猪繁殖与呼吸综合征病毒实验感染SPF猪外周血单核细胞和肺泡巨噬细胞早期凋亡细胞的观察

采用Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ／ＰＩ双染色法,用流式细胞仪检测了猪繁殖与呼吸综合征病毒（ＰＲＲＳＶ）实验感染ＳＰＦ猪不同时期外周血单核细胞和肺泡巨噬细胞感染Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ＾＋／ＰＩ＾－细胞群（早期凋亡细胞群）。结果显示,ＰＲＲＳＶ感染猪外周血单核细胞和肺泡巨噬细胞Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ＾＋／ＰＩ＾－细胞群的表达率均明显高于正常对照猪,感染后２４ｈ表达率达最高值。     

试论草坪主动除草

根据1999－202年在50多个高尔夫球场草坪防除杂草的实践，基于土壤中杂草种子库、杂草与草坪竞争的生态学原理，提出了草坪主动除草的理论。包含控杂草于危害前，树草坪竞争优势，治理杂草种子库，预防潜在的杂草问题。阐述了如何取得杂草防除、保持杂草防除的主动权以及如何制定主动防除草坪杂草的方案。     

Comparison of 3 techniques for ureteroneocystostomy in cats

OBJECTIVE: To compare 3 techniques for ureteroneocystostomy in cats. STUDY DESIGN: Experimental surgical study. ANIMALS: Fifteen adult cats. METHODS: Cats (15) had ureteroneocystostomy with ureteronephrectomy of the contralateral kidney: 5 cats had an intravesical mucosal apposition technique (modified Leadbetter-Politano; intravesical-MA group), 5 cats had extravesical ureteroneocystostomy (modified Lich Gregoir) using a simple continuous suture pattern (extravesical-SC group) and 5 cats had an extravesical technique using a simple interrupted suture pattern (extravesical-SI group). Renal function was evaluated by measuring serum creatinine concentration. Ultrasonographic assessment of the kidney and ureteroneocystostomy site was performed the day after surgery, twice weekly for 3 weeks and once weekly for the remainder of the study. Cats were euthanatized 50 days after surgery. The kidney and ureter removed at surgery, the remaining kidney, ureter, ureteroneocystostomy site, and bladder were examined histologically. RESULTS: Two extravesical-SC cats were euthanatized because of azotemia and uroabdomen, and 1 died acutely at day 4 for unknown reasons. In the intravesical-MA and extravesical-SI cats, the serum creatinine concentration increased after surgery, peaking at a mean (+/-SD) of 9.4+/-2.4 mg/dL and 4.9+/-3.3 mg/dL on day 3, and decreasing to 3.4+/-5.7 mg/dL and 1.5+/-0.4 mg/dL on day 7, respectively. The extravesical-SI technique was associated with consistently lower serum creatinine concentrations for the first week after surgery compared with the other techniques. The mean serum creatinine concentration was within the reference range in cats in the intravesical-MA and extravesical-SI groups by days 10 and 5, respectively. Renal pelvic dilatation occurred in all cats but resolved more rapidly in cats after extravesical techniques. There was no significant difference in serum creatinine concentrations or renal pelvic dilation between the intravesical-MA and extravesical-SI techniques. Bladder mass height at the anastomosis site was significantly larger and persisted for longer with intravesical-MA technique. CONCLUSION: An extravesical-SI technique is seemingly the choice for ureteroneocystostomy in cats with undilated ureters. Renal pelvic dilation on ultrasound examination should be expected after ureteroneocystostomy in cats. CLINICAL RELEVANCE: An extravesical ureteroneocystostomy technique using a simple interrupted pattern for anastomosis should be considered in cats undergoing renal transplantation.     

抗生素/抗性细菌/抗性基因在土壤-植物系统中迁移转化及阻控消减的研究进展

土壤和植物是人类和动物生活中最重要的环境组成,但其正受到日益严重的人为资源污染,而进入环境的抗生素是主要的污染源之一。抗生素的过度使用和排放增加了土壤–植物生态系统和人类健康面临的抗生素污染及耐药性风险。阐明抗生素及其复合抗性污染物在土壤–植物系统中的环境行为及生态毒理学机理,对管控生态环境风险及保障食品安全具有重要的意义。本文综述了近年来国内外关于土壤–植物系统中抗生素的迁移转化规律、抗生素/抗性细菌/抗性基因的生态风险以及复合抗性污染物阻控消减技术的研究进展,以期为土壤–植物系统中抗生素抗性污染风险的管控和消减提供科学支撑。     

水利工程施工成本的控制

施工成本是工程成本控制的主要内容,水利水电工程施工成本有效控制是工程施工管理的核心,如何实现水利水电工程施工成本有效控制显的尤为重要。只要科学地组织和规划,采取适当的控制标准,严格的按规章实施,工程施工成本的控制就会取得良好的效果。     

低成本省力化栽桑技术的研究进展

我国已育成了若干适合条桑育,适于扦插的桑品种及适于种子直播的杂交桑,为建立高密度低成本桑园打下了基础。推广了直接用袋接体,硬枝扦插和杂交种直接建园和初试了中晚秋条桑收获技术。今后的研究方向是选育发根性强,高产优势,适合条桑收获的桑树品种及以条桑收获为主的提高劳动生产率的栽培技术。     

建立完善我国优质牛肉生产全程质量控制体系

本文介绍了我国牛肉生产和进出口现状及影响因素，并建议要制定推行我国牛肉生产全程质量控制体系以提高我国牛肉生产水平、牛肉质量及市场竞争力。     

侧柏毒蛾的发生情况及防治方法初探

侧柏毒蛾是危害侧柏、蜀桧的重要害虫,本文对该虫的发生情况和各虫期的危害特点进行了观察研究,根据其生物学特性提出了业、物理、生物、化学等方面的防治方法。     

保松灵防治松墨天牛的林间试验

松材线虫病 [Bursaphelenchus xylophilus (Steiner et Buhrer) Nickle]严重威胁我国的森林资源。浙江等地每年因该病造成大量松树枯死 ,防治松材线虫病刻不容缓。当前 ,我国防治该病主要措施是清理枯死松树 ,降低媒介昆虫松墨天牛 (Monochamus alternatus Hope)密度 ,减缓病害扩散蔓延。但在风景名胜区的松林和古树名木等 ,一旦发生该病 ,仅清理死树是不够的 ,必须采取保护性措施 ,防止因病害导致大量宝贵松树枯死。在日本 ,长期以来主要措施是在松树树冠进行空中或地面喷洒杀螟松、倍硫磷、西维因等农药 ,杀灭松墨天牛成虫 ,从而防治松…     

利用反向遗传技术拯救H9N2重组病毒

为研制高效特异的H9N2亚型AI疫苗,选取我国具有代表性的毒株A/chicken/Shanghai/10/01(H9N2)(简称SH10),以12质粒系统为基础,利用反向遗传操作技术构建了1株表面基因由SH10提供、内部基因由12质粒系统提供的重组病毒SH/PR8,为新型疫苗的研制提供了新的毒株。