微波处理松材线虫病疫木技术研究

该文对利用微波处理松材线虫病疫木技术进行了研究，结果表明：不论木材厚薄大小、含水量多少、在微波处理炉腔内怎样堆放，以及环境温度、微波功率等因素的如何变化，只要SWB-Ⅱ型隧道式微波除害处理设备显示的木材表面温度大于68℃，持续30min，就能有效地杀死松木中的松褐天牛和松材线虫。     

菠萝凋萎病毒研究进展

菠萝凋萎病毒的研究对于从根本上控制该病害具有重要意义。 文章介绍了菠萝凋萎病的症状和危害, 综述了菠萝凋萎病毒的分类、 检测、 基因组结构、 致病机制和防治策略等方面的研究现状和动向, 对存在问题进行了探讨。     

番茄斑萎病毒病抗性种质筛选及人工接种鉴定方法优化

对154份番茄材料(包括普通番茄76份,樱桃番茄78份)进行两年两次田间番茄斑萎病毒病的病情指数调查,筛选出14份对番茄斑萎病毒病具有稳定抗性的番茄材料,利用sw-5-2共显性SCAR标记对田间表现抗病的材料进行分子标记鉴定,发现3份抗病材料携带抗番茄斑萎病毒病的Sw-5基因。为缩短番茄斑萎病毒病人工接种鉴定周期,以含有sw-5的抗病材料H8和感病材料M82为研究对象,设置4、6、8、10片真叶4个接种时期,分别在接种后14、21、28 d进行病情指数调查和抗性分级,结果表明,6片真叶期接种,接种后28 d进行病情调查即可有效鉴别植株番茄斑萎病毒病抗性,与8、10片真叶期接种效果相同,人工接种抗病性鉴定效率显著提升。     

临安市竹笋加工业持续发展对策初探

分析了临安市发展竹笋加工业的优势及存在的问题，提出促进其持续发展的对策。     

混交林纯林中毛竹出笋退笋特性研究

应用出笋量的逻辑斯谛 ( L ogistic)模型研究了出笋量随时间增长的变化规律 ,结果表明 :出笋前期 (前 1 0 d)出笋数占总数的 1 5%左右 ,出笋中期 (第 1 0～ 2 0天 )占 75%,出笋末期 (第2 0天以后 )占 1 0 %;对退笋特征的观察及其规律的研究得出 :出笋前后期退笋数量少但退笋率高 ,而出笋中期退笋数量多但退笋率低 .小于 1 0 cm的浅鞭竹笋多属退化笋 .纯林中的出笋比混交林中的出笋持续天数短 ,退笋率高 ,为此混交林更有利于竹笋的生长 .出笋退笋规律可用来指导生产 ,为留养和挖取竹笋数量提供依据     

青蒿中番茄斑萎病毒和烟草花叶病毒的分子鉴定及相关序列分析

番茄斑萎病毒(tomato spotted wilt virus, TSWV)和烟草花叶病毒(tobacco mosaic virus, TMV)是2种重要的植物病原病毒, 对多种经济作物的产量和品质均造成严重影响。2021年-2022年, 在云南省丽江市烟草种植区不同烟区采集叶片黄化、皱缩以及无症状的青蒿Artemisia caruifolia样品共计14份, 利用免疫金标速测卡和RT-PCR对其病原病毒进行检测。利用免疫金标速测卡检测结果显示, 在所检样品中有9份样品检测出TSWV, 检出率为64.28%, 有3份样品检测出TMV, 检出率为21.43%, 2种病毒复合侵染的检出率同样为21.43%;利用RT-PCR对复合侵染的3份样品进行分子检测, 结果显示, 在3份复合侵染青蒿样品中获得3条TSWV N基因序列、3条TMV cp基因序列和2条TMV RdRp部分序列。TSWV青蒿分离物与分离自云南的TSWV-2分离物相似性最高, 为99.6%;TMV青蒿分离物与分离自辽宁的TMV-Shenyang分离物和分离自云南的TMV-Yongren-1相似性最高, 均大于99.4%。这是首次发现TSWV和TMV 2种不同属病毒复合侵染青蒿。     

玫瑰引种及嫩枝扦插试验初报

通过玫瑰引种栽培试验,观测引种玫瑰的形态特征、生态适应性和其主要物候期;嫩枝扦插试验结果为NAA500mg/L和IBA250mg/L溶液处理玫瑰嫩枝插穗,能促进插穗生根;草炭:素沙=1:1混合基质能促进玫瑰扦插苗生长;筛选出四季玫瑰、转基因中天粉红和转基因中天粉白等适宜嫩枝扦插繁殖的玫瑰品种。     

Biocontrol potential of Pseudomonas azotoformans,Serratia marcescens and Trichoderma virens against Fusarium wilt of Dalbergia sissoo

Wilt disease caused by Fusarium solani is a serious constraint to Dalbergia sissoo (shisham) plantations in northern India. In this study, the antagonistic potential of 40 bacterial isolates recovered from rhizophere soil of healthy shisham trees, and a well‐characterized Trichoderma species (Trichoderma virens) were tested for their possibility as biocontrol agents for F. solani. Two promising isolates (S1 and S15) were identified which inhibited pathogen growth, caused chitin degradation, produced siderophores and solubilized phosphate in vitro. Isolate S15 scored highest for hydrogen cyanide (HCN) production while isolate S1 was a non‐HCN producer. These two isolates were identified as Serratia marcescens (S1) and Pseudomonas azotoformans (S15) following sequence analysis of 16S rDNA. In dual culture assays, T. virens caused 80% inhibition of mycelial growth of the test fungus. The three selected antagonists when tested in planta in the glasshouse completely suppressed production of wilt symptoms on 12‐month‐old shisham plants. Further work is needed to ascertain the potential of these isolates to be used as biocontrol agents to manage shisham wilt under field conditions.     

Influencing in vitro clonal propagation of Chonemorpha fragrans(moon) Alston by culture media strength,plant growth regulators,carbon source and photo periodic incubation

Chonemorpha fragrans is an endangered medicinal woody climber,regarded among alternative plant sources of camptothecin.Camptothecin is a monoterpene indole anti-cancer alkaloid with annual trade value of over three billion U.S.dollars in the recent,and is used in the production of its analog drugs approved for the chemotherapy of cancer of varied types.Effects of plant growth regulators,culture media strength and photoperi-odic duration on the micropropagation ef ciency of C.fragrans from nodal segment explants were studied on Murashige and Skoog(MS)medium amended with Thidiazuron(TDZ),Benzylaminopurine(BAP)or Kinetin(Kin).Thidiazuron was more ef cient over BAP and Kin when half basal MS medium was used over full or quarter strength.Results of carbon source experiment showed sucrose as the most effective over glucose,fructose,and maltose in the clonal production.Studies on the photope-riodic incubation duration showed 12 h as the best light period and sub or supra-optimal resulted in the production of abnormal and albino micro shoots.Experimental results on the evaluation of physiological,biochemical parameters showed the role of pigment molecules and antioxidant systems in the production of albino micro shoots.