薄层扫描法测定人参降糖胶囊中AF的含量

采用薄层扫描法测定人参降糖胶囊中的精氨酸衍生物AF的含量。以正丁醇—冰醋酸—水(2∶1∶1)为展开剂,以2 g/L茚三酮乙醇溶液为显色剂,AF的测定波长λS=510 nm,参比波长λR=685 nm。测定结果表明:AF在1.00~3.50μg范围内线性关系良好,r=0.998 1。平均回收率99.77%(n=6),RSD=1.34%。     

乙肝清胶囊中金丝桃素含量的测定

[目的]建立乙肝清胶囊中金丝桃素的HPLC含量测定方法。[方法]筛选超声和加热回流提取2种方法在不同的提取溶媒下对胶囊内容物的前处理效果,采用HPLC法测定金丝桃素含量。色谱条件:色谱柱采用Phenomenex Luna C18柱(4.6 mm×250 mm,5μm),流动相为甲醇-2.5 g/L KH2PO4水溶液(95∶5,V/V),流速为1.0 ml/min,检测波长为588 nm。[结果]最优提取方法为:以甲醇为提取溶媒在90℃水浴加热回流30 min;金丝桃素在0.108~1.080μg范围内线性关系良好,r=0.999 84,平均回收率为98.8%,RSD=1.65%。[结论]所建立的乙肝清胶囊中金丝桃素HPLC含量测定方法具有良好的稳定性、重现性和准确性,可用于乙肝清胶囊的质量控制。     

循证医学在验证安体邦软胶囊中马尾松松花粉降血脂功效的应用

[目的]运用循证医学的方法,验证安体邦软胶囊中马尾松松花粉的降血脂功效,为降血脂功效研究提供方法学借鉴。[方法]选取血脂偏高的100人作为研究对象,随机分为2组,试验组与对照组各50例。[结果]服用安体邦软胶囊后甘油三酯(TG)降低的有62.16%,高密度脂蛋白胆固醇(HDL-C)增高的有56.76%,低密度脂蛋白胆固醇(LDL-C)降低的有70.27%,总胆固醇(TC)降低的有67.57%;对照组50例,服用前后无明显变化。[结论]安体邦软胶囊具有较好的降血脂功效。     

超微粉中草药添加剂对热应激蛋鸡血清中酶、pH值、无机离子、HSP70的影响

试验探讨超微粉中草药添加剂对热应激蛋鸡血清中酶、pH值、无机离子、HSP70的影响。选取105只伊萨褐蛋鸡,随机分为5组,每组21只。A组为对照组,饲喂基础日粮;B、C、D、E组在基础日粮中分别添加0.5%、1.0%、1.5%、2.0%的中草药超微粉添加剂,进行7周的饲养试验。结果表明：D、E组血清ALT比A组显著降低（P<0.05）,C、D组血清ALP较A组显著提高（P<0.05）,D、E组血清CK较A组显著下降（P<0.05）;B、C、D、E组血清pH值较A组显著降低（P<0.05）,B、C、D、E组的血清K较A组显著增高（P<0.05）,D、E组血清Ca较A组显著增高（P<0.05）,B、C、D、E组P较A组显著增高（P<0.05）;C组血清HSP70与A组有极显著差异（P<0.01）,D、E组血清HSP70比A组显著提高（P<0.05）,超微粉中草药添加剂能够明显改善蛋鸡血液有关生化指标,诱导HSP70的表达,缓解热应激。     

A cost-benefit analysis of pre- and post-lambing anthelmintic treatments to twin-bearing ewes on commercial farms in the southern North Island of New Zealand

Abstract

AIM: To conduct a cost-benefit analysis of the administration of anthelmintics to adult ewes around lambing.

METHODS: Production data from comparisons of different anthelmintic treatments with no treatment were used in a cost-benefit analysis. The data were from 14 trials (part of an experiment carried out on one farm in 1 year) conducted on sheep and beef farms (eight in 2011 and six in 2012) in the Wairarapa region of New Zealand. The cost structure involved the purchase price of products and the labour cost of administration. The four key benefits identified for the calculation of economic returns, relative to untreated ewes, were: increased value of ewes sold (culled) at weaning, additional lambs weaned related to ewe liveweight at mating, increased total weight of lamb weaned per ewe, and reduced number of ewes requiring removal of soiled wool at weaning due to a lower dag score. Commercial values for these variables as at December 2013 were used, with the measured production data, to calculate a net (NZ$) benefit for every treatment-trial combination.

