冬小麦种质“矮孟牛”中新型小麦-黑麦复杂易位的遗传传递分析

本文利用矮孟牛中一种新型小麦-黑麦复杂易位IRS·7DS,1BL·7DL与1BL·1RS易位所构建的一套重组自交系(包括109个F6株系)和8个矮孟牛衍生品种进行了分析,结果表明在109个F6株系中,纯合复杂易位占69个,纯合1BL·1RS占36个,二者比例约2∶1,杂合类型占4个,出现频率4/109;对矮孟牛8个衍生品种的分析表明,6个品种包含来自矮孟牛的     

番茄果实中LeERF1、LeERF2基因的克隆及序列分析

乙烯反应元件结合蛋白属于植物特有的一个转录因子家族，这个家族保守的DNA结合域称为ERF结构域。根据对番茄(Lycopersicon esculentum)和其它植物物种中EREBP基因家族的同源性比较，在保守区(ERF区域)设计合成一对简并引物，通过RT-PCR扩增得到一个114bp的片段，用此片段作探针筛选番茄cDNA文库(粉红期)，得到两个基因LeERF1和LeERF2。通过BLAST工具在GenBank中搜索表明，LeERF1和LeERF2基因属于EREBP家族，LeERF1与EREBP-4和DDTFR10／A在氨基酸水平上的序列相似性为35％，LeERF2与EREBP-3和p65在氨基酸水平上的序列相似性为46％，是新的基因。LeERF1和LeERF2的核酸序列在GenBank发表，登录号分别为AY077626和AY275554。     

中央一号文件聚焦现代农业 水利农机板块料受益

据新华社消息,中共中央、国务院近日印发《关于加大改革创新力度加快农业现代化建设的若干意见》。在连续12年聚焦"三农"的同时,今年中央一号文件将"围绕建设现代农业,加快转变农业发展方式"放在首位。市场人士分析,随着"现代农业"的上位,水利、农机等现代农业板块在去年政策利好不断加码的情况下有望进一步迎来政策     

湖北移动“农机通”——全省百万农机手朋友平安致富的好帮手

<正>湖北移动"农机通",是由中国移动湖北公司和湖北省农业机械安全协会联合推出的一款应用于全省农机行业的手机通信服务产品,旨在为全省百万农机手朋友提供高效便捷的移动信息技术服务。一、使用"农机通"产品的三大优势:1.本地网内通话优惠,全省价格最低;2.手机上网流量优惠,全省价格最实惠;3.享受农机短信资讯服务,包括农机购置补贴政策、农机安全操作知识、农机维修保养常识和农机行业资讯等。     

《农机化研究》投稿须知

<正>为防止学术不端行为和进一步提高期刊文章质量,自2011年起,本刊特对所投稿件做如下要求,望作者周知。1、文章背景要求为基金项目,并给出资助背景名称和项目号;若非基金项目,需经本刊编委初审并推荐。2、文章最短篇幅为4版(双栏,每行22字,46行,宋体5号,Word2003),要求有引     

农业远程温湿度监测装置的设计—基于NRF24LE1和GPRS

农产品的质量与其生长环境和保存环境密切相关,而农业对象又具有分散偏僻的特点,因此研究便捷、低功耗、精确的远程温湿度监测装置具有现实意义。此装置由传感节点和中继节点组成,传感节点选用NRF24 LE1低功耗器件,负责感知环境的温湿度,通过2.4 GHz无线短距传输上传至中继节点;终端PC利用GPRS远距离与中继节点通信,实时周期性收集中继节点汇聚的传感数据。传感节点与中继节点间采用防碰撞延时发送机制,实现传感节点的低功耗和数据有效传输。中继节点与终端PC通过GPRS网络建立UDP连接,使系统可以管理多个中继节点,可扩展性好。     

《农机化研究》投稿须知

<正>为防止学术不端行为和进一步提高期刊文章质量,自2011年起,本刊特对所投稿件做如下要求,望作者周知。1、文章背景要求为基金项目,并给出资助背景名称和项目号;若非基金项目,需经本刊编委初审并推荐。2、文章最短篇幅为4版(双栏,每行22字,46行,宋体5号,Word2003),要求有引     

Cloning and Bioinformatic Analysis of L1 Gene of BPV from Guizhou Province

In order to study and analyze L1 gene of bovine papillomavirus（BPV）in Guizhou province,the L1 gene of BPV-GZ01 strain was amplified,cloned and sequenced using bioinformatic softwares and methods,and the secondary structure,tertiary structure,B-cell preponderant epitope,conserved domains analysis, transmembrane domain and signal peptide of L1 gene were predicted.The results showed that the length of L1 gene was 1 494 bp,encoding 497 amino acids.The L1 gene of BPV-GZ01 strain shared an amino acid identities of 98.6%,99.4%,98.4%,94.4% and 91.3%,and a nucleotide identities of 99.1%,99.8%,99.4%,87.6% and 82.8% with those of BPV2,BPV2-SW01,BPV2-AKS01,BPV13 and BPV1 strains,respectively.The results of phylogenetic tree analysis indicated that there was a close relationship between BPV-GZ01 and BPV2-SW01 strains.The prediction of secondary structure of L1 protein indicated that the random coil,extended strand and alphahelix took a higher percentage.The L1 protein was supposed contain 6 potential antigen epitopes.And no transmembrane domains and no signal peptide were found.The tertiary structure of L1 protein was curved spiral structure.These results provided a theoretical basis for immunologic diagnosis and further research of nucleic acid vaccine of BPV.     

Screening of Probiotics Inhibiting E. coli K88 in vitro

The probiotics tested in the experiment were isolated from the intestinal of weaning piglets.The isolated probiotics and E.coli K88 were inoculated into the culture of intestinal porcine epithelial cell-1 (IPEC-1).The activity of lactate dehydrogenase (LDH) in the supernatant was measured after incubating for 2.5 h.At the same time, the probiotics and E.coli K88 were co-cultured in vitro, the number of E.coli K88 was counted and the probiotics which could be resistant to the E.coli K88 were selected 2.5 h later.The results showed that the Lactobacillus casei and Bacillus coagulans could significantly reduce LDH activity (P<0.05), decrease the damage of E.coli K88; Bacillus coagulans could inhibit the growth of E.coli K88.At the same time, Bacillus coagulans could resist high temperature, acid and bile salt.The results showed that Bacillus coagulans strains had great potential as the application of probiotics strains.The methods could be used as a model of screen probiotics which could inhibit the growth of E.coli K88 in vitro.     

Study on Length Polymorphism of KAP1 Family Genes in Yak (Bos gurnniens)

This study was aimed to understand the characteristics of length polymorphism with repeat sequence of keratin associated protein 1 (KAP1) family genes in yak. KAP1 family genes of yak and cattle were sequenced, and compared with sheep KAP1 family gene sequences. The results showed that cattle KAP1 family genes were located in chromosome 19, according to location of sheep KAP1 family genes in the chromosome and similarity with cattle KAP1 family genes, renaming the cattle KAP1 family (according to the gene location of chromosome) B2D, B2A, KAP1-1 and B2C genes into KAP1-4, KAP1-1, KAP1-2 and KAP1-3 gene, respectively. KAP1 family genes in the 3'and 5' flank were highly conserved, the difference between family genes mainly in the the repeat sequence region, which yak KAP1 to KAP4 genes were found 30 bp length polymorphism. There were B(CCQTS)A₁(CCQPT) repeat sequence and a new repeat sequence C(SIQTS). The results indicated that the repeat sequence was the key of the polymorphism of KAP1 family genes, which might be relate to combination with keratin protein.