罗汉果生产技术研究的思路与发展对策

探讨了罗汉果生产技术研究思路，提出选育、引进优良品种，培育茎尖脱毒壮苗，建立种薯基地，采用容器无土栽培、平地栽培、设施栽培，施用天敌真菌、接种VA菌根真菌防治根结线虫等技术建议。     

模糊隶属法对玉米苗期耐旱性的拟合分析

在略高于玉米萎蔫系数的干旱条件下,以出苗率和生物学产量耐旱系数之乘积为指标,测定了17个玉米自交系和10个杂交种的苗期抗旱性。并用模糊隶属法,以干旱胁迫下的胚芽鞘长度,出苗率,根重,生物学产量,脯氨酸含量,电导率和离体叶片保水力等指标对各品种耐旱性进行了拟合分析。结果表明,在略高于萎蔫系数的干旱条件下,以出苗率和生物学产量的耐旱系数之乘积为指标,可以准确鉴定玉米苗期的耐旱性,与耐旱性的综合评价拟合良好(相关系数r=0.914)。     

辽西人工林资源状况分析

采用资料收集和实地调查相结合的方法,系统分析了辽西地区人工林资源状况,明确了这一地区人工林资源的分布和结构特征以及存在的主要问题,为今后辽西林业的可持续发展提供依据。     

Suppression of wheat-seedling diseases caused by Fusarium culmorum and Microdochium nivale using bacterial seed treatment

Snow mould, caused by Microdochium nivale , and seedling blight caused by members of the Fusarium complex, are cereal diseases of great economic importance in many temperate zones. In a glasshouse bioassay designed to enhance disease, about 600 plant-associated bacterial isolates obtained by different methods were screened for suppressive effects in wheat against infection caused by Fusarium culmorum . Although most of the isolates tested had a neutral effect on test plants and disease development, a few were synergistic to the pathogen and about one-fifth showed > 80% disease suppression. During five consecutive growing seasons, 164 bacterial isolates were tested in field experiments against both F. culmorum and M. nivale as causal agents of seedling blight. Tests for effects on yield in experiments with spring and winter wheat, performed in different climatic regions of Sweden, showed that disease-suppressive effects were repeatable. The most efficient isolates, three fluorescent pseudomonads and a species of Pantoea , suppressed disease equal to that of the fungicide guazatine, both with respect to crop stand and yield. Seed treatment with Pantoea sp. (isolate MF 626) increased yield by an average of more than 500 kg ha⁻¹ in six field experiments.     

低温胁迫下弓葵幼苗膜脂过氧化及保护酶活性的变化

 低温胁迫下弓葵( Butia capitata Becc) 幼苗叶片的MDA 含量逐渐增加, 膜脂过氧化作用增强。- 8 ℃条件下的膜脂过氧化作用明显强于2 ℃。细胞膜透性在2 ℃条件下变化不大, - 8 ℃时则随低温胁迫时间延长而急剧上升, 细胞膜受到伤害。- 8 ℃胁迫下细胞保护酶SOD、POD 和CAT 活性短期(6 h) 内升高,然后下降, 24 h 以后3 种保护酶受到低温胁迫的严重抑制。在2 ℃胁迫下, SOD 活性在6 h 内变化不大, 随后下降; CAT活性变化趋势与- 8 ℃时相似, 但变化幅度较小; POD 虽也呈现先升后降的趋势, 但降幅明显小于升幅, 至48 h 时POD 活性仍维持较高水平。2 ℃低温胁迫不是抑制而是促进POD 活性的提高。     

烯效唑浸种对黄瓜的壮苗效应

研究不同浓度烯效唑浸种对黄瓜幼苗生长发育的影响。结果表明,烯效唑可抑制黄瓜幼苗徒长,使株高降低,比叶重增加,根冠比和壮苗指数增大,叶绿素含量增加,根系活性增强,在形态上和生理上表现出显著的壮苗效应;烯效唑对黄瓜有极显著的增产作用,以20 mg·kg-1处理最佳,促进增产的主要原因是提高了单株结瓜数。     

Aerial seed germination and morphological characteristics of juvenile seedlings in Commelina benghalensis L.