RESULTS: The economic return on treating ewes around lambing with anthelmintics was highly variable and across all trials treatment resulted in a financial loss in 18/38 (47%) groups of ewes. The mean net benefit from pre-lambing administration of a controlled release capsule (CRC) containing albendazole and abamectin was 5.36 (95% CI=?2.64 to 13.35) $/ewe, but overall was not different from zero (p=0.171). A breakdown of the overall gross benefit into its various components showed that weight of lamb weaned per ewe had the largest influence (a mean benefit of $5.68/ewe), followed by ewe liveweight pre-mating ($2.45/ewe), ewe liveweight at weaning ($0.66/ewe) and reduced dag score ($0.15/ewe). Other anthelmintic treatments all showed highly variable responses amongst trials, with some negative cost-benefits. There was no significant difference between any of the treatments except a short-acting oral treatment at tail-docking had a lower net benefit than a CRC containing albendazole administered pre-lambing (p<0.05).

CONCLUSIONS: A positive financial return resulting from the anthelmintic treatment of adult ewes around lambing is neither consistent nor predictable, and is often not achieved. Given that the additional costs of accelerating the development of anthelmintic resistance were not included in these calculations, farmers need to consider carefully the merits of administering anthelmintics to ewes around lambing.     

Effect of Fufang Zhenzhu Tiaozhi capsule on serum lipids and inflammatory factors in rabbit abdominal aortic restenosis model

AIM: To investigate the effect of Fufang Zhenzhu Tiaozhi capsule (FTZ) on serum lipids and inflammatory factors in rabbits with abdorminal aortic restenosis after balloon angioplasty.METHODS: New Zealand white rabbits (n=30) were divided into 5 groups. Except blank control group, the rabbits in other groups were used to establish abdominal aortic endothelium exfoliative vascular stenosis model. After 4 weeks of high-fat diet feeding, the animals in restenosis model group and drug treatment groups underwent percutaneous balloon dilatation in the stenosis. The angiographic stenosis was analyzed by a two-dimensional quantitative coronary angiography workstation with a digital subtraction X-ray machine. Blood samples were taken during angiography and the profiles of serum lipids and cytokines were measured. The expression of nuclear factor-κB (NF-κB) in the blood vessels was determined by immunohistochemistry.RESULTS: Angiography confirmed that the rates of area stenosis and diameter stenosis were significantly decreased in treatment groups compared with restenosis model group (P<0.01). Compared with restenosis model group, the serum lipid profiles and cytokine concentrations in drug treatment groups were significantly decreased (P<0.05). Immunohistochemistry showed the expression of NF-κB in restenosis model group was significantly higher than that in blank control group and drug treatment groups (P<0.05).CONCLUSION: FTZ significantly reduces the blood lipids and inflammatory factors in abdominal aortic restenosis model, and the anti-inflammatory effect may be related to the regulation of NF-κB pathway to inhibit the production of various inflammatory factors.     

Sheared heels and the correlation to spontaneous quarter cracks

The strong association between sheared heels and a spontaneous quarter crack is hard to ignore. Although inappropriate farriery may play a role, limb conformation and the landing pattern of the horse appear to be the dominant factors causing this type of hoof capsule deformation. The importance of determining the underlying cause and implementing the appropriate farriery cannot be over emphasised when managing a quarter crack associated with a sheared heel. The repair of spontaneous quarter cracks will be of little value, and the defect will have a tendency to recur, unless the cause is identified and rectified.     

竹炭超微粉制备与性能结构初步研究

采用高能球磨法制备竹炭超微粉,并对其性能结构进行初步分析。结果表明,与原颗粒竹炭相比,经球磨后的竹炭粒径明显减小,碘吸附值增加,体积电阻率下降。利用高能球磨法可制得微米级至亚微米级的不规则多边形竹炭超微粉。     

HPLD法测定复方石淋通胶囊中绿原酸的含量

[目的]建立复方石淋通胶囊中绿原酸含量的测定方法。[方法]采用高效液相法,色谱柱为ZORBAXSB-C18（5μm,4.6mm×250.0mm）,流动相为乙腈-0.2%磷酸水溶液（8∶92,V/V）,流速为1.2ml/min,检测波长为327nm。[结果]绿原酸在0.04～0.08μg/ml范围内呈现良好线性关系,加样回收率为101.7%,RSD值为1.88%（n=6）。[结论]高效液相法测定复方石淋通胶囊中绿原酸的含量方法操作简单、准确、重复性好、精密度高、专属性强,可以作为复方石淋通胶囊的含量测定标准。