微生态制剂对葡萄抗病能力及果实品质的影响

以云南、四川主栽的4个葡萄品种为试材,在常规用药方式基础上辅以微生态制剂,测定其对葡萄酸腐病和黑曲霉病防控效果及对果实品质的影响,以期为有效防控葡萄酸腐病和黑曲霉病的发生,解决生产实践中的具体问题提供科学参考依据。结果表明:微生态制剂能提高葡萄果粒大小和百粒质量,同时增加糖酸比。在传统用药基础上使用绿康威,对葡萄酸腐病和黑曲霉病的防效最佳,分别达52.3%和42.4%;在传统用药基础上使用绿地康3号,其防效为31.3%和21.9%,均表现出良好的防效;前期使用化学药剂,后期单独使用微生态制剂对酸腐病的防效不太明显。     

Inhibitory Effects of AURKB Gene on Apoptosis and Cancer Cell Growth in HCT 116 Cells

[Objectives]To explore the inhibitory effect of AURKB gene in apoptosis and cancer cell growth in HCT 116 cells.[Methods]The in vitro cytology studies were carr...     

白屈菜CmFAD2基因的克隆及植物转化载体构建

通过对白屈菜低温应答过程的转录组分析发现膜脂不饱和化相关基因的表达在一定过程中发生变化,脂肪酸去饱和酶基因FAD2在随温度的变化趋势为正"V"型,且表达量变化显著。利用NCBI等在线软件对序列进行相关生物学信息分析,并对白屈菜FAD家族成员FAD2基因的完整开放阅读框(ORF)进行克隆,并命名为CmFAD2。选用克隆载体pMD-19-T,转化大肠杆菌DH5α,测序验证序列正确性及完整性。将目的基因与植物表达载体pRI-201-AN连接构建重组DNA pRI-201-AN-Cm FAD2,电击法转化农杆菌LBA4404,利用菌液PCR法验证成功。该基因可作为药用植物抗寒品种创制的候选基因。     

Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens

We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5 N6)(DK/GZ/S4184/17)(a clade 2.3.4.4 d virus), A/chicken/Liaoning/SD007/2017(H5 N1)(CK/LN/SD007/17)(a clade 2.3.2.1 d virus), and A/chicken/Guangxi/SD098/2017(H7 N9)(CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7 N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7 N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.     

Phenological and physiological evaluation of first and second cropping periods of sorghum and maize crops

Environmental conditions influence phenology and physiological processes of plants. It is common for maize and sorghum to be sown at two different periods: the first cropping (spring/summer) and the second cropping (autumn/winter). The phenological cycle of these crops varies greatly according to the planting season, and it is necessary to characterize the growth and development to facilitate the selection of the species best adapted to the environment. The aim of this study was to characterize phenological phases and physiological parameters in sorghum and maize plants as a function of environmental conditions from the first cropping and second cropping periods. Two parallel experiments were conducted with both crops. The phenological characterization was based on growth analyses (plant height, leaf area and photoassimilate partitioning) and gas exchange evaluations (net assimilation rate, stomatal conductance, transpiration and water-use efficiency). It was found that the vegetative stage (VS) for sorghum and maize plants was 7 and 21 days, respectively, longer when cultivated during the second cropping. In the first cropping, the plants were taller than in the second cropping, regardless of the crop. The stomatal conductance of sorghum plants fluctuated in the second cropping during the development period, while maize plants showed decreasing linear behaviour. Water-use efficiency in sorghum plants was higher during the second cropping compared with the first cropping. In maize plants, in the second cropping, the water-use efficiency showed a slight variation in relation to the first cropping. It was concluded that the environmental conditions as degree-days, temperature, photoperiod and pluvial precipitation influence the phenology and physiology of both crops during the first and the second cropping periods, specifically cycle duration, plant height, leaf area, net assimilation rate, stomatal conductance and water-use efficiency, indicating that both crops respond differentially to environmental changes during the growing season.     

