全文获取类型
收费全文 | 18230篇 |
免费 | 1236篇 |
国内免费 | 1272篇 |
专业分类
林业 | 912篇 |
农学 | 1835篇 |
基础科学 | 190篇 |
906篇 | |
综合类 | 6139篇 |
农作物 | 1421篇 |
水产渔业 | 1071篇 |
畜牧兽医 | 5239篇 |
园艺 | 980篇 |
植物保护 | 2045篇 |
出版年
2024年 | 96篇 |
2023年 | 311篇 |
2022年 | 589篇 |
2021年 | 662篇 |
2020年 | 716篇 |
2019年 | 799篇 |
2018年 | 555篇 |
2017年 | 735篇 |
2016年 | 750篇 |
2015年 | 730篇 |
2014年 | 988篇 |
2013年 | 1101篇 |
2012年 | 1232篇 |
2011年 | 1189篇 |
2010年 | 1038篇 |
2009年 | 1006篇 |
2008年 | 927篇 |
2007年 | 967篇 |
2006年 | 832篇 |
2005年 | 686篇 |
2004年 | 598篇 |
2003年 | 496篇 |
2002年 | 382篇 |
2001年 | 425篇 |
2000年 | 431篇 |
1999年 | 393篇 |
1998年 | 280篇 |
1997年 | 229篇 |
1996年 | 206篇 |
1995年 | 199篇 |
1994年 | 186篇 |
1993年 | 170篇 |
1992年 | 191篇 |
1991年 | 129篇 |
1990年 | 104篇 |
1989年 | 95篇 |
1988年 | 61篇 |
1987年 | 54篇 |
1986年 | 32篇 |
1985年 | 22篇 |
1984年 | 13篇 |
1983年 | 16篇 |
1982年 | 21篇 |
1981年 | 12篇 |
1980年 | 25篇 |
1979年 | 19篇 |
1978年 | 9篇 |
1976年 | 8篇 |
1956年 | 5篇 |
1955年 | 6篇 |
排序方式: 共有10000条查询结果,搜索用时 62 毫秒
1.
The present study was designed to investigate the potential application of native (N) and recombinant (truncated modified [tmFliC] and full-length [flFliC]) flagellin proteins along with inactivated Newcastle disease virus (NDV). Fifty six SPF chickens were immunized twice with PBS (control), inactivated NDV (Ag), inactivated NDV/flFliC (AgF), inactivated NDV/tmFliC (AgT), inactivated NDV/N (AgN), commercial vaccine containing Montanide (Vac) and Vac/N (VacN), with a two-week interval. Blood was collected weekly and spleens were harvested after chickens were sacrificed. Interleukin-6 (IL-6) and tumor necrotic factor-α (TNF-α) gene expression in peripheral blood mononuclear cells were analyzed by Real-Time PCR. Antibody response was assessed by haemagglutination inhibition (HI). Cellular activity was quantified by MTT assay. Results showed that the most IL-6 and TNF-α gene expression was observed in AgF group (P < 0.01). The lowest gene expression among vaccinated groups was observed in Ag group for IL-6 and Ag and Vac group for TNF-α. The highest HI titer was observed in Vac, VacN, AgF and AgT groups. The AgF group showed the highest cellular activity (P < 0.01). In conclusion, flagellin-adjuvanted groups showed a pro-inflammatory effect and acted similarly to or better than the Vac group. Hence, flagellin can be proposed as a potential adjuvant for ND vaccine. 相似文献
2.
3.
AIM: To investigate the role of zerumbone (ZER) in 1-methyl-4-phenylpyridinium (MPP+)-induced cytotoxicity of human neuroblastoma SH-SY5Y cells. METHODS: Human neuroblastoma SH-SY5Y cells were cultured in vitro and the protective effect of ZER against MPP+-induced cytotoxicity was measured by CCK-8 assay. Flow cytometry was used to determine the apoptosis and reactive oxygen species (ROS). The expression of Parkinson disease protein 7 (PARK7) was knocked-down by using PARK7-specific short hairpin RNA (shRNA). The protein levels of PARK7, nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot. RESULTS: MMP+ remarkably reduced the cell viability in a dose-dependent and time-dependent manner. The SH-SY5Y cell injury model was established by treatment with MPP+ at 600 μmol/L for 24 h. ZER up-regulated the protein levels of PARK7 and Nrf2 (P<0.05), alleviated apoptosis (P<0.05), and reduced ROS production (P<0.05) in the SH-SY5Y cell injury model. Meanwhile, N-acetyl-L-cysteine (NAC) had the similar functions. Moreover, significant reductions in the protein levels of Nrf2 and HO-1 (P<0.05), and obvious increases in apoptosis (P<0.05) and ROS level (P<0.05) were demonstrated in PARK7-knockdown cells. CONCLUSION: ZER protects SH-SY5Y cells against MPP+-induced cytotoxi-city, which may be related to activation of PARK7/Nrf2/HO-1 pathway, and subsequent attenuation of oxidative stress and apoptosis. 相似文献
4.
5.
