天敌昆虫低温贮藏研究进展

在农业生产中由于化学农药的长期不合理使用,导致"3R"问题持续存在和人类赖以生存的环境日益恶化,生物防治产品及技术在有害生物综合治理中的作用越发重要。天敌昆虫的生长发育及存活期往往很短,探索其储存技术是天敌昆虫规模化生产中的关键性环节。低温贮藏可以延长天敌昆虫的寿命,进而可以为生物防治提供充足、稳定的天敌产品。因此,天敌昆虫低温贮藏一直是生物防治领域研究的热点。本文围绕天敌昆虫低温贮藏这一环节,就低温诱导天敌昆虫滞育虫态、冷驯化对昆虫低温贮藏效果的影响、低温贮藏对天敌昆虫适合度(寿命、羽化率、性比、个体尺寸大小、飞行扩散能力等)的影响等方面进行综述,以期为天敌昆虫产品的规模化生产及应用技术提供参考。     

硅藻土杀虫剂处理后储粮害虫成虫体重减轻与死亡

研究了硅藻土杀虫剂“普粮泰”对米象、谷蠹、玉米象和锯谷盗成虫体重的影响。在30±1℃、65%±1%RH条件下,试虫分别在0.5~8 g/m2的粉剂药膜上处理1天,它们(顺序同上)的平均体重分别减少了0.1、0.4、0.2和0.2 mg/头,在相同条件下培养7天后,它们的平均死亡率分别为52%、100%、97%和100%。在25±1℃、65%±1%RH条件下,经过1 g/m2粉剂药膜处理锯谷盗1天后,在原条件下不提供食物保持3天,平均每头锯谷盗体重比对照减少了0.2~0.3 mg/头,平均死亡率达到76%。由此可见,硅藻土杀虫剂处理的储粮害虫成虫体重明显减少(P<0.05),死亡率很高,说明硅藻土杀虫剂可能破坏了试虫体壁保持体内水分的蜡质层,从而引起锯谷盗失水干燥而死。     

Genetic mapping and utilization of quantitative trichome-mediated insect resistance in potato

Introgression of trichome-mediated insect resistance from the wild speciesSolanum berthaultii has become a major focus of the potato improvement program at Cornell University during the past twelve years. Several quantitative characters are involved in this resistance which is effective against a wide range of pest types. Correlative biochemical assays have been developed to assay specific components of the resistance, and the effects of the resistance on the target pests have been studied. Quantitative laboratory assays and specific measurements of insect behavior and biology have increased the precision of selection and enable the investigation of the genetic control of the resistance.We are currently using restriction fragment length polymorphisms (RFLPs) for genetic mapping of factors controlling the trichome traits fromS. berthaultii. Backcrosses to both the wild and the cultivated species parents have been evaluated for phenotypes contributing to the resistance mechanism, including trichome density, sucrose ester and polyphenol oxidase production by the trichomes, and the enzymatic browning reaction responsible for insect entrapment. Genetic maps are being developed for these progenies, using RFLP markers previously mapped in potato. Field and greenhouse trials under insect infestations are also being conducted with the mapping progeny. Our goal is to locate genes responsible for quantitative insect resistance by correlating RFLP variation at mapped loci with the trichome phenotypes and insect resistance. Genetic markers for these traits will be useful in transfer of the effective wild chromosomal segments into and among tetraploid potatoes, and for a better understanding of the resistance mechanism.     

Laboratory evaluation of the insect growth regulator lufenuron againstHelicoverpa armigera on cotton

