇研3号为中熟辣椒品种,果形为牛角形,纵径15cm,肩横径5.0cm、肉厚0.40cm,单果平均重42g。果面光滑,青熟果绿色、味微辣,风味佳,适宜在长江流域或南方保护地栽培种植,667m2产量约为4200～5000kg。     

珍黄88(湘椒29号)辣椒的选育

珍黄88的母本6427是从广西地方品种经自交纯化而成的优良黄皮尖椒自交系;父本8215为叶少、部分茎节短缩、肉厚疏松的辣椒高代自交系。该品种中熟,定植至采收青椒约50 d(天),产量2500～2800 kg·(667 m~2)~(-1),抗病毒病,中抗疫病,适于山东、广东等黄皮尖椒外运基地栽培;果实长牛角形,果表黄绿无皱,果型直,肉厚腔小,商品性好,耐贮运。     

日光温室黄瓜济杂3号的选育

母本88001来源于津杂2号,父本88010来源于津研2号与新泰密刺的杂交后代。其一代杂种济杂3号,瓜条顺直,长30～35 cm,单瓜质量约180 g,瓜把短,瓜皮深绿,密瘤白刺,品质优,商品性好,对霜霉病、白粉病、枯萎病抗性强。每667m~2产量7000～14000 kg,适合日光温室越冬茬和早春茬栽培。     

春丰50苦瓜的选育

以曼谷青皮、碧绿1号和钦州大肉苦瓜为育种材料,采用双父本混合花粉杂交和后代系谱选择育成优良自交系,再经配合力测定选出优良一代杂种春丰50。该品种生长势强,果实粗棒形,纵径26.3cm,横径6.45cm,皮色翠绿油亮,一般单果质量420g左右。平均每667m~2产量3 500kg。     

Relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats

AIM:To elucidate the relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats (SHR).METHODS:Intracellular free calcium concentrations were measured by Fura 2 methodology and left ventricular function quantitated by cardiac catheterization in 20 SHR aged 10, 22, and 34 weeks and 20 age-matched Wistar-kyoto (WKY) rats.RESULTS:(1) The systolic blood pressure(SBP), intracellular calcium concentrations and left ventricular mass / body weight index (LVM/BW) were significantly higher in all three age groups of SHR than the corresponding groups of WKY; (2) Compared with age-matched WKY groups, the peak left ventricular pressure descending rate(-dp／dt_max) decreased while left ventricular relaxation time constant (τ)increased significantly in SHR aged 22 and 34 weeks. The peak left ventricular pressure ascending rate(dp／dt_max) and the left ventricular contractility index were significantly increased only in the 34 weeks SHR; (3) Intracellular calcium concentrations showed a positive correlation with LVM/BW,SBP,-dp／dt_max and τ(r=0.47-0.83,P<0.01)and a negative correlation with dp／dt_max and the left ventricular contractility index (r=-0.46,P<0.05 and r=-0.81, P<0.01).CONCLUSION:Intracellular calcium overload is one of the potential mechanisms in the induction of left ventricular hypertrophy as well as of systolic and diastolic dysfunction.     

Quick detection of sex determining region protein gene in mouse fetuses

AIM: To detect quickly the Y-chromosome specific sex determining region protein (Sry) gene in mouse fetuses on embryonic day 14.5 with a PCR method. METHODS: We designed specific primers with the OLIGO 5. 0 software. Templates were prepared in 30 minutes by the following way. About 1 mg embryonic tissue but not fetal liver was suspended, and treated with 200μL of lysis buffer, consisting of PCR buffer containing 20 mg/L proteinase K, 0. 5% NP-40, and 0.05% Tween 40, at 60°C for 15 minutes, heated for 5 minutes at 100 °C, 10μL was used as template. The PCR react ion was performed in 50μL, using two sets of primers specific for Sry gene (chromosome Y) and IL-3 gene (chromosome 11) . PCR conditions and cycle numbers were optimized. The assessment of the results was done by electrophoresis in 3% agarose run at high voltage. The specificity of the method was conf irmed by fluorescent in situ hybridization (FISH) using a specific male probe on embryonic tissue cells. RESULTS: Electrophoresis showed that PCR product of male control DNA consisted of a 649 bp product representing the IL-3 gene and a 444 bp product representing the Y-specific Sry gene, female control DNA only one 649 bp product. Fetuses with two bands matching those as seen inmale control DNA are the presumpt ive male fetuses. Fetuses, only the IL-3-associated 649 bp band, are the presumptive female fetuses. These were confirmed by FISH. The ent ire procedure took <3. 5 h. CONCLUSION: The established PCR assay offers a quick, simple, accurate, and sensitive detection of sex determining region protein gene in mouse fetuses. This method allowed the preparation and culture of pure male and female hematopoietic stem cells from fetal tissue.     

Sca-1+ cells from mouse fetal liver can differentiate into neurons in vitro

AIM: To study whether Sca-1⁺ cells from fetal liver can be induced to differentiate into neuronal cells in vitro. METHODS:Sca-1⁺cells from 14 5-days-old murine fetal liver were isolated with a magnetic cell sorting kit, and were cultured in Dulbecco s modif ied Eagle s medium(DMEM)/F12 supplemented with 10%fetal bovine serum(FBS), and passaged at a rat io of 1 3 when cells reached more than 80%confluence.The 5 passage cells were induced by 10^-3mol/Lβ-mercaptoethanol(β-ME)and 5×10^-7 mol/L all-trans-retinoic acid(RA)for 24 hours, and then incubated in serum-free medium for 5 hours to 5 days.The characteristics of treated cel s were assayed by immunocytochemistry staining analysis at 5 hours, or 5 days.RESULTS: Cells treated with β-ME and RA exhibited neuronal phenotype and expressed neuron-specific protein such as neuron-specific nuclear protein (NeuN), neuronfilament-M, and neuron-specific tubulin-1 (TuJ-1) but not tau, MAP-2, or the astrocyte-specific marker glial fibrillary acidic protein (GFAP).CONCLUSION: Sca-1⁺ cells from fetal liver, of which most are regarded as hematopoietic stem cells, could differentiate into early immature neuronal cells in vitro. These findings suggest that Sca-1⁺ cells from fetal liver may be an alternative source in cell therapy and gene therapy of neural dysfunction.