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通过对白屈菜低温应答过程的转录组分析发现膜脂不饱和化相关基因的表达在一定过程中发生变化,脂肪酸去饱和酶基因FAD2在随温度的变化趋势为正"V"型,且表达量变化显著。利用NCBI等在线软件对序列进行相关生物学信息分析,并对白屈菜FAD家族成员FAD2基因的完整开放阅读框(ORF)进行克隆,并命名为CmFAD2。选用克隆载体pMD-19-T,转化大肠杆菌DH5α,测序验证序列正确性及完整性。将目的基因与植物表达载体pRI-201-AN连接构建重组DNA pRI-201-AN-Cm FAD2,电击法转化农杆菌LBA4404,利用菌液PCR法验证成功。该基因可作为药用植物抗寒品种创制的候选基因。 相似文献
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Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens
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ZENG Xian-ying CHEN Xiao-han MA Shu-jie WU Jiao-jiao BAO Hong-mei PAN Shu-xin LIU Yan-jing DENG Guo-hua SHI Jian-zhong CHEN Pu-cheng JIANG Yong-ping LI Yan-bing HU Jing-lei LU Tong MAO Sheng-gang GUO Xing-fu LIU Jing-li TIAN Guo-bin CHEN Hua-lan 《农业科学学报》2020,19(9):2294-2300
We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5 N6)(DK/GZ/S4184/17)(a clade 2.3.4.4 d virus), A/chicken/Liaoning/SD007/2017(H5 N1)(CK/LN/SD007/17)(a clade 2.3.2.1 d virus), and A/chicken/Guangxi/SD098/2017(H7 N9)(CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7 N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7 N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control. 相似文献
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D. Bilalis P. Efthimiadis & G. Karagiannis 《Journal of Agronomy and Crop Science》2001,187(2):121-126
The effect of three herbicides, diclofop-methyl (DM), imazamethabenz-methyl (IM) and fenoxaprop-ethyl (FE), was investigated at the recommended rate (×) and double the recommended rate (2×) in a durum wheat crop. FE had the smallest effect on root system characteristics, while IM had the greatest effect. For all herbicides, the double rate treatment gave a greater statistical reduction than the single rate for root system characteristics. FE had the smallest effect on the activity of aerobic microorganisms, while DM gave the greatest reduction, 10 days after application, and all three herbicides reduced earthworm abundance. DM gave the smallest reduction in the number of tillers and in plant height, while the double rate of IM gave the greatest reduction. For all three herbicides, double the recommended rate resulted in a significant reduction in the weight of 1000 seeds, while no statistically significant deviations were noted at the recommended rate. Finally, the use of all three herbicides resulted in significantly lower statistical yields compared to the sample (control plot), although there were no significant statistical differences among them, for either recommended or double recommended rates. 相似文献
5.
依据干旱胁迫下数种植物幼苗体内游离脯氨酸、叶气孔、叶水量的变化,剖析了植物与环境因子、气孔因子、氧气因子、酶因子及能量因子之间的相互依存关系,提出了PRO积累系统(PAS)。认为水分胁迫下植物体内PRO的大量积累是氧化受抑和合成受激共同调控的结果。氧气最有可能为直接触发因子。 相似文献
6.
J. Müller 《Journal of Agronomy and Crop Science》1993,171(4):217-235
Dry Matter Production, CO2 Exchange, Carbohydrate and Nitrogen Content of Winter Wheat at Elevated CO2 Concentration and Drought Stress
Methods of mathematical modelling and simulation are being used to an increasing degree in estimating the effects of rising atmospheric CO2 concentration and changing climatic conditions on agricultural ecosystems. In this context, detailed knowledge is required about the possible effects on crop growth and physiological processes. To this aim, the influence of an elevated CO2 concentration and of drought stress on dry matter production, CO2 exchange, and on carbohydrate and nitrogen content was studied in two winter wheat varieties from shooting to milk ripeness. Elevated CO2 concentration leads to a compensation of drought stress and at optimal water supply to an increase of vegetative dry matter and of yield to the fourfold value. This effects were caused by enhanced growth of secondary tillers which were reduced in plants cultivated at atmospheric CO2 concentration. Analogous effects in the development of ear organs were influenced additionally by competitive interactions between the developing organs. The content and the mass of ethanol soluble carbohydrates in leaves and stems were increased after the CO2 treatment and exhausted more completely during the grain filling period after drought stress. Plants cultivated from shooting to milk ripeness at elevated CO2 concentration showed a reduced response of net photosynthesis rate to increasing CO2 concentration by comparison with untreated plants. The rate of dark respiration was increased in this plants. 相似文献
Methods of mathematical modelling and simulation are being used to an increasing degree in estimating the effects of rising atmospheric CO
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以苯—乙醇为夹带剂,研究超临界CO2流体不同处理压力、温度、时间对福建产杉木、马尾松、檫木木材渗透性的影响。结果表明,在35 MPa内,处理压力的增大有利于3种木材渗透性的提高;杉木在处理温度为40℃时,渗透性的变化率达到最大,为493%,马尾松和檫木在处理温度为45℃时,渗透性的变化率达到最大,分别为479%和349%;较佳的处理时间为30 min。 相似文献
9.
1989~1990年选用陆地棉(G.hirstum L.)北农1号品种和海岛棉(G.barbadenseL.)海7124品种为材料,对棉纤维发育后期的纤维素形成过程进行了研究。结果表明:①随着棉纤维的发育 Mg~(2+),K~+-ATP 酶活性逐渐下降。②Mg~(2+),K~+-ATP 酶活性的最适 pH 值为6.5。Mg~(2+),K~+ATP 酶活性随[Mg~(2+)],[K~+]和[NADH]/[NAD~+]的增大而变大。③Mg~(2+),K~+ATP 酶活性变化与还原性糖含量变化呈显著正相关(r=0.811~*),与纤维素含量变化呈极显著负相关(r=-0.900~(**))。④Mg~(2+),K~+-ATP 酶参与了纤维素的形成过程。 相似文献
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