为了研究不同小麦品种萌发期的耐盐性,为小麦萌发期耐盐性鉴定提供快速、准确的鉴定方法和理论依据。本研究以40个冬小麦品种为试验材料,通过主成分分析法(PCA)与神经网络自组织映射(SOM)聚类分析法,研究盐胁迫对40个小麦品种萌发期各项形态指标的影响。主成分分析法结果表明,发芽势、发芽率、最长根长、第一片叶长及胚芽鞘长在PCA下载荷量最大;根据SOM聚类分析结果,‘德抗961’等4个小麦品种属于高度耐盐品种,‘济麦22’等14个小麦品种属于耐盐性品种,‘济麦20’等16个品种属于中等耐盐性品种,‘鲁麦21’等6个小麦品种属于盐敏感性品种。发芽势、发芽率、最长根长、第一片叶长、胚芽鞘长能够作为萌发期小麦耐盐性筛选的主要评价指标,并且结合主成分、SOM聚类分析方法进行小麦萌发期耐盐性鉴定更为科学、方便。 相似文献
6.
黄瓜病虫害种类多、危害大,传统用药方式严重影响产品质量和口感。为了保证直供上海的黄瓜达到无公害要求,对生产基地采取农业防治、生态控制、生物防治和物理防治相结合的绿色防控措施,化学农药使用量减少30%,综合防治效果在85%以上,有效减少了防治次数,降低了农产品的农药残留,667 m~2增产600 kg,增值600元左右。收到了较好的经济、生态及社会效益。 相似文献
7.
以从海滨雀稗中分离得到的内生肠杆菌为材料,采用种子萌发与盆栽试验两种方式研究植物内生细菌对狗牙根耐盐性的影响。通过对狗牙根种子和幼苗接种单一内生细菌和混合内生细菌,并测定种子发芽率、苗长、根长、生物量等生长指标与叶绿素含量、相对含水量、相对电导率和叶片中钠离子、钾离子的含量综合评价植物内生细菌对狗牙根耐盐能力的调控。结果表明,在150 mmol·L-1盐胁迫下处理14 d后,接种混合菌液B3014比接种单一菌液B30更能提升狗牙根种子的萌发率、对盐胁迫下胚根及胚芽长度的增长也较显著(P<0.05)。在成坪期,与空白对照相比,接种内生细菌可以提升200 mmol·L-1盐胁迫下狗牙根的成坪质量,增加地上、地下生物量的积累、苗长与根长生长量,并提升植株叶绿素含量与叶片相对含水量,降低细胞膜破损程度。在接种内生细菌处理中,接种混合菌液B3014可以使狗牙根在盐胁迫下受到的损伤显著低于(P<0.05)接种单一菌液B30。与此同时,接种混合菌液B3014对狗牙根叶片中钠离子含量降低程度高于接种单一菌液B30,同样,接种混合菌液B3014也能更好地积累盐胁迫下狗牙根叶片内钾离子含量,增加盐胁迫下狗牙根的耐盐能力。因此,接种混合菌液B3014对盐胁迫下狗牙根种子的萌发和成坪后的耐盐能力的提升高于接种单一菌液B30。探讨植物内生细菌对狗牙根耐盐能力的调控,为提高草坪草在盐碱环境中的应用提供了新思路。 相似文献
8.
9.
施用锌肥和遮阴互作对花生生长发育、抗病性及产量的影响 总被引:1,自引:0,他引:1
采用大田裂区试验,研究了施用锌肥和不同遮阴程度互作对花生生长发育、抗病性及产量的影响。结果表明,与不施锌肥相比,施用锌肥能提高花生不同部位锌含量、增加叶片SPAD值,提高花生叶片中可溶性糖、蛋白质和生长素含量,减少花生病害的发生,平均降低7.1个百分点,花生产量平均增加19.4%。相同施锌水平下,随着遮阴程度的增加,花生不同部位锌的含量和不同生育期叶片SPAD值以及花生叶片中可溶性糖、蛋白质和生长素含量呈增加趋势,花生的发病率比不遮阴对照增加4.8、10.2个百分点,花生产量平均降低16.5%、10.0%。在30%、70%的遮阴条件下,施用锌肥的花生产量比不施锌的分别提高21.1%、25.0%。本试验条件下,施用七水硫酸锌30 kg/hm2,使花生具有较强的抗低温寡照能力及抗病性能,增产显著,可在花生产区推广应用。 相似文献
10.
A disease causing the decline of papaya (Carica papaya) plants was noticed in the Jordan Valley in 1982. The disease caused severe yellowing of the upper leaves and dieback of the apex. The disease was not transmissible mechanically and continued efforts to associate viruses and viroids have so far been unsuccessful. Epidemiological observations suggested that the disease is airborne and probably caused by a mollicute. In order to test this hypothesis, plots were covered by an insect-proof net and with white nets providing 15, 30 and 50% shade. The 30%, 50% and insect-proof nettings provided complete protection, and the 15% netting reduced disease incidence to <2%, compared with>37% in the uncovered control plot. Spraying plants at weekly intervals with a commercial whitewash solution was also found to be effective in reducing disease incidence. The advantages of using a range of netting field treatments as a simple means for obtaining information on the epidemiological nature of a new disease are described. 相似文献