Seed germination, seedling emergence, and the morphological characteristics of juvenile seedlings of Commelina benghalensis L. were observed. For aerial seeds collected in September and October, seedling emergence peaked in April and June for large seeds and from June to August for small seeds, whereas seedling emergence for large seeds collected in November showed peaks in March and April under natural rainfall conditions, and in April and June under irrigation conditions. Seedlings from small seeds emerged intermittently over a longer period from April to August under both conditions. Aerial seeds of C. benghalensis germinated on wet filter paper on the second day after seeding (DAS) for large seeds and the fourth DAS for small seeds. The germination percentage for large seeds was higher than that for small seeds by the 14th DAS. The germination percentage for large aerial seeds showed no significant difference between light and dark conditions. However, the percentage for small aerial seeds was higher under light than under dark conditions. Seedlings from large aerial seeds emerged on the third DAS at 0–50 mm soil depths. The percentage of emergence at 0 and 1 mm soil depths increased until the 30th DAS, whereas those at soil depths of 5–50 mm showed no change after the 9th DAS. There was no emergence at a soil depth of 100 mm. Seedlings from small aerial seeds emerged on the 6th DAS at 0–1 mm soil depths, with the percentage increasing until the 30th DAS. Although seedlings at 5 and 10 mm soil depths also emerged on the 6th DAS, there was no change in the percentage after the 12th DAS. There was no emergence at soil depths of 20–100 mm. The hypocotyl and taenia (part of the cotyledon connected to the seed) in juvenile seedlings that emerged from soil depths of 50 mm were longer than those in seedlings emerging from a soil depth of 1 mm.     

猪繁殖与呼吸综合征病毒缺失变异株的基因组特征

对猪繁殖与呼吸综合征病毒(PRRSV)分离株HB2(sh)／2002的全基因组序列进行了测定与分析。该毒株基因组全长为15373nt(不包括PolyA尾)，与国内外美洲型PRRSV分离株全序列相似性介于88．7％～95．1％之间。序列分析表明，该毒株是1个天然存在缺失的变异毒株，其ORFla的Nsp2存在编码12个氨基酸的连续36个核苷酸的缺失，ORF、3存在编码1个氨基酸的3个核苷酸的缺失。这是国内外首次发现PRRSV存在缺失变异现象，研究结果补充和丰富了PRRSV毒株的基因组信息数据，为深入研究该毒株的遗传与变异及其与生物学特性的关系奠定了基础。     

UHPLC-Q/TOF MS结合HPLC-DAD定性和定量分析硫酸黏菌素可溶性粉中的未知添加物

定性和定量分析一批兽药硫酸黏菌素可溶性粉中的未知添加物。照《中国兽药典》2010年版一部对该批检品用微生物检定法进行含量测定时,发现该样品的抑菌圈为虚圈,用薄层色谱鉴别该样品,未显示与标准品溶液一致的主斑点,怀疑该样品中有处方外非法添加物。采用超高效液相色谱-四级杆-飞行时间质谱(UHPLC-Q/TOF MS)对该样品进行筛查,发现疑似添加物,并使用液相色谱-二极管阵列检测(HPLC-DAD)法进行了双重确证和含量测定。该样品中非法添加物确证为磺胺氯达嗪和甲氧苄啶,添加量分别为58.5 mg/g和13.4 mg/g。本研究通过建立筛查方法为监管部门提供技术支撑,通过分析非法添加物的可能原因为打击兽药处方外非法添加提供了思路。     

川西北高寒沙地不同年限高山柳林下优势植物碳、氮、磷生态化学计量特征

通过采集川西北高寒沙地不同年限(6、18、24年)高山柳林下3种优势植被藏沙蒿、裂叶独活和镰荚棘豆,分别测定分析3种植被叶片、根部C、N、P化学计量特征变化特征。结果表明:不同年限高山柳林下植被C、N、P含量及其比值间存在显著差异且呈现出不同变化趋势。林下植被C含量整体下降;叶N含量呈上升趋势,根N含量随年限增长而下降;除藏沙蒿外,林下植被P含量变化不显著;C∶N变化范围为1.92~12.86;C∶P为29.18~196.88;不同年限高山柳林下植被N∶P间虽存在差异,但均表现出主要受到P限制,表明该区域植被生长主要受P限制,应注意P养分的适当补充。