Expression of receptor tyrosine kinases VEGFR-1 (FLT-1), VEGFR-2 (KDR), EGFR-1, PDGFRa and c-Met in canine primary brain tumours

Inhibition of tumour growth and angiogenesis by targeting key growth factor receptors is a promising therapeutic strategy for central nervous system tumours. Characterization of these growth factor receptors in canine primary brain tumours has not been done. Using quantitative real‐time TaqMan polymerase chain reaction (PCR), we evaluated the expression of messenger RNA (mRNA) for five tyrosine kinase growth factor receptors (vascular endothelial growth factor receptor [VEGFR]‐1, VEGFR‐2, endothelial growth factor receptor [EGFR]‐1, platelet‐derived growth factor receptor a [PDGFRa], and c‐Met) relative to normal cerebral cortex in 66 spontaneous canine primary brain tumours. Increased expression of VEGFR‐1 and VEGFR‐2 mRNA was greatest in grade IV astrocytomas (glioblastoma multiforme) and grade III (anaplastic) oligodendrogliomas. EGFR‐1 mRNA expression was more consistently increased than the other receptors in all tumour types, while increased PDGFRa mRNA expression was mostly restricted to oligodendrogliomas. The similarities in increased expression of these tyrosine kinase growth factor receptors in these canine tumours, as compared to data from their human counterparts, suggest that common molecular mechanisms may be present.     

Primary lens luxation in the Chinese Shar Pei: clinical and hereditary characteristics

A pedigree analysis of a family of 15 related Chinese Shar Peis was conducted. This pedigree analysis, including affected and nonaffected dams, sires and offspring, was compiled to document and characterize the occurrence, common clinical signs, and age of onset of primary lens luxation while suggesting a possible mode of inheritance in this breed. Of the five offspring from the mating of an affected dam to two unrelated affected males, 100% of offspring were affected with bilateral primary lens luxations. Of the four viable offspring from the mating of the same affected dam to an unrelated, unaffected male, two dogs (50%) were affected. The average age of onset of affected animals (seven) in this first generation was 4.9 years (range 3–6 years). The six dogs in the second generation of the same pedigree line were 2-years-old at examination with none of these animals affected at the time of this study. The most common presenting complaints were a unilateral change in ocular appearance (5 of 7 dogs) and subjective vision impairment (4 of 7 dogs). The most common clinical sign upon ophthalmic examination was iridodonesis (unilateral 4 of 7 dogs; bilateral 3 of 7 dogs) and the presence of an aphakic crescent (3 of 7 dogs). Gonioscopy and tonometry of severely affected eyes revealed a narrow or closed iridocorneal angle and ocular hypertension. This study suggests that primary lens luxation does occur in the Chinese Shar Pei, resembling the clinical condition (age of onset, clinical signs) previously described in the terrier breeds, the Border Collie, and the Tibetan Terrier. Application of the phenotypic findings in this study to a Mendelian genetic model of inheritance suggests that primary lens luxation in the Chinese Shar Pei is inherited as a simple autosomal recessive trait.     

俄罗斯细毛羊毛肉兼用型品种的选育方法及生产性能

介绍了俄罗斯 7个细毛羊毛肉兼用型品种的选育方法及生产性能。     

浅谈市政道路石灰土基层施工

笔者从石灰土在道路施工中的重要性出发,分析市政道路石灰土基层施工的前期准备工作,并介绍了市政道路石灰土基层施工工艺流程,最后分析了特殊季节道路石灰土基层施工的注意事项。     

喀什地区棉田障碍因素调查及对策研究

经过 3a的调查研究 ,摸清了喀什地区棉田主要障碍因素 ,并针对性地进行了对策研究 ,使低产田改良成效显著 ,为棉花高产优质高效栽培技术提供了理论依据。     

母牛子宫内膜炎的防治

本文分析了张掖市百万头肉牛基地母牛子宫内膜炎的发生原因，并根据临床症状讲述了诊断的依据，总结了有效的治疗方法及作者的一些临床经验和治疗体会，提出了预防措施，在母牛保健方面可供参考。     

对乙酰氨基酚对大鼠原代肝细胞的损伤机制

胶原酶两步灌流法获取SD大鼠的原代肝细胞,并用不同剂量的对乙酰氨基酚处理.MTT法测定肝细胞的存活率,分光光度法测定培养上清液中乳酸脱氢酶(LDH)的含量.Western blotting法测定大鼠肝细胞中细胞色素P450酶2E1(CYP2E1)的表达水平.结果表明,对乙酰氨基酚可导致肝细胞存活率降低,LDH含量显著升高,CYP2E1表达增加.说明达到一定的剂量时.对乙酰氨基酚可引起肝细胞的损伤.     

"中华红又亮"对断奶仔猪的饲喂效果试验

试验选用体重约8kg的三元杂交猪(杜×长×大)60头,随机分成3组,每组2个重复,分别饲喂含不同剂量"中华红又亮"的饲粮,研究"中华红又亮"对仔猪生产性能、肤色的影响.结果表明,以"中华红又亮"取代饲粮中部分硫酸亚铁,可显著提高仔猪平均日增重(P＜0.05),显著降低料重比(P＜0.05),明显改善仔猪肤色.根据试验效果,仔猪饲粮中"中华红又亮"的添加浓度宜为3.0x10-.4 6.0x10-4.     

柔嫩艾美球虫（Eimeria tenella）体外细胞培养

试验比较了玻璃珠层析法，ＤＥＡＥ纤维素层析法，Ｇ３漏斗过滤法３种提纯柔嫩艾美球虫（Ｅｉｍｅｒｉａｔｅｎｅｌｌａ）子孢子的方法，柱高８ｃｍ，直径２００μｍ的玻璃株柱用ｐＨ８．０的Ｒｉｎｇｅｒ’ｓ缓冲液作洗脱提纯子孢子，回收率为６４．１％，子孢子洗脱高峰期集中，且易于无菌操作，柱高５ｃｍ的ＤＥＡＥ纤维素柱用ｐＨ８．０的甘氨酸缓冲液作洗脱液，提纯子孢子回收率为６３．２％，但严格无菌操作困难，Ｇ３漏斗法易