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21.
Chiho KANEKO Yasuyuki KANEKO Putu Eka SUDARYATMA Hirohisa MEKATA Yumi KIRINO Ryoji YAMAGUCHI Tamaki OKABAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):680
We isolated two pseudorabies virus (PRV) isolates (designated OT-1 and OT-2) from two hunting dogs exhibiting neurological manifestations after eating the flesh of wild boar hunted in Oita prefecture, Kyushu Island, Japan. The isolates corresponded to a previously reported PRV (MY-1 strain) isolated from a hunting dog in neighboring Miyazaki prefecture, and it clustered into genotype II based on the glycoprotein C sequence. Our results suggest that this common PRV strain may have been maintained in wild boars on Kyushu Island even though domestic pigs in this area have attained an Aujeszky’s disease-free status. 相似文献
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Black-backed jackals (Canis mesomelas) are an abundant mesopredator on farmlands (croplands and rangelands) across South Africa. Given their adaptability in a changing anthropogenic landscape, knowledge of spatial movements can provide important ecological information on the species. We captured, collared and monitored five black-backed jackals in the Midlands of KwaZulu-Natal to determine their home range and habitat use in farmlands. The 95% fixed kernel (95% FK) home range sizes were large, showing a marked seasonal variation in movement. One adult male dispersed 150?km over two seasons (winter and spring) and thereafter settled into a home range that it maintained. Transient home ranges of males and juveniles were larger than resident home ranges. The mean resident home ranges (95% FK) across seasons for adult males, adult female and juvenile males were 11.4 ± 4.3 km2, 5.6 ± 0.36?km2 and 2.15 ± 0.45?km2, respectively. The mean transient home ranges (95% FK) of all the seasons for adult and juvenile males were 1 181.93 ± 575.81 km2 and 104.27 ± 35.64?km2, respectively. Adult jackal preferred croplands in spring, summer and autumn but avoided croplands in winter. Variable habitat use and large home ranges in our study confirmed the species’ ability to adapt to agricultural areas. 相似文献
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Kanae SHIOKAWA Chandika D. GAMAGE Nobuo KOIZUMI Yoshihiro SAKODA Kenta SHIMIZU Yoshimi TSUDA Kumiko YOSHIMATSU Jiro ARIKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):221-230
The applicability of the recombinant LipL32 for serodiagnosis of leptospiral infection in field rodents was
assessed in this study. An immunodominant region of LipL32 was determined by monoclonal antibodies, and then,
truncated LipL32 (tLipL32) was designed to contain the region (87–188th amino acid). The tLipL32 was compared
between two recombinant expression hosts Escherichia coli and Pichia
pastoris in ELISA. With field rat sera, tLipL32 expressed by P. pastoris
(tLipL32p) had high antigenicity without background reactions, while tLipL32 expressed by E.
coli (tLipL32e) showed high background reactions, which were reduced by pre-adsorption of sera with
E. coli. To evaluate tLipL32-ELISA, field rat sera were tentatively divided into a
Leptospira infection positive (12 sera) and a negative group (12 sera) based on the results
from flaB gene PCR of kidney samples and WB with whole Leptospira cell.
Consequently, the sensitivity of tLipL32p-ELISA for field rat sera was 83% . A similar result was obtained
from tLipL32e-ELISA with adsorbed sera, (92%). However, sensitivity of tLipL32e-ELISA using sera without an
adsorption treatment was 50%. Regardless of the expression host, tLipL32-ELISA had 100% specificity and
sensitivity in experimentally infected laboratory rats. These results suggest that recombinant LipL32
expressed by P. pastoris is more applicable for serodiagnosis in field rats due to a lack of
background reaction. 相似文献
24.
Lindsay M. Clontz Anni Yang Sarah M. Chinn Kim M. Pepin Kurt C. VerCauteren George Wittemyer Ryan S. Miller James C. Beasley 《Pest management science》2023,79(10):3819-3829
Background
Data on the movement behavior of translocated wild pigs is needed to develop appropriate response strategies for containing and eliminating new source populations following translocation events. We conducted experimental trials to compare the home range establishment and space-use metrics, including the number of days and distance traveled before becoming range residents, for wild pigs translocated with their social group and individually.Results
We found wild pigs translocated with their social group made less extensive movements away from the release location and established a stable home range ~5 days faster than those translocated individually. We also examined how habitat quality impacted the home range sizes of translocated wild pigs and found wild pigs maintained larger ranges in areas with higher proportion of low-quality habitat.Conclusion
Collectively, our findings suggest translocations of invasive wild pigs have a greater probability of establishing a viable population near the release site when habitat quality is high and when released with members of their social unit compared to individuals moved independent of their social group or to low-quality habitat. However, all wild pigs translocated in our study made extensive movements from their release location, highlighting the potential for single translocation events of either individuals or groups to have far-reaching consequences within a much broader landscape beyond the location where they are released. These results highlight the challenges associated with containing populations in areas where illegal introduction of wild pigs occurs, and the need for rapid response once releases are identified. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. 相似文献25.
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为了筛选耐盐樱桃砧木,用于天津市周边轻度盐碱地区栽培,本试验通过徒手切片的方法,研究了两种樱桃砧木在盐胁迫下的叶片解剖结构变化。结果表明:在盐胁迫下,四川樱桃的叶片增厚了3.99%,大青叶的减少了2.68%;大青叶的表皮层细胞增厚了23.6%,四川樱桃的减少了3.84%;大青叶的角质层增厚3.57%,四川樱桃的减少了18.31%;四川樱桃的栅栏组织层变化不大,大青叶的减少了16.42%;四川樱桃的海绵组织层增厚了31.96%,大青叶的增厚了21.27%;四川樱桃的栅栏组织和海绵组织层厚度的比值变小、减少了29.17%,大青叶的减少了20.80%。盐胁迫使大青叶叶片表皮细胞层变厚,角质层加厚,栅栏组织层变薄,海绵组织层加厚,整个叶片变薄。盐胁迫使四川樱桃表皮细胞层变薄,角质层变薄,海绵组织层加厚,整个叶片变厚。从盐胁迫下的2种樱桃叶片结构的变化综合分析认为大青叶的耐盐性明显高于四川樱桃。 相似文献
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