An insect growth regulator (IGR), lufenuron (Match 5EC), was tested for its toxicity toHelicoverpa armigera on cotton. Potency of the IGR against the larval stage of the pest was demonstrated with respect to larval instars; the LC₉₀ values of 1st, 2nd, 3rd, 4th and 5th instar larvae were 5.63, 7.89, 8.03, 11.39 and 14.76 mg a.i.l ⁻¹, respectively. However, different larval instars did not differ significantly with respect to LC₅₀ and LC₁₀. IGR-treated larvae had swollen heads and were significantly smaller (1.5–2.3 mm) than the untreated control (2.9 mm). Larval weight was significantly reduced from 190 mg in the control to 50–70 mg in the lufenuron treatment. IGR treatment in the larval stage significantly affected both pupal length and pupal weight. Pupal duration of the test insect was significantly extended by IGR treatment. Pupal deformities, including an inability to shed the last larval skin and formation of larval-pupal intermediates, occurred following treatment. A significant reduction in adult emergence was recorded. In addition, abnormalities in the form of development of cavities in the forewings of adult were evident. A significant decline in fecundity was noted in the studies. http://www.phytoparasitica.org posting Feb. 3, 2003.     

黄花蒿粗提物对几种害虫拒食性的初步研究

以黄花镐为原料进行浸提，经生物活性测定，结果表明黄花蒿Artenisia annua L.粗提物对供试的6种害虫均具有拒食性。其中对黑翅土白蚁Odontotermes formosanus Shiraki、赤拟谷盗Thibolium castaneum Herbst、谷蠹Rhizopertha dominica Fabricius拒食性极强，对棉蚜Aphis gossypii Glover、棉红蜘蛛Tetranychus urticae Koch及豇豆荚螟Etiella zinckenella Treitschke 也具有较强的巨食性。使用黄花蒿粗提物时，以稀释500倍和800倍效果最好，处理与对照之间有极显著差异。     

丙硫磷控制稻瘟病的作用方式及应用技术

经１９９３－１９９５年生物测定和田间多点试验示范发现；１．丙硫磷对稻瘟菌孢子萌发和附着胞形成有显著的抑制活性，ＥＣ５０为１０３．３ｍｈ／Ｌ，５００ｍｇ／Ｌ的抑菌率达９６．２％；２．丙硫磷１０００ｍｇ／Ｌ对水稻苗叶瘟和穗瘟的预防效果分别为７９．４％和９０．７％，治疗效果分别为３９．３％和４１．８％；３丙硫磷６００－９００ｇ／ｈｍ＾２防治双季稻和瓜后稻穗瘟病的田间小区效果分别为７０．８％－８０．２％和     

观赏桃园昆虫群落结构及多样性的研究

对观赏桃园昆虫群落的结构及多样性研究表明:观赏桃园昆虫有61种,隶属于11目44科。物种数随季节变化而变化,主要优势种为桃小绿叶蝉(Empoasca flavescensFabr.)和山楂叶螨(Tetranychus viennesisZacher)。在不同时间其优势度、多样性、均匀性等也不断变化。根据群落的相异系数对观赏桃园6~9月的昆虫群落进行分析表明,9月上旬与9月中旬的昆虫群落最为相似,其次是7月下旬与8月中旬。     

南方菜地辣椒主要病虫害综合防治技术

本文较为系统地总结了我国南方及长江中下游地区,辣椒秋延晚栽培的主要病虫害综合防治技术及其措施,坚持以农业防治和生物防治为主,化学防治为辅,生态调控与物理防治相结合,在无公害辣椒大面积生产中逐步实现病虫害的可持续治理。     

高纯氮气对储粮害虫致死效果的研究

本研究在30±1 ℃,相对湿度75%±5%的高纯氮气环境中对几种主要储粮害虫进行生物测定并检测结果.玉米象、米象、谷蠹、赤拟谷盗(中山品系)、杂拟谷盗、赤拟谷盗(益阳品系)、嗜卷书虱和锯谷盗的LT50值由大到小排列顺序为34.0 h、32.5 h、32.5 h、13.9 h、13.4 h、12.5 h、5.4 h和5.1 h.不同试虫对高纯氮气环境忍耐能力差异显著.     

BP神经网络在储粮害虫分类中的应用研究

综合利用计算机视觉技术和BP神经网络技术,实现对粮仓害虫的无损检测.通过对粮仓害虫图像的CCD图像预处理,获取了几何特征和不变矩等15个特征参数,并通过优化选取其中七个参数输入神经网络进行训练.仿真结果表明训练网络对粮仓四类常见害虫的识别率达到了85%,得到了较好的识别结果.