矮牵牛的快速繁殖技术

以矮牵牛为试材,筛选了快速繁殖优良苗木时的最适基本培养基浓度及激素条件．结果表明分化培养时的最适培养基为１／４ ＭＳ＋ ＢＡ０ ．５ ｍｇ／Ｌ＋ＩＢＡ０ ．０２ ｍｇ／Ｌ;增殖培养时为ＭＳ＋ ＢＡ０ ．５ｍｇ／Ｌ＋ ＩＢＡ０ ．２ ｍｇ／Ｌ;诱导根系培养时为１／２ ＭＳ＋ ＩＢＡ０ ．５ ｍｇ／Ｌ＋ 活性炭１００ ｍｇ／Ｌ．生根培养基中添加活性炭有效地促进了矮牵牛根系的生长和发育,同时促进了叶片和茎的分化生长．     

玉米自交系Ⅱ型胚性愈伤组织诱导及遗传转化初报

以玉米自交系３４０芽尖、４１１２未成熟幼胚为试验材料,诱导出良好的Ⅱ型胚性愈伤组织;用这２种材料的Ⅱ型胚性愈伤组织为受体,通过基因枪轰击法将Ｂａｒ基因转入玉米细胞,然后用ＧＵＳ基因检测。结果为阳性。     

蛹草菌菌丝体培养条件的选择

不同培养基、环境因素（温度、ｐＨ、光照）、营养条件（碳源、氮源）对蛹草菌菌丝体生长影响的研究结果表明：蛹草菌菌丝体在ＰＤＡ综合培养基和沈农５号培养基上生长状况最好，在ＰＤＡ培养基、ＰＤＡ麸皮培养基生长最差．菌丝体在１５～２５℃范围内均能生长，其中在２５℃条件下生长状况最好，而且随着温度的升高，生长状况明显增强，３０℃和件下生长成侵，３５℃停止生长．菌丝体对酸碱度要求不严，在ｐＨ５．０～８范围内均能生长，其中在ｐＨ６．５～７．０生长最好．菌丝体在非光照条件下比在光照条件下生长旺盛．对不同碳源、氮源吸收各异，其中以甘露醇为碳源，以牛肉膏为氮源生长状况最佳．     

高校图书馆为中小企业服务初探

本文分析了高校图书馆为中小企提供信息服务的必要性和可行性，探讨了信息服务的方式。     

热媒炉燃料油预热温度的分析和控制

对热炉燃烧油预热流温度的控制是热炉炉提高炉效、稳定运行的关键，也有降耗的有效途径。以东管于黄岛输油管道中东营、寿光、昌邑这三个砂站采用的ＣＥ－ＮＡＴＣＯ热煤炉为例，分析和计算了采用胜利原油和青岛渣 作了为热炉燃料时发控制的温度范围，并根据实际运行的经验，对的燃料油系统进行了改进。     

泗阳县农民专业合作经济组织发展调研与思考

农民专业合作经济组织是现代农业发展的重要支撑，对于加快农业产业化的发展、促进农村经济结构深度调整、提高农业组织化程度、培养新型农民和增加农民收入等方面具有重大战略意义。本文以泗阳县为例，分析了当前泗阳县农民专业合作经济组织发展状况及存在的主要问题，并提出相应对策和建议，期冀对推进苏北乃至全国同类地区农民专业合作组织发展起到一定的借鉴和推动作用。     

In vitro production of potato microtubers in liquid medium using temporary immersion

Summary CIRAD developed a new apparatus for plant tissue culture, using temporary immersion in a liquid medium. This apparatus was adapted to the microtuber production in potato. The procedure is as follows: single node cultivation on MS medium containing 30 g/l sucrose in the light for 2 weeks, induction of microtuberisation with 80 g/l sucrose over a 2 week period in the light, followed by a further 6 weeks in the dark. All experiments were performed at 20 °C. The basic vessel had a capacity of approximately 11;30 nodes were cultivated per vessel. Depending on the cultivars tested (Bintje, Ostara and Désirée) 47 to 115 microtubers were harvested per vessel. Between 30 and 60% of the microtubers weighted over 0.5 g and between 10 and 40% over 0.8 g. Sprouting is still under investigation. Preliminary results indicate that the dormancy period was relatively short and several stems were obtained per microtuber. These results seem to be better than those usually reported. Only one simple protocol has been tested and further improvements are probably easy to obtain.     

使用廉价培养基培养草鱼CIK细胞系

使用一种廉价的培养基 CAS 替代昂贵的 Eagle′S MEM 培养草鱼 CIK 细胞系,细胞生长迅速而稳定,在1.5—2天内可长成汇合单层。CAS 的最佳培养浓度为0.1%。     

半夏的组织培养

针对半夏繁殖材料珠芽采集困难，不能广泛栽培繁殖的障碍，开展了半夏组织培养的试验研究，已获成功。本文从取材、灭菌、接种培养、原块茎的继代培养、组培苗的移栽等方面探讨了半夏的组织培养技术和方法。并阐述了生长素与细胞分裂素对愈伤组织和小鳞茎形成的最佳配比浓度。在ＭＳ液体培养基上添０２５ｍｇ／ｌ浓度的２，４－Ｄ和Ｋｔ激素对半夏鲜重增殖率的提高最为适宜；在固体培养基中添加浓度为０５ｍｇ／ｌ的２，４－Ｄ和１ｍｇ／ｌ的Ｋｔ对半夏再生植株数目和组织鲜重提高有利。此外，还分析了在固体培养基中，添加不同浓度的６－ＢＡ、ＮＡＡ对半夏植株再生的影响，以及丛生苗分割的大小对半夏成活的影响。     

THE RADIOLOGICAL DIAGNOSIS OF THORACOLUMBAR DISC DISEASE IN THE DACHSHUND

The accuracy of survey radiographs in the diagnosis of acute thoracolumbar disc disease in 36 Dachshunds was determined by comparison with lumbar myelographic findings using iohexol. The value of making radiographs immediately after injection of contrast medium and the effectiveness of oblique radiographs in determining the exact circumferential distribution of extruding or protruding disc material were assessed. The presence of a double contrast medium column, resistance to injection and the presence of cerebrospinal fluid flow during needle placement was also evaluated. The location of the affected disc was accurately determined on survey radiographs in only 26 dogs. The myelographic technique used in this study resulted in the correct intervertebral space being identified, together with the exact circumferential distribution of disc material, in 35 dogs. Survey radiographs alone are inadequate for localization of protruding or extruding disc material.