奶牛饲养管理要点

随着生活水平的不断提高,人们越来越重视饮食的营养价值,尤其是党和国家非常重视奶业发展工作。近年来,我国奶业发展很快,农户养奶牛也越来越多。为此,笔者简述奶牛饲养管理技术,以供奶牛养殖者参考。     

Cloning of Buffalo AQP9 Gene and Analysing its Expression in the Buffalo Tissues

Cloning buffalo AQP9 gene and analyzing its expression in buffalo tissues.A pair of primers was designed according to the released bovine AQP9 sequences in GenBank,which was used to clone buffalo AQP9 gene.The AQP9 gene was amplified by RT-PCR,whose nucleotide sequence and protein structure were analyzed by bioinformatics methods.The expression of AQP9 in buffalo tissues was assayed by Real-time quantitative PCR.The expression of AQP9 gene in buffalo ovary and testis tissue was detected by immunohistochemical staining method.The results showed that the cloned ORF length of buffalo AQP9 gene was 888 bp,which coded 295 amino acids.The results of multiple sequence comparison showed that the nucleotide sequence of buffalo AQP9 shared 99%,90%,97% and 88% homologeous compared with that of Bos taurus,Sus scrofa,Ovis ariessis and Homo sapiens,respectively,while shared 99%,86%,97%,83% homologeous for amino acids,respectively.Phylogenetic tree analysis indicated that AQP9 gene was highly conservative in the evolutionary process.Real-time quantitative PCR results showed that AQP9 gene expressed in buffalo liver,lung,brain,skin,testis and ovary tissues with different levels,had the most abundant expression in liver,followed by in skin and testis,less observed in lung and ovary.The results of immunohistochemical staining showed that the expression of AQP9 protein varied with the development of buffalo ovarian tissue,and gradually enhanced with follicle development.In testicular tissue,AQP9 protein expressed in spermatocyte and leydig cells of developmental stage testis.These results indicated that we had successfully cloned buffalo AQP9 gene sequences.The expression and its function of AQP9 in buffalo ovaries and testes might play an important role in follicle development and spermatogenesis.     

某研究堆破损元件分析判断方法

本文介绍了某研究堆堆外元件破损检查系统结构及破检流程,分析破损元件内裂变核素核释放行为以及各裂变核素的特性,提出137Cs作为堆外元件破损检查中判断元件是否破损的标准特征核素,并对该研究堆众多的堆外元件破损检查结果数据分析统计,结合理论计算认为当元件破损检查结果中137Cs活度浓度超过某一数值时可认为该元件极可能破损,利用这一结果很好筛查了该研究堆的破损元件。     

两面针水提物对酵母朊病毒作用研究

为研究两面针水提物的抗朊病毒作用,借助酵母朊病毒[PSI+]表型系统分析两面针水提物对[PSI+]表型的治疗作用,引入半变性琼脂糖凝胶电泳技术在蛋白水平分析两面针水提物对酵母朊病毒的治疗效果.结果显示,两面针水提物浓度为125 mg/mL时,作用酵母朊病毒[PSI+]细胞5d的治愈率为90.2％;并且两面针水提物浓度在25 mg/mL～125 mg/mL范围内,药物剂量与酵母朊病毒[PSI+]表型治疗效果呈现较好正相关性.蛋白水平试验表明两面针水提物作用酵母朊病毒[PSI+]细胞5d后红色菌落的朊病毒聚集体大小与[psi-]相似.     

行走青海湖

湟源的清晨是如此的清冽、透明。我们吃罢早餐,乘着大巴从湟源县宾馆出发,目标是神往已久的圣湖青海湖。以省命名的湖泊在我国应该当属青海湖了。青海湖位于青海省东北部的青海盆地内,它是中国最大的内陆湖泊,也是国内最大的咸水湖,古称西海,又称     

蒙古包大宁荒漠草场初级生产力动态研究

本文通过对蒙古包大宁十珍珠十红砂草场初级生产力动态监测的研究，摸清蒙古包大宁草场的月、季、年间的生产力变化动态规律。并探求水、热因子对草场生产力的影响，以便为合理利用草原提供科学依据。     

深化认识落实责任努力推动畜牧兽医工作上新水平——省农业厅厅长谢悦新在全省畜牧兽医工作会议上的讲话

当前,全省上下正在深入贯彻落实省委十届三次全会精神。为了推动全省畜牧兽医工作上新水平,促进全省畜牧业发展再上新台阶,进一步发挥畜牧业对于农业增效、农民增收和确保市场有效供给的作用,厅党组决定召开全省畜牧兽医工作会议：县以上畜牧兽医行政管理部门和动物防疫监督机构主要负责同志一起参加全省畜牧兽医工作会议,是改革开放以来我省畜牧业系统的第一次,     

小城记忆：五通桥的河光水影

五通桥是个居于盆地边缘的川南小城。当年徐悲鸿盘桓于此时,曾把它称为。中国的君士坦丁堡”,“群山环水,巨榕簇簇,如岗如峦,列于水旁。倒影沉沉．有若图绘。”他的这段话道出了小城的神貌,而水则是这个小城的魂,缓慢而悠然,不时打着漩儿,时而清澈时而浑浊的河流,成就了它的诗情画意。     

一例水牛子宫脱出的诊治报告

子宫脱出,是子宫、子宫角、子宫体、子宫颈及阴道等翻转突垂于阴门之外。若子宫角的一部分翻转突入阴道内,称不完全的子宫内翻;若全部子宫角翻转突入阴道内,称完全的子宫内翻。水牛子宫脱出主要是由于怀孕期运动不足、胎水过多、胎儿过大、多胎怀孕子宫过度伸张、宫肌弛缓等因素而发病,多在分娩后12h内子宫颈尚未缩小和胎衣未排出时发生。1发病情况发病牛为册亨县者楼镇红旗村陈某家的水牛,该牛青灰色毛,年龄约6岁,体重约500kg。于2010年8月11日晚产出一头母犊,产犊后该牛卧地不起,不停地努责,导致子宫