The effect of feeding a novel multistrain yeast fraction on European seabass (Dicentrachus labrax) intestinal health and growth performance

Fish were fed a single‐strain yeast fraction (SsYF; 2 g/kg) or a multistrain yeast fraction (MsYF; 0.8 g/kg) for 10 weeks. The results demonstrated significant (p ≤ 0.03) elevations in weight gain, specific growth rate, protein efficiency ratio, and feed conversion ratio in fish fed the yeast fraction‐supplemented diets. In the distal intestine, a significant elevation in microvilli density was observed after 5 and 10 weeks of dietary supplementation with MsYF and SsYF, respectively, compared to control fed fish (p < 0.001). A significant elevation (p = 0.02) in the perimeter ratio was observed in fish fed diets supplemented with the yeast fractions. After 10 weeks of feeding on the experimental diets, Rt‐qPCR demonstrated a significant downregulation (p < 0.05) in the stress response genes, heat‐shock protein 70 (hsp70) and proliferating cell nuclear antigen (pcna), in fish fed diets supplemented with the yeast fractions. Significant (p < 0.05) elevations in interleukin 1‐beta (il1β) and interleukin‐10 (il10) gene expression were observed in fish fed diets supplemented with the MsYF compared to the other dietary groups. These findings suggest that feeding an MsYF specifically at a lower incorporation rate < 1 g/kg, compared to a commercial SsYF at 2 g/kg, is effective in improving the intestinal health status and growth performance of European seabass.     

基于森林健康理念的森林康养产业发展研究

阐述了森林健康理念和森林康养的基本概念,介绍了森林健康理念和森林康养的发展现状,分析了森林健康与森林康养产业之间的关系。结果表明:森林康养产业的理论基础就是基于森林健康理念继承发展来的,必然会促进森林健康产业的发展。运用森林健康理念指导森林康养产业发展是森林康养产业可持续发展的有效途径,森林健康理念在中国会不断完善和发展,也更有利于中国森林康养产业的发展。展望康养产业,会形成一种新的经济形态,成为国民福祉。     

不拘于形，不役于物——欧阳修的园林活动与园林书写

欧阳修自幼与园林结缘，仕宦四十年，所到之处植花造园，笔耕不辍，留下数座园林景观、二十多篇园亭记及百余首园林诗词，撰写了现存最早的牡丹专著《洛阳牡丹记》，为后人研究园林提供了珍贵的史料。欧阳修的园林活 动和园林书写与其宦海沉浮、文学创作紧密相关。欧阳修热爱但不耽溺于园林，其崇尚自然、开放自适，不拘于形，不役于物的园林观，拓宽了园林审美的视域，启发了后人对“物”的审美思考，为园林理论注入了新鲜血液。欧阳修进一步打开了园林的大门，推动了园林的开放，为园林的繁荣与普及做出贡献。     

耐盐抗旱优质高产棉花新品种‘衡棉1670’的选育研究

抗逆优质丰产棉花新品种的培育及应用是当前棉花生产节水提质增效的关键。‘衡棉1670’是河北省农林科学院旱作农业研究所以中早熟陆地棉品种资源材料‘衡棉210’为母本,以‘衡棉4号’为父本杂交,后代多年南繁北育,经旱棚盐池鉴定选择,在枯黄萎病混生重病地、不防治棉铃虫的高压胁迫下,经过多年连续定向选择育成。其突出表现为抗枯黄萎病、丰产优质、耐盐抗旱节水,适宜在河北省及黄河流域同类生态春播棉区推广种植,2019年8月通过河北省农作物品种审定委员会审定（审定编号为冀审棉20190013）。     

护林防火技术在森林资源保护中的应用

合理运用护林防火技术可以提升森林资源保护工作的整体质量,推动林业不断发展。本文分析了森林火灾发生的原因,总结了护林防火技术,提出了相关应用对策,以期为减少森林火灾的发生和保护森林资源提供参考。